67results about How to "Reduce the difficulty of sampling" patented technology

The invention provides a tumor mutational load detecting method, a device and a storage medium. The method comprises the steps of acquiring mutation frequency data of a single testing sample, whereinthe mutation frequency data comprise the site mutation frequency of a sample target area, comparing the site mutation frequency with a preset threshold for obtaining a mononucleotide mutation which ishigher than the threshold, and eliminating meaningless mutation in mononucleotide mutation for obtaining the number of effective mononucleotide mutations; acquiring Indel mutation frequency data of the single testing sample, wherein the Indel mutation frequency data comprises the Indel mutation frequency of the sample target area, comparing the Indel mutation frequency with a preset threshold forobtaining the number of the Indel mutations which are higher than the threshold; calculating the number of the tumor mutational load according to a number evaluation formula of the tumor mutational load, wherein the number estimation formula comprises the weight item of the number of the effective mononucleotide mutations and the weight item of the number of the Indel mutations. The method according to the invention accurately detects the TMB index of the lung cancer sample under a precondition of no dependence to a common database and a normal pairing comparison sample.

The invention relates to a detection method for uplink timeslot confliction in a passive optical network and an optical line terminal thereof. The method mentioned in the invention comprises: A, the optical line terminal (OLT) samples the electrical signal peak of an uplink timeslot; and B, the OLT judges whether the sampled electrical signal peak exceeds the normal electrical signal peak of the uplink timeslot, if it is, determines that the timeslot confliction occurs in the uplink timeslot, otherwise determines that the uplink timeslot is normal. The inventive optical line terminal comprises an optical transmitter, an optical receiver and a control unit, wherein the optical receiver comprises a detection unit which further comprises a photoelectric conversion unit and an electrical signal detection unit. The invention also relates to a detection device which comprises a peak detection unit, a sampling unit, a comparison unit and a storage unit. The invention can sample and detect the electrical signal after photoelectric conversion, so that the detection of uplink timeslot confliction is simple and easy to operate with low cost.

The invention discloses a vaginal secretion sampling device used for gynecological clinic. The vaginal secretion sampling device comprises a sampling sleeve, wherein arc-shaped expansion pieces whichare symmetrically arranged are rotationally hinged to the left end of the sampling sleeve, silica gel is arranged on the outer walls of the arc-shaped expansion pieces, the two arc-shaped expansion pieces are matched to form a cone shape, a movable suction pipe is movably arranged in the sampling sleeve, and the left end of the movable suction pipe is fixedly connected with a sampling collection head of a hollow structure. The vagina is expanded through the arc-shaped expansion pieces, secretions at the deep position in the vagina can be sampled easily, the trouble that the secretions at the deep position in the vagina cannot be directly sampled in the past is solved, a secretion sample is sucked through negative pressure, the operation is easy, convenient and rapid, the sampling difficulty is reduced, meanwhile, after sampling is completed, the sample is relatively sealed, so that the sample is prevented from being polluted by the outside to influence the accuracy of a detection result.

The invention provides an undisturbed soil electric sampling device and system. The device comprises a handle, a dowel steel with vertical grain and an auger drill bit which are connected in sequence, a sleeve, inversed wedged shape iron hoops, a pair of slant threaded rods and a ring knife drill bit; the sleeve is composed of a plurality of steel discs surrounding the periphery of a platform, the platform is connected to the top ends of the steel discs, and wedged structures are arranged on the inner sides of the steel discs; a screw thread is arranged on the upper end of each steel disc, and nuts which are located on the upper portions of second through holes are connected to the upper ends of the steel discs through the screw threads; the inversed wedged shape iron hoops are located in sleeve, and when the nuts are rotated to make the steel discs move upward relative to the sleeve, the inversed wedged shape iron hoops can be jointed with the wedged structures and push the wedged structures outward; the slant threaded rods are symmetrically meshed with the vertical grain of the dowel steel vertically, so that the slant threaded rods rotate, and the dowel steel can move in the vertical direction; a main body of an ultrasonic transducer can replace the auger drill bit and can be connected with the dowel steel detachably; the ring knife drill bit is connected to a vibration end of the ultrasonic transducer; the device further comprises a driving power source which is used for supplying power to the ultrasonic transducer.

The invention relates to a remotely-controlled underwater small-size organism collector and relates to the field of water quality monitoring equipment; the remotely-controlled underwater small-size organism collector comprises a carrier float, a collection bottle, a frustum mesh, and a remote control; the carrier float is provided with a pushing power source and also provided with a winch unit for winding and unwinding the frustum mesh; the outlet end of the frustum mesh is connected to an opening of the collection bottle through a connector; the inlet end of the frustum mesh is also provided with an expansion device for contracting and expanding a mesh opening; the inlet end of the frustum mesh is also provided with a plurality of monitors; the carrier float is also provided with a console; the console is at least provided with a monitoring data recording module, a control module, and a signal transceiving module; data is transmitted between the console and the remote control through the signal transceiving module; the control module controls working states of the pushing power source, the winch unit, the expansion device, the connector and the monitors through a control circuit.

The invention relates to a sampling device, in particular to an unconsolidated sediment sampling device capable of sealing a bottom. The device comprises a pressing cover, a third sampling barrel, a moving part, an outer sleeve, a buckle, a second sampling barrel, a first sampling barrel, a connecting bend pipe, driving levers and bottom sealing plates, wherein the pressing cover is in threaded connection with the upper end of the third sampling barrel; the upper end of the outer sleeve is in threaded connection with the third sampling barrel; the lower end of the third sampling barrel is in threaded connection with the upper end of the second sampling barrel; the lower end of the second sampling barrel is in threaded connection with the upper end of the first sampling barrel; one end of the connecting bend pipe is in threaded connection with the moving part; the other end of the connecting bend pipe is in threaded connection with the driving levers; six driving levers are arranged andare rotatably connected with the first sampling barrel; six bottom sealing plates are arranged. The unconsolidated sediment sampling device capable of sealing the bottom has the beneficial effects ofsimple structure, convenience in operation, capability of supporting the bottom part of a sediment sample through the six bottom sealing plates, and capability of effectively avoiding the problem ofsample loss during a lifting process of the whole device.

The invention discloses a method for manufacturing an annular test-piece from a cylindrical rock-soil material sample, comprising the following steps: 1. manufacturing a cube remodeling regular sample: predicating and determining the size of the cube remodeling regular sample, manufacturing a molding die having an inner cavity of cubic shape, and preparing asphalt mixture; die-filling: sealing andwrapping the cylindrical rock-soil material sample to be sampled in the asphalt mixture; and de-molding to obtain a cube remodeling regular sample composed by the cylindrical rock-soil material sample and the asphalt mixture wrapped outside; 2. sampling; and 3. stripping, the asphalt mixture which is heated, softened and wrapped outside the cylindrical rock-soil material sample to obtain the annular test-piece. The invention has simple and convenient steps, convenient operation and low sampling cost, can simply and conveniently realize the sampling process of manufacturing an annular test-piece from a cylindrical rock-soil (coal, natural soil) material sample.

The invention discloses a geological detection drilling machine for disaster prevention and control, and relates to the technical field of geological disaster prevention and control research. The geological detection drilling machine for disaster prevention and control comprises a bottom supporting plate, wherein the right side of the top of the bottom supporting plate is fixedly connected with afirst rotating piece; the top of the first rotating piece is rotatably connected with a rotating fixing plate through a rotating shaft; the end, away from the first rotating piece, of the rotating fixed plate is fixedly connected with a drilling machine case; the lower part of the right side of the outer wall of the drilling machine case is fixedly connected with the bottom of the rotating fixingplate through an auxiliary fixing rod; a second rotating piece is fixedly connected to the bottom of the rotating fixing plate and is located on the right side of the auxiliary fixing rod; and the bottom of the second rotating piece is rotatably connected with a hydraulic telescopic rod through the rotating shaft. According to the geological detection drilling machine for disaster prevention and control, the problems of complex equipment, high cost, insufficient foundation bearing capacity, difficult deep layer sampling and inconvenient operation of an existing geological detection drilling machine are solved, and the aims of ensuring that the equipment is fixed to the ground soil to prevent sliding and loosening and conveniently sampling deep layer soil are achieved.

The invention provides a focal plane measuring device and defect detection equipment. The focal plane measuring device comprises a focus measuring light source, a focus measuring marking plate, an optical assembly, an imaging unit, a focal plane compensation structure and a control unit; the focus measuring light source is used for providing a first illumination light beam which is obliquely incident, the focus measuring marking plate comprises at least one group of focus measurement patterns, each group of focus measurement patterns comprises at least three sub-marks, and after the first illumination light beam is projected to the focus measuring marking plate, the focus measurement patterns are projected to the surface of a test sample piece and generate a reflection light beam; the focal plane compensation structure is located on one side of the imaging plane of the imaging unit and used for changing the transmission path of the reflected light beam, so that the imaging plane of the reflected light beam converged by the optical assembly is located on the receiving plane of the imaging unit to form at least one group of signal images, and each group of signal images comprises at least three sub-patterns; and the control unit acquires the vertical height of the test sample piece according to the position of the at least one sub-pattern. According to the invention, coaxial measurement of the focus measurement point and the defect detection point is realized, and the precision of focal plane measurement is improved.

The invention discloses a multi-polymerase chain reaction (PCR) primer for identifying a clouded leopard. The multi-PCR primer comprises primer pairs 1 shown in SEQ ID No:1 and SEQ ID No:2, and primer pairs 2 shown in SEQ ID No:3 and SEQ ID No:4. The invention also discloses a method for identifying the clouded leopard by using multi-PCR. The method comprises the following steps: extracting total deoxyribonucleic acid (DNA) of a to-be-tested sample and carrying out PCR amplification on the total DNA; and carrying out agarose gel electrophoresis detection on the amplified product, wherein the multi-PCR primer is the multi-PCR primer, and judging the to-be-tested sample to be the sample from the clouded leopard if two stripes appear in a lane. The invention also discloses an application of the multi-PCR primer or identification method in identification of the clouded leopard. The target of simply, feasibly, economically and practically identifying the clouded leopard is achieved by designing two pairs of primers.

The invention relates to a preparation method for a graphene/titanium-based nanocomposite without interface reaction, and belongs to the technical field of preparation of metal-based composites. The method comprises the following steps that firstly, graphene powder and titanium-based powder are uniformly mixed by adopting an ultrasonic dispersion technology, and then high-pressure torsion severe plastic deformation is carried out on the mixed powder to obtain the graphene/titanium-based nanocomposite without the interface reaction. The method is based on high-pressure torsion severe plastic deformation at the room temperature condition, on one hand, graphene can be uniformly dispersed in a titanium matrix, submicron or nanocrystallization of the titanium matrix can be obtained, and on the other hand, the reaction of graphene and the titanium matrix can be greatly inhibited, so that the composite without the interface reaction is obtained, and the bottleneck that the interface reaction of the graphene reinforced titanium matrix composite is difficult to control is fundamentally solved; and the method is beneficial to improving the mechanical property of the graphene/titanium-based nanocomposite.

