Raw trichoderma asperellum strain, bactericide and application of raw trichoderma asperellum strain and bactericide in treatment of organic waste

A technology of Trichoderma aculeatus and Trichoderma, applied in the biological field, can solve problems such as inability to achieve decomposition effects and complex material components, and achieve the effects of reducing soil-borne diseases, low preparation costs, and reducing cellulose content

Active Publication Date: 2015-03-25
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, due to the complex material composition in the garbage, including nutrients that are easily used by microorga

Method used

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  • Raw trichoderma asperellum strain, bactericide and application of raw trichoderma asperellum strain and bactericide in treatment of organic waste

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: the acquisition of Trichoderma aculeatus T-1

[0038] The Trichoderma aculeatus strain involved in the present invention is isolated from soil by carboxymethyl cellulose plate and dilution coating method, and is determined to be an efficient cellulose degrading bacteria by Congo red staining and filter paper strip decomposition test, and then undergoes physiological Characteristic analysis and ITS rDNA identification confirmed that it was Trichoderma aculeatus, and the specific steps were as follows:

[0039]1. Separation: Weigh 10g of the soil sample, put it into a triangular flask filled with 90ml of sterile water, shake it for about 20 minutes, that is, the soil solution with a dilution of 10-1, use a pipette to draw 1ml of the soil suspension into it In a test tube containing 9ml of sterile water, blow and suck three times, try to mix thoroughly, that is, a soil solution with a dilution of 10-2, then draw 1ml of the solution from the test tube and inje...

Embodiment 2

[0044] Embodiment 2: the plate antagonism experiment of Trichoderma aculeatus T-1

[0045] Use the confrontation culture method to prepare a PDA plate, use a puncher to punch a small piece of bacterial cake at the edge of Trichoderma aculeatus and the bacteria to be antagonistic, inoculate it into the center of the opposite sides of the new PDA shield, culture at 28°C, and observe the Aechinospora Action of Trichoderma against antagonistic bacteria. The results showed that Trichoderma aculeatus T-1 could inhibit the growth of plant pathogens Fusarium oxysporum and Fusarium solani.

Embodiment 3

[0046] Embodiment 3: the preparation of Trichoderma aculeatus T-1 microbial bacterial agent

[0047] Using crop straw as the main substrate, Trichoderma aculeatus T-1 is cultivated by solid-state fermentation to prepare microbial inoculum. The specific preparation steps are as follows:

[0048] Strain activation culture, inoculate the slant preservation of Trichoderma aculeatus T-1 strain on a PDA plate, and culture it at 25°C-30°C for 3-5 days until a large number of spores are formed;

[0049] To prepare a solid medium, mix the straw with a crushed particle size of 20-40 mesh and bran according to the ratio of solid amount of 3:1, and then add Mandel culture solution, autoclaved at 121°C for 20 minutes, cooled as a solid medium for Trichoderma aculeatus T-1, in which Mandel culture solution contains NaNO 3 2g, KH 2 PO 4 1.5g, CaCl 2 0.3g, MgSO 4 ·7H 2 O 0.3g, FeSO 4 ·7H 2 O 0.005g, MnSO 4 ·H 2 O 0.0016g, ZnSO 4 ·H 2 O0.0014g, CoCl 2 0.0005g, water 1000ml, pH 6; ...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a biocontrol strain, namely trichoderma asperellum T-1, for highly producing composite cellulose degrading enzyme. The trichoderma asperellum T-1 is preserved at the China General Microbiological Culture Collection Center on September 24, 2014; and the preservation number is CGMCC 9722. The invention also provides a waste composting bactericide prepared from the trichoderma asperellum T-1 and an application of the waste composting bactericide in treatment of the organic waste. The trichoderma asperellum T-1 provided by the invention is rapid in growth, high in spore-producing ability, and high pH tolerance; a plurality of cellulose materials can be taken as substrates; and the trichoderma asperellum T-1 is a member of biocontrol bacteria, and can be applied to planting of crops to prevent diseases. By adding the trichoderma asperellum T-1 provided by the invention to the organic waste, the penetrating fluid generated by waste composting can be reduced; the degradation efficiency of the cellulose materials in the waste can be improved; and the quality of the waste composting product is improved.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a high-yield compound cellulase biocontrol strain Trichoderma aculeatus T-1 and a microbial agent containing the Trichoderma aculeatus, and its use in organic waste treatment and recycling Applications. Background technique [0002] With the acceleration of urbanization and the improvement of people's living standards, the amount of garbage generated in our country is constantly increasing. However, corresponding to this, the level of domestic waste treatment and pollution prevention and control in my country is still relatively lagging behind. How to realize waste recycling and reduce the amount of waste disposal has become an urgent problem to be solved. [0003] According to statistics, the organic waste produced in my country every year can reach 41.3-43.3×10 8 tons, including kitchen waste, livestock and poultry manure, garden clipping waste, straw crop st...

Claims

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Application Information

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IPC IPC(8): C12N1/14C05F17/00A01N63/04A01P3/00C12R1/885
CPCA01N63/30C05F9/04C05F11/08C05F17/00C12N1/14C12N1/145C12R2001/885Y02W30/40
Inventor 林辉符建荣马军伟夏芸赵宇华姜丽娜叶静王强俞巧钢孙万春邹平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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