40 results about "Protein chemistry" patented technology

The present invention concerns a novel means by which chemical preparations can be made. Reactions can be accelerated on special chips using microwave energy. The chips contain materials that efficiently absorb microwave energy causing chemical reaction rate increases. The invention is important in many small scale chemical transformations including those used in protein chemistry and in combinatorial chemistry.

The invention pertains to the field of textile fiber processing and chemical technology of protein, in particular to a whole silk fibroin protein composite and a preparation method thereof. The invention adopts inorganic salt solution to preprocess degummed silk of directional arrangement which is taken as a reinforced layer, and then adopts concentrated regenerated silk fibroin aqueous solution to cast the degummed silk to form film, thus obtaining a whole silk fibroin protein composite with high performance. The whole silk fibroin protein composite has further optimized mechanical property after being processed by alcoholic solvents. Both the substrate and fiber phase of the composite are originated from fibroin protein, with good biocompatibility, are easy adhesive for cells and are biodegradable. Therefore, the composite has extensive application prospect in the field of medicine.

The invention discloses a method for synthesizing an alpha-amino acid, and belongs to the fields of organic chemistry, medicinal chemistry and protein chemistry. The method uses sunlight or visible light as an energy source, is added with an electron donor, and uses carbon dioxide and an imine compound as raw materials for synthesizing the alpha-amino acid at normal temperature and normal pressure. The method adopts cheap and readily available raw materials, uses extremely mild reaction conditions, avoids expensive and dangerous high-pressure reaction devices by using a carbon dioxide gas at the normal pressure, can use clean and infinite sunlight as the energy source, and eliminates subsequent purification operations by automatically separating out products from a system in a solid form after completing the reaction and simply filtering to obtain a target product.

A new and versatile class of cyclic diazodicarboxamides that reacts efficiently and selectively with phenols and the phenolic side chain of tyrosine through an Ene-like reaction is reported. This mild aqueous tyrosine ligation reaction works over a broad pH range and expands the repertoire of aqueous chemistries available for small molecule, peptide, and protein modification. The tyrosine ligation reactions are shown to be compatible with the labeling of native enzymes and antibodies in buffered aqueous solution. This reaction provides a novel synthetic approach to bispecific antibodies. This reaction will find broad utility in peptide and protein chemistry and in the chemistry of phenol-containing compounds.

The invention discloses a method for separating copper proteome by using copper-chelated magnetic beads, and relates to the technical field of protein chemistry. The method comprises the following steps: taking a biological tissue or cell, after fully grinding with liquid nitrogen, adding protein extracting liquid, and after uniformly mixing, carrying out low-temperature centrifugation, so as to obtain a denatured protein solution; mixing the copper-chelated magnetic beads with the denatured protein solution, and incubating to obtain a magnetic bead protein mixed solution; adding a buffer solution 1 with the volume 1 time of that of the magnetic bead protein mixed solution into the magnetic bead protein mixed solution for suspension, pouring half of the supernate under the external magnetic field, and repeating for 4-5 times to obtain a mixed solution A; adding a buffer solution 2 with the volume 1 time of that of the mixed solution A into the mixed solution A, separating all the specific copper-binding proteins, namely, the copper proteome, and collecting the copper proteome under the external magnetic field. According to the method, the metalloproteome is separated under a denaturing condition by using magnetic bead-IDA, so that the procedures are greatly simplified, and the obtaining efficiency of metalloproteins is improved; a method suitable for separating the metalloproteome from the tissue or cell under the excess heavy metal treatment condition is established.

The invention relates to the fields of protein chemistry, biology and medicine. More specifically, it relates to the design and preparation of proteinmimics of members of the cystine-knot growth factor superfamily. Further, the invention relates to the use of these proteinmimics as a medicament or prophylactic agent. The invention provides proteinmimics of members of the cystine-knot growth factor superfamily, preferably for use in immunogenic and/or therapeutic compositions.

A catalyst-free hydrazone formation chemical modification method for polypeptides or proteins based on electron-deficient benzaldehydes is provided. The method includes a step shown in the description, wherein an electron-deficient benzaldehyde compound shown as a formula III, adopted as an electrophilic agent, and a polypeptide or protein shown as a formula II, adopted as a nucleophilic agent, are subjected to a hydrazone formation reaction under a condition of a water-phase buffer solution to obtain a hydrazone formation product having a structure shown as a general formula I. In the formulaIII, R is a substitute having various structures, such as methyl, a fluorescent molecular substitute and a medicine molecule substitute. The invention also provides the electron-deficient benzaldehydes shown as the formula III and polypeptides or proteins modified with the electron-deficient benzaldehydes and obtained by the method.

The invention discloses a drug small molecule-protein target reaction prediction method based on multi-dimensional information. The method comprises the following steps: (1), obtaining feature representation of a protein target, including an amino acid vector, a protein three-dimensional space structure feature and a protein chemical feature; (2), obtaining feature representations of the drug small molecules, including semantic features of the drug molecules, general network features of BERT and chemical features of the drug molecules; (3), fusing the features of protein targets and drug smallmolecules; (4), using the fused features as input of a classifier, and training the whole network in combination with labels in a training set. The fusion of three different types of features can greatly mine the potential features of drugs and proteins, and is convenient for more accurate DTI prediction. The method can improve the DTI prediction speed and accuracy at the same time, and is higherin practical value.