The invention discloses a surface character printing device which comprises a rack. The surface character printing device further comprises an ink box, a driver and a character printing assembly whichare arranged on the rack, the character printing assembly comprises a plurality of character printing units and character printing plates matched with the character printing units, the driver is arranged between the ink box and the character printing assembly and drives the character printing unit to move up and down to complete printing, a plurality of small holes are formed in each character printing plate and are arranged in a matrix manner, and the arrangement manner of the multiple character printing units corresponds to that of the small holes; the driver drives the character printing units to move up and down, the different character printing units move up and down at the same time, lattice diagrams of to-be-printed characters are formed in small hole matrixes in the character printing plates, the lattice diagrams of the to-be-printed characters form same lattice diagrams on the surface of a cable to be printed with characters through the character printing units, and after inkdevelops, character identification is formed on the surface of the cable. By means of the surface character printing device, the letter fastness and definition can be guaranteed, harmful gas emissionis avoided in the manufacturing process, universality is high, and maintenance is convenient and quick.

The invention discloses an adjustable head-backward-leaning nasopharyngeal swab collection and examination chair, which relates to the field of medical instruments and comprises an auxiliary seat, and an auxiliary plate is arranged at the upper end of the auxiliary seat. By arranging the adjusting screw, the hinge sliding sleeve, the fixing rod, the adjusting rod, the upper hinge seat, the auxiliary plate and the connecting shaft, the auxiliary plate and the auxiliary seat form a qualified detected curve, then a detected person sits on the auxiliary seat, and then the sitting posture of the detected person is adjusted through the back cushion and the waist cushion arranged on the auxiliary seat; furthermore, the head of a detected person can automatically adapt to the interior of the groove formed in the auxiliary plate, the sitting posture of the detected person can be easily adjusted to be appropriate, the specimen collection process of medical staff is standardized, the situation that the detection result of the detected person is affected by the incorrect specimen collection process is avoided, and the detection efficiency is improved. And furthermore, clinical missed diagnosis and misdiagnosis are avoided, and the risk of exposure infection of medical personnel and the sampling difficulty are reduced.

The invention discloses an anti-interference narrow-pulse over-voltage detection circuit. The narrow-pulse over-voltage detection circuit is characterized in that a comparator is connected with the output end of an error amplifier, and the inverting input end of the comparator is connected with a ramp signal; the error amplifier is connected with a second reference voltage, and the inverting input end of the error amplifier is connected with a feedback signal; the R end of a first RS latch is connected with the comparator, and the S end of the RS latch is connected with a clock signal; the R end of a second RS latch is connected with the output end of the comparator and the R end of the first RS latch, the S end of the second RS latch is connected with the output end of a high-speed comparator; the input end of a front-stage driving circuit is connected with the output end of the first RS latch; the front-stage driving circuit is used for driving the grid of an N type field-effect tube switching tube; the grid of the N type field-effect tube switching tube is connected with the output end of the front-stage driving circuit, and the drain of the switching tube is connected with the other end of an inductor; the input end of a delay circuit is connected with the grid of the N type field-effect tube switching tube; one input end of a synchronous counter is connected with the second RS latch, and the other input end of the synchronous counter is connected with the delay circuit; the synchronous counter is used for outputting a turn-off signal.

The invention relates to a full-automatic fog water sampler based on sensor control and a using method thereof, which belong to the technical field of environment detection. The sampler comprises a variable-speed fan, a steady-flow box body, a filter screen and an air duct, wherein the variable-speed fan is arranged at one end of the air duct, a network camera is arranged on the air duct on the upper side of the variable-speed fan, a visibility sensor is arranged on the network camera, the filter screen is arranged at the other end of the air duct, a catch basin is arranged at the lower side of the filter screen, an integrated control terminal is arranged on the upper side of the air duct, and the variable-speed fan, the network camera and the visibility sensor are all connected to the integrated control terminal. The sampler is applied to external field monitoring, the fog water sample of the whole fog event is automatically collected, the sampling difficulty of atmospheric environmental science workers and the difficulty of later data processing are reduced, and long-period automatic sampling is realized.

