Tissue single cell constant-temperature grinder

Abstract

The utility model discloses a tissue single cell constant-temperature grinder which comprises a base, a test tube support is arranged on the base, at least one test tube groove used for placing test tubes is arranged on the test tube support, a top support is arranged above the test tube support, and grinding rods are arranged at the positions, above the test tube grooves, of the top support. The top support is provided with a driver used for driving the grinding rods, and the base is provided with a lifting mechanism used for driving the top support to ascend or descend, and is characterized in that a heating water tank is arranged on the base, the top of the heating water tank is provided with insertion grooves below the test tube grooves, the bottoms of the test tubes are located in the corresponding insertion grooves, and the test tubes are arranged in the insertion grooves. A temporary storage groove is formed in the position, close to the front side of the heating water tank, of the inserting groove. The utility model provides a tissue single cell constant-temperature grinder which can automatically grind tissue cells, so that the grinding stability is good, the efficiency is high, and meanwhile, the temperature in the tissue cell grinding process can be ensured.

Description

Technical Field

[0001] This utility model relates to the technical field of cell grinding equipment, specifically a tissue single-cell constant temperature grinding device. Background Technology

[0002] Tissue single-cell grinding refers to the process of obtaining single cells from tissue cells through grinding. In existing techniques, the procedure involves placing a cell filter membrane at the mouth of a test tube, placing the tissue on top of the membrane, and then the operator holding a grinding rod with the grinding head pressed against the tissue on the membrane. With slight pressure and slow rotation of the grinding rod, the tissue cells are ground into single cells. These single cells then pass through the membrane and fall into the test tube, thus completing the single-cell acquisition. Clearly, this process is entirely manual, and the applied force and grinding motion vary from person to person, resulting in poor grinding stability and low efficiency.

[0003] In addition, when using a handheld grinding rod to grind tissue cells on a cell filter membrane, the tissue cells are greatly affected by the external ambient temperature. When the external ambient temperature is low, it will damage the cell tissue and affect the results of subsequent processing and analysis. Utility Model Content

[0004] The present invention aims to at least partially solve one of the technical problems in the related art: to provide a tissue single-cell constant temperature grinder that can automatically grind tissue cells, resulting in good grinding stability and high efficiency, while ensuring the temperature during the tissue cell grinding process.

[0005] Therefore, one objective of this utility model is to provide a tissue single-cell isothermal grinder, comprising a base, a test tube holder on the base, at least one test tube slot for holding test tubes, a top support above the test tube holder, a grinding rod on the top support positioned above each test tube slot, and a driver on the top support for driving each grinding rod to rotate around its own axis; the base is provided with a lifting mechanism for driving the top support to rise or fall; the base is characterized by: a heating water tank on the base, a slot on the top of the heating water tank located below each test tube slot, the bottom of the test tube located in its corresponding slot, and a recessed temporary storage slot on the heating water tank near the front of the slot for placing empty test tubes or test tubes after grinding.

[0006] A lifting mechanism lowers the grinding rod, bringing its lower end against the tissue cells on the cell filter membrane. The actuator then drives the grinding rod to rotate steadily, automatically grinding the tissue cells. Single cells separated from the tissue cells pass through the cell filter membrane and fall into the test tube, eliminating the inconvenience of manual grinding. Furthermore, since the bottom of the test tube inserts into slots in a heating tank, these slots heat the test tube, maintaining a relatively ideal temperature throughout, thus ensuring the ground single cells fall into the test tube in a constant-temperature environment.

[0007] According to one example of the present invention, the opening of the temporary storage tank faces upward and is inclined in the front direction toward the location of the heating water tank.

[0008] According to one example of the present invention, the temporary storage slot is a long strip structure extending along the width direction.

[0009] According to one example of the present invention, the temporary storage tank has a plurality of positioning grooves on the inner side wall near the front of the heating water tank. The positioning grooves are arranged sequentially at intervals along the left and right directions, and the positioning grooves are set as arc-shaped structures that match the outer side wall of the test tube.

[0010] According to one example of the present invention, the temporary storage slot is a circular hole-shaped groove with a diameter matching the outer diameter of the test tube. There are multiple temporary storage slots, which are spaced apart along the left and right directions of the base.

[0011] According to one example of the present invention, the base has a support rod, and the test tube holder is mounted on the support rod and slides with the support rod so that the position of the test tube holder is adjustable along the height direction.

[0012] According to an example of this utility model, the upright is a hollow tubular structure, the top support includes a main rod and a horizontal plate arranged on the main rod, each grinding rod is installed on the horizontal plate, the upper end of the upright is sleeved on the main rod, and the lifting mechanism is an electric push rod, the two ends of the electric push rod are respectively connected to the upright and the main rod.

[0013] According to one example of the present invention, the horizontal plate has a plurality of connectors that are rotatably engaged with the horizontal plate, the driver is mounted on the horizontal plate and the output end of the driver is connected to each connector in a transmission manner, so that the driver can drive each connector to rotate, and the upper end of the grinding rod is detachably connected to the lower end of the corresponding connector.