The invention belongs to the field of protein chemistry, and particularly relates to a separation and purification method of recombinant protein. The separation and purification method of recombinant protein comprises the following steps: centrifuging fermentation broth containing target recombinant protein to obtain wet thalli; carrying out primary purification treatment such as bacterium breaking, washing and renaturation; carrying out filtering through at least one tangential flow membrane, and carrying out enzyme digestion; and then, carrying out at least two times of reversed phase chromatography, depyrogenation, ultrafiltration, bottling and the like to obtain insulin glargine with the purity of 99.7%. The method can effectively remove endotoxin and reduce impurities such as polymers, and is suitable for large-scale industrial production of insulin glargine.

A beverage composition made with low-calorie, lactose-free skim milk in liquid or dry powder form. Amelanchier alnifolia extract having Abscisic Acid is added to the skim milk in a multi-step process that utilizes CO₂ to maintain the nutrient value of the extract. Crystalline fructose, natural flavorings, nutrients and carbonation are added to produce a flavorful beverage composition. A method to make a carbonated beverage composition with low-calorie, lactose-free skim milk and Amelanchier alnifolia extract includes a series of steps to preserve the nutrient value of the extract.

The invention relates to a chemical cross-linking agent for proteins and a preparation method thereof. The multifunctional cross-linking agent provided by the invention has excellent water-solubilityand stability, and high cross-linking efficiency, especially high cross-linking efficiency to arginine, and the multifunctional cross-linking agent is the first extensively-used specific cross-linkingagent for arginine. It is proved that the cross-linking agent has arginine selectivity; and cross-linking reactions of the multifunctional cross-linking agent with proteins of eight modes and oligomeric yeast protein compounds prove the validity of conjugation between the cross-linking agent and arginine. Combined chemical cross-linking and mass spectrometric technology is an effective and rapidly developing technology in structural chemistry and can be effectively applied to analysis of large active protein compounds without strict purification. Due to diversified structural characteristicsof the cross-linking agent, the cross-linking agent presents complementary reactivity, and arginine residues cross-linkable with the cross-linking agent are all specific.

An antibody that reacts with 2-O-desulfated acharan sulfate, a hybridoma that produces the antibody, a detection method and a detection kit to which the antibody is applied are disclosed. The antibody that reacts with 2-O-desulfated acharan sulfate can be produced by immunizing a mammal using as an antigen a substance obtained by chemically bonding a protein to 2-O-desulfated acharan sulfate.

The invention relates to a method for producing C-terminal amidated dibasic or polybasic peptides, consisting in reacting two peptides in the presence of trypsin biologically active enzymes and, if necessary, in purifying the thus obtainable compounds of formula (I) by means of protein chemistry.

A set of paramagnetic particles synthesized by co-precipitation methods wherein an alkaline hydroxide solution is mixed with a metal salt solution. The alkaline hydroxide features ammonium hydroxide, potassium hydroxide, sodium hydroxide, or mixtures thereof. The metal salt solution features at least one ferrous salt and at least one tetravalent metal salt selected from Group 4 elements of the Periodic Table. The concentration of the ferrous salt is equal to or greater than the concentration of the tetravalent metal salt. The paramagnetic particles may be used for bioprocessing via magnetic fields. Bioprocessing, for example, may include purifying, concentrating, or detecting biomolecules of interest (e.g., nucleic acids, carbohydrates, peptides, proteins, other organic molecules, cells, organelles, microorganisms, viruses, etc.), or other magnetic field-based processes common to applications in separation science, diagnostics, molecular biology, protein chemistry, and clinical practice.

The invention relates to a succinylated soybean protein isolate acidic beverage and a preparation method thereof. The invention belongs to the field of acidic protein beverages and preparation thereof. The invention aims to solve the technical problems that the soybean protein beverage is unstable and easy to flocculate under the acidic condition in the existing protein chemical modification method. The acidic beverage is prepared from succinylated soybean protein, white granulated sugar, an acidulant, a compound stabilizer, an emulsifier and water. The acidic beverage with good stability and dispersity is obtained through reasonable matching of all the components and strict regulation and control of process conditions, meanwhile, the stability under the acidic condition is further improved through the modification effect of succinylation, the preparation process is simple in process, no other chemical reagents are added, safety is high, operability is high, and the preparation method is suitable for industrial production. The method is suitable for large-scale food industrial production.

The invention discloses a method for separating specific metal-binding proteins by using protein denaturation, and relates to the technical field of protein chemistry. The method comprises the following steps: firstly, taking a biological tissue or cell, after fully grinding with liquid nitrogen, adding protein extracting liquid, and after uniformly mixing, carrying out low-temperature centrifugation, so as to obtain a denatured protein solution; replacing Ni in Ni-NTA agarose with Cu, and putting into a chromatographic column; incubating the denatured protein solution with Cu-NTA agarose, so as to ensure that the denatured proteins are fully combined with the Cu-NTA agarose; then under the low-temperature condition, injecting eluant 1, so as to renature the proteins and elute non-specific copper-binding proteins; injecting eluant 2, so as to separate specific copper-binding proteins. According to the method, the copper-binding proteins under the denaturing condition are separated by using agarose-NTA, so that the obtaining efficiency of the copper-binding proteins is greatly improved; a technology suitable for separating the specific metal-binding proteins from the biological tissue or cell under the excess heavy metal treatment condition is established.