Microbial bacterium agent for degrading Chinese kitchen refuses and preparing method thereof

A technology for microbial inoculants and kitchen waste, applied in the field of microorganisms, can solve the problems of less application, retention, and impact on compost quality, and achieve the effects of avoiding incompleteness, high energy consumption, and large secondary pollution.

Inactive Publication Date: 2012-07-18
田兴军 +1
View PDF1 Cites 32 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Research on the application of microbial flora to degrade food waste has been reported, but the actual application is seldom, and generally only stays at the laboratory level. At the same time, the deodorization technology in the biological treatment process is not perfect. The development of equipment and the research of corresponding processes Not mature enough, the impact of salt and oil in food waste on compost quality needs to be further resolved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microbial bacterium agent for degrading Chinese kitchen refuses and preparing method thereof
  • Microbial bacterium agent for degrading Chinese kitchen refuses and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Preparation of each raw material cell

[0022] (1) Cultivation of protein-degrading bacteria

[0023] The protein-degrading bacteria used in this example are high-efficiency protein-degrading bacteria screened in the natural environment. They were identified as Aspergillus and Mucor by morphological and molecular identification. They were inoculated into 50ml liquid medium and cultured with shaking at 30°C for 24 hours, and then collected by centrifugation. bacteria.

[0024] The liquid medium used was soluble starch 2g, potassium nitrate 0.1g, dipotassium hydrogen phosphate trihydrate 0.05g, sodium chloride 0.05g, magnesium sulfate heptahydrate 0.05g, ferrous sulfate heptahydrate 0.001g, distilled water 100mL, pH7 .2-7.4, 0.1MPa sterilization for 20 minutes. Glucose 1g, peptone 0.5g, potassium dihydrogen phosphate trihydrate 0.1g, magnesium sulfate heptahydrate 0.05g, Bengal red (1mg / mL) 0.33mL, water 100mL, natural pH, 0.1MPa sterilization for 20 minutes, add 2% to ...

Embodiment 2

[0035] Preparation of microbial agents

[0036] Preparation of composite bacteria

[0037] According to the culture method of Example 1, each bacterial strain used for the degradation of kitchen waste is cultured separately, then the thalline is collected by centrifugation, and mixed according to the following ratio to prepare the composite thalline, Aspergillus oryzae: Candida lipolytica: Bacillus subtilis: Trichoderma konningen = 2:1:2:2.

[0038] Preparation of carrier material

[0039] Bamboo charcoal, sawdust, soil, zeolite, and rice bran are mixed according to the following mass proportions to make a carrier material, bamboo charcoal: wood chips: soil: zeolite: rice bran=1:1:2:2:2.

[0040] Preparation of composite microbial agent

[0041] The composite microbial bacterial agent is prepared by mixing the composite bacterium with the carrier material, and the composite bacterium accounts for 5% of the mass percentage of the carrier material.

Embodiment 3

[0043] Preparation of microbial agents

[0044] Preparation of composite bacteria

[0045] According to the culture method of Example 1, the various strains used for the degradation of kitchen waste are cultivated separately, and then the thalline is collected by centrifugation, and mixed according to the following ratio to prepare the composite thalline, Mucor actininomyces: Bacillus subtilis: Bacillus licheniformis : Coriolus versicolor=1:2:1:2.

[0046] Preparation of carrier material

[0047] Bamboo charcoal, sawdust, soil, zeolite, and rice bran are mixed according to the following mass proportions to make a carrier material, bamboo charcoal: wood chips: soil: zeolite: rice bran=1:1:4:2:3.

[0048] Preparation of composite microbial agent

[0049] The composite microbial bacterial agent is prepared by mixing the composite bacterium with the carrier material, and the composite bacterium accounts for 10% of the weight percentage of the carrier material.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a microbial bacterium agent for degrading Chinese kitchen refuses, which contains the components by mass ratio: protein degrading bacteria: starch degrading bacteria: fat degrading bacteria: cellulose degrading bacteria= (1-2):(1-2): (1-2):(2-3). Simultaneously, the invention further discloses a preparing method of the microbial bacterium agent. Aiming at components of refuses in domestic kitchens, the corresponding microbial bacteria are adopted for gradual degrading, finally the purpose of completely degrading the refuses is achieved, not thorough degrading and long time consumption of the traditional land filling mode and large secondary pollution and high energy consumption of the burning mode are avoided, and the microbial bacterium agent for degrading Chinese kitchen refuses and the preparing method of the microbial bacterium agent can meet the present low carbon, environmental protection and energy saving requirements.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a microbial agent for degrading Chinese kitchen waste and a preparation method thereof. Background technique [0002] In the structure of urban domestic waste in my country, the proportion of kitchen waste is 30% to 50%, which is a relatively large proportion. The main components of food waste include protein, starch, dietary fiber, animal fat and other organic substances. It is characterized by high moisture content, high proportion of organic matter, high oil content, high salt content, etc. It is easy to rot and ferment and breed bacteria, and has the dual characteristics of typical resources and waste. At present, the main treatment methods of kitchen waste include incineration, landfill, feed and biological treatment technology. Among them, feed and biological treatment technologies are the mainstream processes for standardized treatment of kitchen waste at this s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/14C12N1/16C12N1/20B09B3/00C12R1/69C12R1/785C12R1/125C12R1/845C12R1/10C12R1/73C12R1/645C12R1/885
CPCY02W30/78
Inventor 田兴军蒋平于鑫
Owner 田兴军
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap