21 results about "Mucus layer" patented technology

This invention provides an in vitro cell culture comprising a cell monolayer containing mucus-producing cells and a mucus layer, as well as a method for preparing and using the same. [Solution] The method involves culturing mucus-producing cells on a cell support structure under conditions necessary to establish a mucus layer on the luminal side of a cell monolayer, thereby producing a living cell construct containing a cell monolayer with mucus-producing cells and a mucus layer. The mucus layer may be substantially impermeable to minute objects and may have a thickness of approximately 1 micron to 1 cm.

The application is a divisional application of 2025116336025, the invention provides an application of 2, 4-dihydroxy-6-methoxyacetophenone in preparation of a medicine for treating lacrimal gland secretion dysfunction related diseases, a lacrimal gland dysfunction dry eye mouse model is constructed in the application, and liposome entrapped 2, 4-dihydroxy-6-methoxyacetophenone is adopted for intervention, so that the medicine for treating lacrimal gland secretion dysfunction related diseases is obtained. Experimental results show that the ocular surface function and structure of a model animal are remarkably improved through treatment of 2, 4-dihydroxy-6-methoxyacetophenone, the cornea sodium fluorescein staining score of a mouse in a 2, 4-dihydroxy-6-methoxyacetophenone treatment group is remarkably reduced, and cornea epithelium injury is effectively repaired; the epithelial structure in the cornea tissue tends to be complete, and inflammatory cell infiltration of a matrix layer is obviously relieved; the density of goblet cells in the conjunctiva is obviously increased, which indicates that the secretion function of the mucous layer on the ocular surface is recovered. The treatment of 2, 4-dihydroxy-6-methoxyacetophenone has an improvement trend on tear film rupture time and tear secretion amount, which indicates that the 2, 4-dihydroxy-6-methoxyacetophenone has a potential effect in the aspects of maintaining tear film stability and promoting basic tear secretion.

The invention discloses a bionic gastric mucosa micro-barrier structure, comprising: a bottom microfluidic channel for simulating a submucosal vascular network; the middle gastric epithelial cell layer is used for simulating a mucous membrane epithelial barrier and comprises a matrix layer, quaternary functional cells, a culture medium and a pH buffer module; and the surface dynamic mucus layer is used for simulating a mucous membrane protective barrier and comprises a vagus neurotransmitter simulant, exogenous mucus and a pressure sensor. According to the invention, continuous nutrition supply is provided for the middle gastric epithelial cell layer through the bottom microfluidic channel, the survival and functions of cells are maintained, and accurate tracking of the transgastric mucosa transport process is realized. A function collaborative network is formed through quaternary cells, the secretion volume and the injury state are monitored in real time, regulatory factors are dynamically adjusted, and it is ensured that a cell layer can still maintain the barrier function stable under external interference. When foreign matter impact or food friction signals are recognized through the pressure sensor, the pump speed is instantly increased, and local mucus loss is rapidly supplemented.

The invention belongs to the technical field of agricultural science, and particularly relates to a tissue slice manufacturing method capable of measuring the thickness of a mucus layer of a pig intestinal tract. The operation process is simple, the damage to tissues is small, and the detection of the intestinal mucous layer of an animal with a larger enteric cavity can be realized. The core technology capable of observing the mucous layer is to ensure that chyme is not separated from the intestinal wall in the section manufacturing link, the traditional paraffin section technology is tedious in operation process and comprises dehydration, transparency and wax immersion, and the frozen section directly enters the section link, so that the separation of chyme and intestinal wall tissues caused by treatment such as ethanol gradient dehydration and high-temperature wax immersion is avoided, and the operation process is simplified. And the sample is not easy to cut in a slicing link, and a mucus layer is not easy to separate, break or lose after dyeing. The frozen section can directly freeze chyme and intestinal tissues without damaging a mucus layer between the chyme and the intestinal tissues, so that a complete mucus layer is obtained.

This presently disclosed subject matter relates to an in vitro cell culture comprising a cell monolayer comprising mucus producing cells and a mucus layer, and methods of making and using the same. The methods including culturing mucus producing cells on a cell support structure under conditions to establish a mucus layer on the luminal side of the cell monolayer, thereby producing a live cell construct comprising a cell monolayer comprising mucus producing cells and a mucus layer. The mucus layer can be substantially impenetrable to micro-objects, and have a thickness of about 1 micron to about 1 cm.

