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Bacillus amyloliquefacien and preparation method and application thereof

A technology for dissolving amyloid spores and bacilli, applied in biochemical equipment and methods, applications, microorganism-based methods, etc., to achieve the effects of reducing pollution, good antibacterial effect, and strong environmental tolerance

Active Publication Date: 2013-09-04
WUHAN KEQIAN BIOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on its application in China is limited to replacing organic pesticides, inhibiting plant pathogenic fungi and viruses and other biological control; and degrading agricultural waste, inhibiting the growth of algae in water and other fields of environmental governance.

Method used

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  • Bacillus amyloliquefacien and preparation method and application thereof
  • Bacillus amyloliquefacien and preparation method and application thereof
  • Bacillus amyloliquefacien and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Strain Isolation

[0026]Fresh feces from healthy pigs were collected from a natural pig farm in Huangpi, Hubei. The feces samples were enriched in nutrient broth (NB) medium for aerobic enrichment and turbidity. They were treated in a water bath at 80°C for 15 minutes, and then enriched and heated. Screen once, spread on plate after appropriate dilution, pick typical single colonies according to the colony shape (colonies are nearly round, 3-4.5mm, dull, central bulge, edge cog-toothed, off-white), and spores are screened out by microscopic examination bacilli.

[0027] The formula of NB medium (1L): beef extract 5g, peptone 10g, NaCl 5g, agar 15g.

[0028] Re-screening and identification

[0029] Screened against Staphylococcus aureus (G + ), Escherichia coli (G - ) and other pathogenic bacteria with good growth inhibition performance, through physiological and biochemical and 16s identification, a strain of Bacillus amyloliquefaciens was finally screened and name...

Embodiment 2

[0031] Example 2: Scale-up cultivation of Bacillus amyloliquefaciens H6

[0032] According to corn flour 15g / L, soybean meal 20g / L, yeast extract 10g / L, peptone 10g / L, CaCO 3 3g / L, NaCl5g / L, MgSO 4 2g / L, KH 2 PO 4 3g / L, polyether 2ml / L, and 65% (V / V) liquid content to prepare fermentation broth, sterilized by high-temperature steam at 121°C for 20min, then cooled to 40°C, inoculated with 1% seed liquid of 10h age, Cultivate at 32°C, control the stirring speed to maintain dissolved oxygen (DO value above 30%), and automatically add defoamer (silicone oil) online. Sampling every 2 hours to measure the OD value, fermented for 24 hours and placed in the tank, the plate count of Bacillus amyloliquefaciens H6 was 1.78×10 10 cfu / ml ( figure 2 ).

Embodiment 3

[0033] Embodiment 3: preparation of bacillus amyloliquefaciens bacteria powder

[0034] The bacteria liquid fermented in Example 2 is coarsely filtered through a vibrating sieve to remove large solids, and then concentrated to 1 / 6 of the original volume by microfiltration through a 0.2 μm ceramic membrane. The concentrated solution is directly pumped into the spray drying tower by a peristaltic pump. 180±3°C, outlet temperature 67-70°C, control the feed flow rate and heating switch, maintain temperature balance, calculate the bacterial powder output after receiving the material to be 40kg / m 3 Fermentation liquid, plate count bacterial powder bacterial count is 4.7×10 11 cfu / g.

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Abstract

The invention discloses a bacillus amyloliquefacien and a preparation method and application thereof. According to the bacillus amyloliquefacien and the preparation method and the application thereof, the culture collection number of the bacillus amyloliquefaciens H6 is CCTCC No: M2013249; the bacillus amyloliquefaciens has obvious growth inhibition effect for gram-positive bacteria and gram-negative bacteria, strong degradation ability for starch, albumen, and cellulose, strong environment tolerance, and excellent effect for preventing diarrhea and promoting growing, and can not produce adverse effect with large doses of feeding; and the rear condition is simple, the cost is low, and the development and application prospect is excellent.

Description

technical field [0001] The invention relates to the technical field of animal feed additives, in particular to a Bacillus amyloliquefaciens H6, a preparation method of the Bacillus amyloliquefaciens H6, and a Bacillus amyloliquefaciens H6 Application in the preparation of animal feed additives. Background technique [0002] It is the development trend of the world to completely ban the addition of antibiotics in feed: the European Union officially banned the addition of any antibiotics in feed in 2006; South Korea began to ban the use of feed antibiotics in 2010; Antibiotics are strictly restricted; to stop using antibiotic feed additives, alternatives that can replace antibiotic feed additives must be selected to ensure that the efficiency and benefits of animal husbandry production will not be affected. In this case, the development of microbial agents, the most promising feed additive as an antibiotic substitute, has attracted the attention of various countries. The ear...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16C12R1/07
Inventor 徐高原王杨波周明光金建云于福祥李芳蔡浩金梅林陈焕春
Owner WUHAN KEQIAN BIOLOGY CO LTD
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