The invention discloses a composition for relieving asthenopia of the adolescent and promoting memory and a preparation method of the composition for relieving asthenopia of the adolescent and promoting memory. The composition is prepared by the following components in certain parts by weight: lutein ester, lutein, blueberry powder, vitamin A, taurine, vitamin B1, vitamin B2, vitamin B3, vitamin B6, vitamin B12, vitamin C, calcium pantothenate and zinc gluconate. The composition can be prepared into various food categories, preferably into the food categories of beverage, dairy product, biscuit, candy product and special diet. The composition of the invention is scientific in raw material compatibility and is capable of supplementing nutritional elements needed for the eyes and the brain, relieving asthenopia of the adolescent and promoting memory.