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Microbial degrading agent for decomposing cassava stalks and preparation method

A technology for microbial degradation and cassava stalks, which is applied in the direction of biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of degrading cassava stalks, achieve rapid degradation, low cost of use, and improve the effect of soil structure

Inactive Publication Date: 2011-09-07
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reported straw decomposing agents are all microbial preparations aimed at degrading corn, rice and other straws. So far, there is no microbial degrading agent aimed at degrading cassava stalks. How to develop microbial preparations suitable for the degradation of cassava stalks? Degrading bacteria with a strong decomposing effect, and organically cooperating with them to produce efficient and stable cassava stalk degradation agents are urgent tasks

Method used

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  • Microbial degrading agent for decomposing cassava stalks and preparation method
  • Microbial degrading agent for decomposing cassava stalks and preparation method

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Experimental program
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Effect test

Embodiment 1

[0015] Purchased from the China Industrial Microbiology Culture Collection Center (CICC): Phanerochaete chrysosporium (No. 40719), white rot fungus (No. 40299), Puccinia tufts (No. 40259), Aspergillus fumigatus (No. 2434) ), Bacillus liquefaction (No. 20651), Trichoderma reesei (No. 41492), Aspergillus fig (No. 2420).

[0016] After the above strains were expanded and cultivated respectively, the fungi (filaments) were collected, and combined according to the following proportions based on the biomass of the bacteria: 10% of Phanerochaete chrysosporium, 20% of white rot fungus, and Mold 10%, Aspergillus fumigatus 15%, Bacillus thermophiles liquefaction 20%, Trichoderma reesei 15%, Aspergillus fig 10%. Using wheat bran as the bacterial adsorbent, mix evenly according to the ratio (weight of bacteria: weight of wheat bran=1:10) to prepare microbial degradation bacterial agent.

[0017] The cassava stalks were pulverized, and two groups were set up to carry out the experiment: t...

Embodiment 2

[0021] The cassava rod-degrading bacteria were isolated from the cassava planting soil and cassava rod rotting piles. After separation, purification and identification, the lignin-degrading bacteria were: Aspergillus flavus ( Aspergillus flavus ) and Bacillus licheniformis ( Bacillus licheniformis ); the hemicellulose-degrading bacteria are: Bacillus subtilis ( Bacillus subtilis ) and Aspergillus niger ( Aspergillus niger ); Cellulose-degrading bacteria: Penicillium decumbens ( Penicillium decumbens ), Trichoderma viride ( Trichoderma viride ), Trichoderma korningii ( Trichoderma koningii ).

[0022] After the above strains were expanded and cultivated respectively, the fungi (filaments) were collected and combined in the following proportions based on the biomass of the bacteria: Aspergillus flavus 15%, Bacillus licheniformis 20%, Bacillus subtilis 10%, Aspergillus niger 15%, Penicillium recumbens 10%, Trichoderma viride 20%, Trichoderma korningen 10%. Wheat bran...

Embodiment 3

[0028] The lignin-degrading bacterium White-rot fungus; the hemicellulose-degrading bacterium Bacillus liquefier; and the cellulose-degrading bacterium Trichoderma reesei were purchased from China Agricultural Microorganism Culture Collection Center (ACCC). The lignin-degrading bacteria Aspergillus flavus, the hemicellulose-degrading bacteria Bacillus subtilis and the cellulose-degrading bacteria Trichoderma viride were isolated and screened from the rotten pile of cassava stalks.

[0029] After the above-mentioned strains were expanded and cultivated respectively, the fungi (filaments) were collected, and combined in the following proportions based on the biomass of the bacteria: 10% of white rot fungus, 20% of Aspergillus flavus, 20% of thermophilic bacillus liquefaction, Bacillus subtilis 10%, Trichoderma reesei 15%, Trichoderma viride 25%. Wheat bran was used as the bacterial adsorbent and mixed evenly according to the ratio (cell weight: wheat bran weight = 1:10) to prepa...

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Abstract

The invention discloses a microbial degrading agent for decomposing cassava stalks and a preparation method. The microbial degrading agent for decomposing the cassava stalks is characterized in that: microbial strains of the microbial degrading agent consist of the following components in percentage by taking bacterial biomass as a counting unit: 10 to 50 percent of lignin degradation flora, 10 to 50 percent of hemicellulose degradation flora and 10 to 50 percent of cellulose degradation flora, wherein each strain is purified, rejuvenated, propagated and cultivated; each cultivated strain is compounded according to the proportion; a microbial adsorbent is added; and the components are uniformly mixed to prepare a solid microbial degrading agent. The microbial degrading agent has high efficiency and pertinence; the cassava stalks can be quickly degraded; environmental pollution caused by piling, incineration and the like is avoided; the cassava stalks which are decomposed by the microbial degradation bacteria are changed into things of value to prepare a fertilizer so as to increase both production and income; the microbial degrading agent is easy to operate and is low in using cost; and positive effects of improving soil structure, reducing environmental pollution and the like are achieved when the degrading agent is popularized and applied at cassava planting areas.

Description

technical field [0001] The invention relates to a microbial treatment technology and a preparation method for degrading crop straws. Background technique [0002] Now the international community is advocating the production of fuel ethanol from non-grain and cellulose straws, and the production of fuel ethanol from cassava has great advantages in terms of cost and efficiency, and has great development potential. The world's cassava planting area is about 15 million hectares, with a total output of 170 million tons. my country's cassava output was about 3 million tons in 2006, and it has reached more than 25 million tons in 2009. At present, my country's cassava is in short supply and has become the world's largest. A large cassava importing country will continue to expand the cassava planting area to meet the domestic market. However, a large number of cassava stalks are left in the harvested cassava fields. Because the cassava stalks contain a lot of lignocellulose and are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12P7/10
CPCY02E50/16Y02E50/10
Inventor 余勃陆豫马春燕唐楚颖
Owner NANCHANG UNIV
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