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Methods for preparing and detecting chyle particle migration model in mucus layer

A detection method and technology of mucus, applied in the direction of measuring device, transmittance measurement, scattering characteristic measurement, etc., to achieve the effect of simple preparation, good repeatability and good representativeness

Pending Publication Date: 2021-03-26
BOHAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies of the prior art, the present invention provides a preparation and detection method of a chylomicron migration model in the mucus layer, which solves the problem of simply and clearly studying the migration of chylosomes by simulating the mucus layer in vitro

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  • Methods for preparing and detecting chyle particle migration model in mucus layer
  • Methods for preparing and detecting chyle particle migration model in mucus layer

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preparation example Construction

[0031] Such as Figure 1-2 As shown, the present invention provides a technical solution: a preparation method of chylomicron migration model in the mucus layer, comprising the following steps:

[0032] S1. Obtain animal intestinal mucus;

[0033] S2, preparing artificial chyle and storing it under certain conditions;

[0034] S3, adding intestinal mucus into the sample bottle, and then adding artificial chyle.

[0035] In step S1, the intestinal mucus comes from the large intestine of the pig. The large intestine of the pig must be fresh. It is best to take the large intestine of the pig immediately after slaughter to extract the intestinal mucus. It can be stored on ice during transportation. Before extracting the intestinal mucus, use PBS buffer Gently wash the dirt on the surface of the large intestine of the pig, but do not rub it hard. After cleaning, cut the large intestine into small pieces of 15-20 cm, rub the surface of the large intestine vigorously to collect muc...

Embodiment 1

[0046] A preparation method of a chylomicron migration model in a mucus layer, comprising the following steps:

[0047] S1. Obtain animal intestinal mucus.

[0048] S2. Prepare artificial chyle and store it under certain conditions.

[0049]S3, adding intestinal mucus into the sample bottle, and then adding artificial chyle.

[0050] In step S1, the large intestine of the pig was made into a small section of 15 cm, and the intestinal mucus was extracted after washing the large intestine of the pig with PBS buffer solution (pH7.2-7.4): NaCl 8g / L, KCl 0.2g / L, NaCl 2 HPO 4 3.63g / L, KH 2 PO 4 0.24g / L, the volume ratio of protease inhibitor cocktail (100x, DMSO stock solution) to intestinal mucus was 1:100.

[0051] In step S2, first fully dissolve the substance to be detected according to the required concentration, mix it with simulated saliva at a volume ratio of 1:1, add salivary amylase, so that the enzyme activity value of salivary amylase is 70 U / mL, adjust When the pH...

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Abstract

The invention discloses methods for preparing and detecting a chyle particle migration model in a mucus layer, and relates to the technical field of preparation and detection of particle migration models, and the preparation method comprises the following steps: S1, obtaining animal intestinal mucus; S2, preparing artificial chyle, and storing the artificial chyle under a certain condition; s3, adding the intestinal mucus into a sample bottle, and then adding the artificial chyle. According to the preparation and detection methods of the chyle particle migration model in the mucus layer, the problems of long conventional animal experiment period, high operation difficulty, ethical morality and the like are solved.

Description

technical field [0001] The invention relates to the technical field of preparation and detection of a particle migration model, in particular to a preparation and detection method of a chyloparticle migration model in a mucus layer. Background technique [0002] The intestinal tract is an important digestive organ of the human body. Its surface is covered with a layer of mucus. Through the continuous secretion and renewal of mucus, the epithelial cells are effectively isolated and removed from harmful substances such as bacteria and viruses in the intestinal tract, thereby lubricating and protecting the intestinal mucosa. The role of protection. The mucus layer is an important component of the intestinal barrier. Chylomicrons play an important role in the digestion and absorption of food. Studying the migration of chylomicrons can reveal the absorption and transport process of substances in intestinal mucus and the interaction between chylomicrons and intestinal mucus duri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/47G01N21/59
CPCG01N21/47G01N21/59G01N2021/4709
Inventor 杨立娜雒明朔刘贺吴星会蔡文琪李丽朱力杰王胜男宋虹何余堂马涛
Owner BOHAI UNIV
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