Ingestible devices can directly deliver therapeutic agents to desired tissue(s) of the GI tract of a subject, such as the submucosa, the mucosa, and / or the mucus layer of the GI tract, and methods of using the same. The ingestible devices can deliver therapeutic agents in a safe, effective, and reliable manner. The disclosure also provides pharmaceutical compositions for use in methods of treating a disease or condition in a subject in need thereof.

The invention belongs to the technical field of food processing, and discloses a use method of a phosphorus-free water-retaining agent suitable for silver carp and bighead carp heads. The method comprises the following steps: selectively depolymerizing a mucus layer on the skin of a fish head by using alkaline protease, trehalose and a sodium citrate pretreatment solution through a mucus layer nanopore channel induction-low-temperature vacuum tumbling synergistic permeation process to form 50-200nm through micropores; and then combining vacuum rolling and kneading at 4 DEG C to drive non-phosphorus water retention components such as sodium carboxymethyl cellulose and L-arginine to uniformly permeate into muscular tissues at a depth of more than 8mm. According to the method disclosed by the invention, a set of complete, controllable and efficient phosphorus-free water retention technical system is constructed through cooperative regulation and control of interface microstructure engineering and mass transfer kinetics, so that not only is the problem of core quality deterioration in deep processing of silver carp and bighead carp heads solved, but also a transferable technical normal form is provided for phosphorus-free preservation of other high-mucus covering type aquatic products.

This presently disclosed subject matter relates to an in vitro cell culture comprising a cell monolayer comprising mucus producing cells and a mucus layer, and methods of making and using the same. The methods including culturing mucus producing cells on a cell support structure under conditions to establish a mucus layer on the luminal side of the cell monolayer, thereby producing a live cell construct comprising a cell monolayer comprising mucus producing cells and a mucus layer. The mucus layer can be substantially impenetrable to micro-objects, and have a thickness of about 1 micron to about 1 cm.

The environment-friendly log board spliced by using the isinglass comprises two log panels, a right log base plate and a left log base plate are arranged between the two log panels respectively, a splicing connection protrusion is arranged on the left side face of the right log base plate, a splicing groove is formed in the right side face of the left log base plate, and the splicing connection protrusion is connected with the splicing groove. The left end of the splicing connection protrusion extends into the splicing groove, and a swimming bladder glue layer is arranged in the splicing groove. According to the wood floor, the splicing connection protrusions arranged on the right log base plate are matched with the splicing grooves in the left log base plate, the left ends of the splicing connection protrusions can be inserted into the splicing grooves, and the right log base plate and the left log base plate can be firmly spliced through the swimming bladder glue layer; and through cooperation of the first connecting protrusions, the second connecting protrusions, the first connecting grooves and the second connecting grooves, the log side panels and the log side plates can be firmly spliced on the side faces of the right log base plate and the left log base plate.

A method for translocating a cationic micro / nanoparticle across a mucus layer, the method including (a) contacting the mucus layer with at least one alginate oligomer, the alginate oligomer having at least 70% mannuronate residues, together with the cationic micro / nanoparticle; or (b)(i) contacting the cationic micro / nanoparticle with an alginate oligomer having at least 70% mannuronate residues thereby forming a micro / nanoparticle carrying the alginate oligomer, and (b)(ii) contacting the mucus layer with the micro / nanoparticle prepared in step (b)(i). There is also provided a method for translocating a molecule of interest across a mucus layer including contacting the mucus layer with a micro / nanoparticle which is formed of self-assembling micro / nanoparticle forming components, wherein at least one is a cationic micro / nanoparticle forming agent and at least one is an alginate oligomer which has at least 70% mannuronate residues, and further comprises the molecule of interest, optionally wherein the molecule of interest is covalently bound to one or more of the self-assembling micro / nanoparticle forming components.

The invention discloses nano magnesium oxide and a preparation method and application of an enteric-coated preparation of the nano magnesium oxide, and belongs to the technical field of feed additives. The preparation method comprises the following steps: preparing high-purity nano magnesium oxide powder through a hydrothermal precipitation-calcination technical route in combination with precise process parameter regulation and control, and coating the high-purity nano magnesium oxide through a coating technology to obtain the enteric-coated preparation. The pH response material adopted during coating has pH responsiveness, and the nano magnesium oxide is protected from being damaged by strong acidic conditions of gastric juice, so that the stability and the bioavailability of the nano magnesium oxide are improved; chitosan quaternary ammonium salt serves as a stabilizer and is good in biocompatibility and adhesiveness, positive charges carried by chitosan quaternary ammonium salt can interact with negative charges of an intestinal mucous layer, the retention time of nano magnesium oxide in the mucous layer can be prolonged, accurate release of magnesium ions in the intestinal tract is achieved, and the antibacterial performance of the nano magnesium oxide is improved.

