Screening and domesticating method of composite microbial system for low-temperature degradation of straws

A technology of composite bacteria and straw, applied in the agricultural field, can solve the problems of limited promotion, poor decomposition effect, slow decomposition, etc.

A technology of composite bacteria and straw, applied in the agricultural field, can solve the problems of limited promotion, poor decomposition effect, slow decomposition, etc.

CN103087973AInactive Publication Date: 2013-05-08INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Screening and domesticating method of composite microbial system for low-temperature degradation of straws
  • Screening and domesticating method of composite microbial system for low-temperature degradation of straws
  • Screening and domesticating method of composite microbial system for low-temperature degradation of straws

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Screening of Composite Bacteria for Low Temperature Degradation of Corn Stalks

[0022] The experiment was carried out in the microbiology laboratory of Sarazi Experimental Base of Inner Mongolia Agricultural University from 2011 to 2012.

[0023] 1. Sources of materials and strains

[0024] Collect cellulose-rich sawdust, rotten grass, rotten straw, cattle, sheep, chickens, ducks, pigeons and rabbits, meadow soil, forest soil, wood mushroom, and soil that has been returned to the field for many years in the alpine area with an average annual temperature of -0.6-8°C 100 materials.

[0025] 2. Enrichment culture

[0026] In this experiment, filter paper laying method, filter paper disintegration method, and natural corn stalk powder shaking table enrichment culture were adopted.

[0027] Filter paper tiling method: Mix and enrich the collected materials for static culture, rotten corn stalks, rotten wheat stalks, rotten rice stalks, rotten fallen leaves, rot...

Embodiment 2

[0051] Example 2 Degradation Effect Determination of Domesticated Composite Bacteria Lines Corn Stalks

[0052] 1. Growth status of domesticated composite bacteria on corn stalk solid medium and its effect on corn stalk degradation

[0053] According to the inoculum amount of 5% (volume ratio), inoculate the liquid culture solution of the compound bacterial strain on the corn stalk, and cultivate the acclimatization line under the condition of 20 ℃, and observe that 1-2mm mycelium grows on the corn stalk on the 2nd day; On the 3rd day, the mycelia basically covered the upper part; on the 5th day, the mycelia still grew vigorously; on the 7th day, the mycelium grew slowly; on the 10th day, the mycelia showed autolysis, and most of them stopped growing. Cultivated at 15°C, 1, 8, 11, and 16 strains were observed to grow 1-2mm mycelia on the corn stalks on the second day; on the fourth day, the mycelia had basically covered the upper part; on the eighth day, the mycelium was still...

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Abstract

The invention discloses a screening and domesticating method of a composite microbial system for low-temperature degradation of straws. The screening and domesticating method comprises the steps of acquiring a natural material sample rich in cellulose in an alpine area in which the annual average temperature is -0.6-8 DEG C, carrying out enrichment culture on a cellulose degradation microbial system, then carrying out restrictive subculture preliminary screening, low-temperature gradient induced domestication and cellulase enzyme activity re-screening, and then carrying out low-temperature multi-generation restrictive carbon source subculture domestication to obtain the composite microbial system for low-temperature degradation of straws. The screening and domesticating method can be used for screening and domesticating the composite microbial system for high-efficiency degradation of straws at low temperature and has an important meaning in field returning and utilization of straw resources in the northern area.

Description

technical field [0001] The invention belongs to the technical field of agriculture (microorganisms), and in particular relates to a screening and domestication technology of crop straw low-temperature degradation compound bacterial strains. Background technique [0002] Crop straw is a rich natural resource. my country is a large agricultural country, with an annual output of more than 600 million tons of straw, of which only a small part is utilized. Straw returning to the field in situ is an effective means to improve soil organic matter, rationally utilize resources, and solve environmental pollution. However, after straw enters the soil, it decomposes slowly, which has many adverse effects on farming and agronomic operations, which limits its promotion. Especially in northern my country, due to the long winter time and low temperature, the slow degradation and poor decomposition effect of crop straw have become the bottleneck restricting the utilization of straw returnin...

Claims

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Application Information

Patent Timeline
08 May 2013
Publication
CN103087973A
IPC
C12N1/22; C12N1/02; C12N1/36
Inventors
高聚林; 萨如拉