Microbial bacterium agent for degrading Chinese kitchen refuses and preparing method thereof
A technology for microbial inoculants and kitchen waste, applied in the field of microorganisms, can solve the problems of less application, retention, and impact on compost quality, and achieve the effects of avoiding incompleteness, high energy consumption, and large secondary pollution.
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Embodiment 1
[0021] Preparation of each raw material cell
[0022] (1) Cultivation of protein-degrading bacteria
[0023] The protein-degrading bacteria used in this example are high-efficiency protein-degrading bacteria screened in the natural environment. They were identified as Aspergillus and Mucor by morphological and molecular identification. They were inoculated into 50ml liquid medium and cultured with shaking at 30°C for 24 hours, and then collected by centrifugation. bacteria.
[0024] The liquid medium used was soluble starch 2g, potassium nitrate 0.1g, dipotassium hydrogen phosphate trihydrate 0.05g, sodium chloride 0.05g, magnesium sulfate heptahydrate 0.05g, ferrous sulfate heptahydrate 0.001g, distilled water 100mL, pH7 .2-7.4, 0.1MPa sterilization for 20 minutes. Glucose 1g, peptone 0.5g, potassium dihydrogen phosphate trihydrate 0.1g, magnesium sulfate heptahydrate 0.05g, Bengal red (1mg / mL) 0.33mL, water 100mL, natural pH, 0.1MPa sterilization for 20 minutes, add 2% to ...
Embodiment 2
[0035] Preparation of microbial agents
[0036] Preparation of composite bacteria
[0037] According to the culture method of Example 1, each bacterial strain used for the degradation of kitchen waste is cultured separately, then the thalline is collected by centrifugation, and mixed according to the following ratio to prepare the composite thalline, Aspergillus oryzae: Candida lipolytica: Bacillus subtilis: Trichoderma konningen = 2:1:2:2.
[0038] Preparation of carrier material
[0039] Bamboo charcoal, sawdust, soil, zeolite, and rice bran are mixed according to the following mass proportions to make a carrier material, bamboo charcoal: wood chips: soil: zeolite: rice bran=1:1:2:2:2.
[0040] Preparation of composite microbial agent
[0041] The composite microbial bacterial agent is prepared by mixing the composite bacterium with the carrier material, and the composite bacterium accounts for 5% of the mass percentage of the carrier material.
Embodiment 3
[0043] Preparation of microbial agents
[0044] Preparation of composite bacteria
[0045] According to the culture method of Example 1, the various strains used for the degradation of kitchen waste are cultivated separately, and then the thalline is collected by centrifugation, and mixed according to the following ratio to prepare the composite thalline, Mucor actininomyces: Bacillus subtilis: Bacillus licheniformis : Coriolus versicolor=1:2:1:2.
[0046] Preparation of carrier material
[0047] Bamboo charcoal, sawdust, soil, zeolite, and rice bran are mixed according to the following mass proportions to make a carrier material, bamboo charcoal: wood chips: soil: zeolite: rice bran=1:1:4:2:3.
[0048] Preparation of composite microbial agent
[0049] The composite microbial bacterial agent is prepared by mixing the composite bacterium with the carrier material, and the composite bacterium accounts for 10% of the weight percentage of the carrier material.
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