The application discloses an active peptide for regulating intestinal epithelial mucus barrier function and a preparation method and application thereof, and belongs to the technical field of bioengineering, wherein the amino acid sequence of the active peptide is shown as SEQ ID NO: 1, 2, 3, 4, 5 or 6. The application takes duck skin as raw material, extracts a protein extract, carries out enzymolysis, collects low-molecular-weight peptide components through ultrafiltration grading, carries out freeze-drying to obtain active peptide crude powder, and then carries out screening and separation and purification on the active peptide crude powder through an intestinal epithelial mucus barrier damage model to obtain a target active peptide. The active peptide can significantly improve the secretion level of mucin, promote the expression of a MUC2 gene, maintain the secretion function of goblet cells, restore the integrity of a mucus layer, effectively improve the damaged intestinal epithelial mucus barrier function under the stimulation of inflammation and oxidation stress, and block the contact between pathogenic microorganisms and toxins and epithelial cells. The active peptide obtained by the application has clear structure and definite function, and provides a new way for high-value utilization of livestock and poultry by-products and development of intestinal health products.

Ingestible devices can deliver therapeutic agents directly to desired tissues of a subject's GI tract, such as the submucosa, mucosa, and / or mucus layer of the GI tract, and methods of using the same. Ingestible devices can deliver therapeutic agents in a safe, effective, and reliable manner. The present disclosure also provides pharmaceutical compositions for use in methods of treating a disease or condition in a subject in need thereof.

The invention discloses a semen euryales gel with an intestinal barrier repairing function and a preparation method of the semen euryales gel, and belongs to the field of medical biological materials. The preparation method comprises the following steps: drying and crushing Asiaocai fruits, carrying out ultrasonic extraction to obtain an Asiaocai extracting solution, preparing a composite solution from the Asiaocai extracting solution and additives such as sodium alginate or modified sodium alginate, finally dropwise adding the composite solution into a coagulating bath containing a divalent or trivalent metal ion cross-linking agent, and carrying out a cross-linking reaction to obtain the Asiaocai gel. According to the gel, a compact and stable three-dimensional network structure is constructed through the combined action of the semen euryales pectin and the modified polysaccharide, the gel has excellent mechanical strength, gastrointestinal tract degradation resistance and precise colon targeting performance, the intestinal barrier can be repaired through the dual mechanisms of reconstructing epithelial cell tight connection and repairing a mucous layer, and the intestinal tract targeting effect is improved. The symptoms of weight loss, colon shortening and the like of mice with acute colitis are remarkably improved, inflammatory cell infiltration is reduced, an efficient targeted delivery carrier is provided for treating inflammatory bowel diseases, and the application prospect is wide.

The present invention relates to ingestible devices for delivery of therapeutic agents to the gastrointestinal tract. The ingestible device may deliver a therapeutic agent directly to a desired tissue of the GI tract of a subject, such as a submucosal, mucosal, and / or mucous layer of the GI tract, and methods of using the same. The ingestible device can deliver the therapeutic agent in a safe, efficient and reliable manner. The disclosure also provides a pharmaceutical composition for use in a method of treating a disease or condition in a subject in need thereof.

The invention relates to a preparation method and application of bioactive lipid nanoparticles for autonomously and efficiently penetrating mucus barriers to enhance cystic fibrosis treatment, provides effective drugs for efficient CF inhalation treatment, and provides technical support for inhaling lipid nanoparticles to deliver nucleic acid drugs to efficiently penetrate lung barriers at the same time. The method comprises the following steps: constructing a bioactive nano-motor TBP-coated LNP-cmRNA with a self-driving characteristic, promoting a nano-system to efficiently penetrate through an airway mucus layer by using asymmetrically generated gas impetus of the bioactive nano-motor TBP-coated LNP-cmRNA, and delivering a nucleic acid drug CFTR mRNA to airway epithelial cells; meanwhile, H2S generated in the movement process of the nano system plays a role in relaxing bronchus and synergistically and efficiently treats CF, and when the nano system autonomously moves to cross the lung mucus barrier, the excellent H2S generation capability of the nano system is utilized to autonomously cross the mucus barrier, so that the CF treatment effect is greatly improved on the basis of autonomously cross the mucus barrier. Over-contracted airway smooth muscle cells in a pathological state are efficiently relaxed in situ, the trachea is obviously relaxed, and the airway blockage degree is relieved.

