Kit for diagnosing laryngeal cancer, and special biomarker of kit

A kit, a technology for laryngeal cancer, applied in the determination/examination of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc. question

Pending Publication Date: 2020-07-07
北京创新乐土生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, laryngeal cancer is difficult to detect in the early stage. Once diagnosed, it is usually late, and the physiological functions such as breathing, swallowing, and vocalization will be seriously affected after surgery, resulting in damage to the throat function and seriously affecting the quality of life of the patient.

Method used

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  • Kit for diagnosing laryngeal cancer, and special biomarker of kit
  • Kit for diagnosing laryngeal cancer, and special biomarker of kit
  • Kit for diagnosing laryngeal cancer, and special biomarker of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Example 1. Discovery of "biomarkers for the diagnosis of laryngeal cancer"

[0081] 1. Obtaining of saliva samples

[0082] The 32 cancer patient saliva samples were provided by 32 patients who had been clinically identified as laryngeal cancer.

[0083] Saliva samples from 9 patients with vocal cord polyps were provided by 9 patients who had clinically identified vocal cord polyps.

[0084] Saliva samples of 29 healthy individuals were provided by 29 healthy volunteers.

[0085] 2. Saliva flora analysis of the saliva samples to be tested

[0086] The cancer group consisted of 32 laryngeal cancer patients. The vocal cord polyposis group consisted of 9 patients with vocal cord polyps. The healthy group consisted of 29 healthy volunteers.

[0087] Genomic DNA was extracted from the saliva samples to be tested (composed of 32 saliva samples from cancer patients, 9 saliva samples from patients with vocal cord polyps and 29 samples from healthy individuals), and then th...

Embodiment 2

[0104] Example 2. Verification of "biomarkers for the diagnosis of laryngeal cancer"

[0105] 1. Obtaining of saliva samples

[0106] Same as Step 1 in Example 1.

[0107] 2. Real-time fluorescence quantitative PCR method to detect the relative abundance of the microorganisms to be tested

[0108] The cancer group consisted of 32 laryngeal cancer patients. The vocal cord polyposis group consisted of 9 patients with vocal cord polyps. The healthy group consisted of 29 healthy volunteers.

[0109] 1. Extract the genomic DNA of the saliva samples to be tested (composed of 32 saliva samples from cancer patients, 9 saliva samples from patients with vocal cord polyps, and 29 healthy people) and use them as templates to detect the samples to be tested by real-time fluorescent quantitative PCR The abundance of the microorganisms to be tested (Pseudomonas, Coagulum, Bacteroides, Faecalibacterium, or Clostridium rumenus) in saliva samples was then averaged by group.

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Abstract

The invention discloses a kit for diagnosing laryngeal cancer, and a special biomarker of the kit. The biomarker is composed of Pseudomonas, Aggregatibacter, Bacteroides, Faecalibacterium and Ruminiclostridium. Experiments show that the detection of the abundance of the biomarker in saliva of a test subject can diagnose laryngeal cancer with relatively high accuracy. Saliva samples are small in sampling difficulty, cannot cause trauma, and have the advantages of being non-invasive, safe, and inexpensive. The kit and biomarker has important application values.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a kit for diagnosing laryngeal cancer and a special biomarker thereof. Background technique [0002] Laryngeal cancer is one of the malignant tumors with poor prognosis in head and neck cancer. The five-year overall survival rate is only 15%-65%, the five-year survival rate is about 60% in the early stage, and only 17-32% in the late stage, and it is aggressive Strong, high transfer rate, easy to relapse after surgery. However, laryngeal cancer is difficult to detect in the early stage. Once diagnosed, it is usually late, and postoperatively will seriously affect the patient's physiological functions such as breathing, swallowing, and vocalization, resulting in damage to the throat function and seriously affecting the patient's quality of life. Therefore, it is very important to find an effective early screening method for laryngeal cancer to prevent laryngeal cancer and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886
Inventor 王丽丽黄志刚郭伟郭盈尹高菲石太平刘如银
Owner 北京创新乐土生物科技有限公司
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