The invention discloses a fish fry collecting device capable of controlling the sampling depth. The fish fry collecting device includes telescopic rods, a rotation rod, rotation devices, rotation handles, joint connectors, a fixing device and a cone-shaped net; the telescopic rods include first telescopic rods and a second telescopic rod; the rotation device includes first rotation devices and a second rotation device; the rotation handles are arranged on the second telescopic rod; the second telescopic rod is fixed on a boat body through the fixing device; the second telescopic rod is connected to the rotation rod through the second rotation device, and the rotation rod can rotate or rotate around the second telescopic rod through the second rotation device; the upper ends of the first telescopic rods are fixed on the rotation rod through the first rotation devices; and the lower ends of the first telescopic rods are connected to the cone-shaped net through the joint connectors. The fish fry collecting device is simple in structure, is easy to operate, can improve the sampling accuracy, can lower the sampling difficulty, can save time and effort, and can reduce errors.

The invention provides a liquid refrigerant detection sampling device. The device comprises a sampling bottle with a liquid inlet and a liquid outlet, and the liquid inlet and the liquid outlet of the sampling bottle are respectively connected to a first regulating valve and a second regulating valve. In liquid refrigerant sampling, the first regulating valve and the second regulating valve are opened simultaneously, the first regulating valve is connected to a refrigerant pot valve, the refrigerant pot valve is opened, a liquid refrigerant is injected into the sampling bottle, after air in the sampling bottle is completely discharged, the second regulating valve is opened, and when the liquid refrigerant satisfies sampling requirements, the refrigerant pot valve and the first regulating valve are closed so that a sampling process is finished. After the sampling bottle is taken off from the refrigerant pot, the sampling bottle can be connected to a fluorine injection pipe and a moisture test sample introduction needle by the first regulating valve or the second regulating valve for cooperation so that through the first regulating valve and the second regulating valve, refrigerant flow can be controlled so that a test is convenient and liquid refrigerant sampling difficulty is reduced. The invention also provides a liquid refrigerant detection sampling method used by the device.

The invention relates to a detection method of nucleic acid in a micro sample. The detection method includes acquiring a composite capture body comprising a magnetic core and a shell covering the magnetic core, wherein the shell is coupled with an antibody which has specific affinity to specific epitope of cells or viruses in the sample; putting the sample in a container, and contacting the sample with the composite capture body to enable the composite capture body to combined with the cells or viruses; applying a magnetic field to the container to adsorb the composite capture body to the inner wall of the container, and removing waste liquid; adding rinsing liquid to wash the composite capture body to obtain a composite capture body-cell combination or composite capture body-virus combination, and removing waste liquid; amplifying and detecting the composite capture body-cell combination or composite capture body-virus combination. By the detection method, the problems of complexity in operation of biological element separation and detection and low specificity in the prior art are solved, and automation and high-throughput treatment of separation and detection processes are realized easily.

The invention discloses an umbrella rib type environmental object surface sampling rod which comprises a protective cap, a sleeve handle, an umbrella rib type cotton swab, a tube body and reagents, wherein the sleeve handle is nested to the upper end of the tube body; the sleeve handle comprises a reagent storage bin and a sleeve; a valve communicated with the interior of the tube body is arrangedat the bottom of the reagent storage bin; the tube body penetrates through the reagent storage bin and is fixedly connected with the inner wall at the top of the reagent storage bin; an upper umbrella hub ring is arranged at the bottom of the sleeve; a plurality of umbrella rib supporting rods are rotationally connected onto the upper umbrella hub ring; the umbrella rib type cotton swab is arranged in the sleeve in a sliding manner; the top of a main rod of the umbrella rib type cotton swab is connected with a spring; a lower upper umbrella hub ring is arranged at the bottom of the umbrella rib type cotton swab and rotationally connected with an umbrella rib body; the middle of the umbrella rib body is rotationally connected with the umbrella rib supporting rods; the umbrella rib body iscoated with sampling cotton; the reagents are stored in the reagent storage bin; the protective cap is arranged above the sleeve handle. According to the umbrella rib type environmental object surfacesampling rod disclosed by the invention, the sampling operation links can be effectively simplified, pollution factors are reduced, and the sampling rod is convenient to carry and preserve.