Method for producing L-lactic acid through biomass fermentation

A biomass fermentation and biomass technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem of high production cost of lactic acid, and achieve the effect of reducing alkali consumption and wastewater discharge

Active Publication Date: 2011-09-07
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the amount of alkaline neutralizer used in lactic acid fermentation production is an important factor leading to higher production costs of lactic acid

Method used

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  • Method for producing L-lactic acid through biomass fermentation
  • Method for producing L-lactic acid through biomass fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Weigh 1 kg of corncobs, crush them into particles of about 1 to 2 cm, add 0.5 kg of sodium hydroxide and 7.5 kg of water, cook at about 100° C. for 60 minutes, press filter to separate the cooking liquid and residue, and obtain alkali treatment waste liquid I and Alkaline Treatment of Corncob Residue II.

[0029] Prepare 2L simultaneous saccharification and fermentation medium in a 3L saccharification and fermentation tank: absolute dry alkali treatment corncob residue II 150g / L, yeast extract 5g / L, ammonium chloride 1g / L, MgSO 4 0.5g / L, pH natural. Add 20 mL of cellulose degrading enzyme with an enzyme activity of 150 FPIU / mL (cellulolytic enzyme Celluclast is Novozymes commercial enzyme, produced by Trichoderma reesei ATCC 26921), heat up to 50°C, stir at 200 rpm, and perform hydrolysis for 6 hours.

[0030] Pre-cultivation of Bacillus coagulans: Add Bacillus coagulans (DSM 23183) into 200 mL of seed medium, and cultivate for 18 hours at 180 rpm and 50° C. without ve...

Embodiment 2

[0035] Weigh 3kg of corn stalks, cut them into pieces of about 1-2cm, add NaOH solution with a concentration of 5% (w / w) according to the solid-liquid weight ratio of 1:10, cook at about 90°C for 120min, centrifuge at 3500rpm for 15min to solidify The liquid is separated to obtain the alkali-treated waste liquid I and the alkali-treated corn stalk residue II.

[0036] Prepare 3L simultaneous saccharification and fermentation medium in a 5L saccharification and fermentation tank: Alkali-treated corn stalk residue II 200g / L, yeast extract 7g / L, ammonium chloride 1.2g / L, MgSO 4 0.5g / L, pH natural. Add 20 mL of cellulose-degrading enzyme with an enzyme activity of 150 FPIU / mL (derived from Trichoderma koningeni), heat up to 50° C., stir at 200 rpm, and perform hydrolysis for 6 hours.

[0037] Pre-cultivation of Bacillus coagulans: Inoculate Bacillus coagulans (DSM 23183) into 300mL of fresh seed medium, and cultivate for 18h at 180rpm, 50°C without ventilation. Seed medium form...

Embodiment 3

[0042] Weigh 1kg of bagasse after sugar industry pressing, add NaOH with a concentration of 3% (w / w) according to the solid-liquid weight ratio of 1:10, cook at about 100°C for 60min, centrifuge at 3500rpm for 15min for solid-liquid separation, and obtain alkali treatment Waste liquid I and alkali-treated sugarcane residue II.

[0043] Prepare 2L simultaneous saccharification and fermentation medium in a 3L saccharification and fermentation tank: Alkali-treated bagasse 100g / L, corn steep liquor powder 5g / L, ammonium chloride 1g / L, MgSO 4 0.5g / L, pH natural. Add 13 mL of cellulose degrading enzyme with an enzyme activity of 150 FPIU / mL (the cellulose degrading enzyme Celluclast is a Novozymes commercial enzyme, produced by Trichoderma reesei ATCC 26921); the temperature is raised to 50° C., the stirring speed is 200 rpm, and the hydrolysis reaction is carried out for 3 hours.

[0044] Pre-cultivation of Bacillus coagulans: Inoculate Bacillus coagulans (DSM 23183) into 200 mL ...

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Abstract

The invention discloses a method for producing L-lactic acid through biomass fermentation. The method comprises the following steps: after the biomass is subjected to alkaline liquid heat treatment, performing solid-liquid separation to obtain a liquid I and residues II; adding a culture medium containing the residues II into a saccharification fermentation tank, adding a cellulose degradation enzyme and performing a hydrolysis reaction for 0.5-6 hours at 50-55 DEG C; keeping the temperature of the saccharification fermentation tank at 50-55 DEG C; adjusting the pH value of the culture medium to 6.0-6.5 by using the liquid I; inoculating bacillus coagulans into the saccharification fermentation tank; and performing synchronous saccharification fermentation on the residues II for 48-96 hours in an anaerobic condition to produce L-lactic acid, and adding the liquid I in flow to keep the pH value of the fermentation liquid at 5.2-5.8. Compared with the prior art, the method disclosed by the invention not only can improve the comprehensive efficiency of converting cellulose and hemicellulose into lactic acid, and adopts an alkaline waste liquid to neutralize lactic acid in the fermentation process so as to reduce alkali consumption and waste water discharge in the lactic acid production industry.

Description

technical field [0001] The invention belongs to the field of biomass utilization and development, and in particular relates to a method for producing cellulose L-lactic acid directly by one-pot fermentation of alkaline biomass residues without additional addition of acid and alkali to regulate pH. Background technique [0002] Lactic acid, also known as 2-hydroxypropionic acid, is an important multipurpose organic acid that can be widely used in food, medicine, agriculture and chemical industries. Since lactic acid contains an asymmetric carbon atom, it can be divided into L-type and D-type. Among them, L-lactic acid has a wide range of applications, and its production research has received widespread attention. [0003] Industrial production of lactic acid includes fermentation and chemical methods. The chemical method uses acetaldehyde and hydrocyanic acid as raw materials to synthesize it. The production cost is high, the environmental pollution is serious, and it is di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56C12P19/14C12R1/07
Inventor 欧阳嘉蔡聪李鑫朱均均勇强
Owner NANJING FORESTRY UNIV
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