The invention relates to the technical field of surface plasma resonance, and discloses an establishment method and application of an intestinal wall cell mucous layer model. The method comprises the following steps: carrying out bionic treatment on the surface of a metasurface plasmon resonance chip, inputting a culture solution containing intestinal wall cells into the surface of the metasurface plasmon resonance chip through a micro-fluidic chip to carry out intestinal wall cell culture, inputting an intestinal tract simulation solution into the surface of the metasurface plasmon resonance chip, and carrying out intestinal tract simulation on the surface of the metasurface plasmon resonance chip. And detecting the refractive index change of the surface of the metasurface plasmon resonance chip, and monitoring the forming process of the mucus layer of the intestinal wall cell in real time according to the refractive index change. According to the intestinal wall cell mucous layer model obtained through the construction method, unmarked real-time continuous monitoring of the intestinal wall cell mucous layer forming process, dynamic observation of physiological changes of cells and simulation of the real intestinal environment can be achieved, and the method has high sensitivity and accuracy, is low in detection cost and has remarkable market prospects.

The invention relates to the technical field of rivet manufacturing, in particular to an anti-loose rivet which comprises a connecting cap, a core column, a protrusion and a baffle, caulking grooves are evenly formed in the inner side wall of the connecting cap at intervals, mucus layers are arranged in inner cavities of the caulking grooves, seepage holes used for being communicated with the inner cavities of the caulking grooves are formed in the outer wall of the connecting cap, and the mucus layers are arranged in the seepage holes. A plugging structure used for preventing the mucus layer from leaking out of the connecting cap without external force is arranged in an inner cavity of the seepage hole, an extrusion structure used for extruding the mucus layer out of the connecting cap is arranged at the end, close to the protrusion, of the caulking groove, the core column is pulled, so that the protrusion is extruded into the cavity of the connecting cap, the extrusion structure is triggered, and the mucus layer is extruded out of the connecting cap. Under the action of pressure, the mucus layer opens the plugging structure, so that the mucus layer flows out along the seepage holes. Through the scheme, the riveting stability is improved, and the phenomenon of riveting failure is reduced.

Ingestible devices can directly deliver therapeutic agents to desired tissue(s) of the GI tract of a subject, such as the submucosa, the mucosa, and / or the mucus layer of the GI tract, and methods of using the same. The ingestible devices can deliver therapeutic agents in a safe, effective, and reliable manner. The disclosure also provides pharmaceutical compositions for use in methods of treating a disease or condition in a subject in need thereof.

This invention relates to the field of aquatic life rescue technology, and discloses a rescue device for large aquatic organisms and its method of use. The large aquatic life rescue device provided by this invention features a flexible support surface and multiple interconnected inflatable chambers at the bottom, with a total buoyancy less than the fish's own weight. After the fish is placed, its own weight causes the flexible surface to passively collapse, adaptively forming an arc-shaped support surface that conforms to the three-dimensional contour of the abdomen, thus evenly distributing pressure and preventing tissue ischemia, necrosis, or damage to the mucus layer. The collapsed sidewalls can flexibly suppress displacement and movement of the fish during struggle, reducing stress response. By continuously adjusting the inflation volume, the same platform can accommodate large aquatic organisms of different weights and abdominal shapes, expanding the device's applicability.

This invention relates to the field of biotechnology, specifically to a gastric-targeted glycyrrhetinic acid self-assembled nanodelivery system, its preparation method, and its application in inhibiting Helicobacter pylori infection. The nanoassembly is formed by the self-assembly of a system comprising glycyrrhetinic acid and metal ions; wherein the metal ions and glycyrrhetinic acid self-assemble through non-covalent interactions to form a structure with a nanoscale morphology; the nanoscale morphology is selected from at least one of nanotubes, nanoribbons, and nanovesicles. The nanoassembly of this invention, constructed by the self-assembly of glycyrrhetinic acid driven by metal ions, can achieve long-term retention in the stomach (>24 h) and effectively penetrate the gastric mucus layer, thereby maintaining a high effective concentration at the site of gastric inflammation. Furthermore, when applied to the treatment of inflammatory diseases of the gastrointestinal tract, it can effectively inhibit and kill pathogenic bacteria without easily inducing bacterial resistance, maintaining the diversity of the gastrointestinal flora, and thus avoiding the dysbiosis caused by traditional antibiotic treatment, showing broad application prospects.