[0014] According to one example of the present invention, the base is provided with a liquid supply device for adding wetting liquid to the cell filter membrane at the mouth of the test tube.

[0015] According to an example of the present invention, the liquid supply device includes a back plate disposed on a test tube support. The back plate is located horizontally on the rear side of the test tube groove. The back plate has a liquid supply chamber for containing wetting liquid inside. The back plate is provided with a liquid outlet at the position corresponding to each test tube opening. The liquid outlet is connected to the liquid supply chamber and is used to deliver wetting liquid onto the cell filter membrane.

[0016] The above technical solution has the following advantages or beneficial effects: First, the vertical position of the test tube holder is adjustable, allowing the bottom of the test tube to be inserted into the slot on the heating water tank, providing a grinding temperature higher than the constant temperature. Second, the added temporary storage slot can not only hold empty test tubes for easy replacement of the ground test tubes on the test tube holder, but also allow ground test tubes on the test tube holder to be temporarily stored. Furthermore, since the temporary storage slot is located on the heating water tank, it can continuously heat the test tubes, ensuring that the cells inside remain at a constant temperature. Third, the upper end of the grinding rod is detachably connected to the connector, facilitating the replacement of the grinding rod. Finally, the liquid supply device keeps the cell filter membrane wetted, which is beneficial for the smooth cell grinding process.

[0017] Additional aspects and advantages of this invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0018] Figure 1 This is a left-front view of the tissue single-cell thermostatic grinder of this utility model.

[0019] Figure 2 This is a right-rear view of the tissue single-cell thermostatic grinder of this utility model.

[0020] Figure 3 This is a front view schematic diagram of the tissue single-cell thermostatic grinder of this utility model.

[0021] Figure 4 for Figure 3 A cross-sectional view along the "AA" direction.

[0022] Figure 5 for Figure 4 A magnified view of a portion of region "B".

[0023] Figure 6 This is a top view schematic diagram of the tissue single-cell isothermal grinder of this utility model.

[0024] Figure 7 for Figure 6 A cross-sectional view along the "CC" direction.

[0025] Figure 8 for Figure 6 A cross-sectional view along the "DD" direction.

[0026] Figure 9 for Figure 7 A magnified view of the "E" region in the middle.

[0027] The components are as follows: 1. Base; 2. Upright pole; 3. Test tube holder; 4. Test tube trough; 5. Top support; 5.1. Main pole; 5.2. Horizontal plate; 5.3. Connector; 6. Lifting mechanism; 7. Grinding rod; 7.1. Grinding head; 8. Driver; 9. Heating water tank; 10. Slot; 11. Back plate; 12. Liquid supply chamber; 13. Liquid outlet; 14. Slit; 15. Water pump; 16. Water supply pipe; 17. Water return pipe; 18. Test tube; 19. Cell filter membrane; 20. Temporary storage tank; 20.1. Positioning groove; 21. Tube sleeve. Detailed Implementation

[0028] The embodiments of this utility model are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain this utility model, and should not be construed as limiting this utility model.

[0029] The tissue single-cell isothermal grinder according to an embodiment of the present invention will now be described in detail with reference to the accompanying drawings.

[0030] This invention provides a tissue single-cell isothermal grinder, as shown in the figure. It includes a base 1, a support rod 2, a test tube holder 3, a top support 5, grinding rods 7, and a driver 8. The support rod 2 stands upright on the base 1, and its lower end is fixed to the base 1. The test tube holder 3 is mounted on the support rod 2 and has multiple test tube slots 4 for placing test tubes 18, which are spaced horizontally. The top support 5 is located above the test tube holder 3, and the support rod 2 is provided with a lifting mechanism 6 for raising and lowering the top support 5. There are one or more grinding rods 7, which are mounted on the top support 5 and each grinding rod 7 is located above its corresponding test tube slot 4. The lower end of the grinding rod 7 has a grinding head 7.1 facing the corresponding test tube slot 4. When the grinding rod 7 moves down with the top support 5 to the working position, the grinding head 7.1 on the grinding rod 7 abuts against the cell filter membrane 19 on the corresponding test tube opening. The driver 8 is located on the top support 5 and is used to drive each grinding rod 7 to rotate around its own axis.

[0031] Preferably, there are six grinding rods 7.

[0032] The test tube support 3 in the above embodiment is along Figure 3The test tube support 3 has a long strip structure extending from left to right. Holes are drilled along the height direction to form a test tube groove 4. The outer diameter of the test tube 18 is equal to or slightly smaller than the inner diameter of the test tube groove 4. The width of the opening of the test tube 18 is greater than the inner diameter of the test tube groove 4, so that the test tube 18 can be stuck on the test tube groove 4 at the opening.

[0033] On the other hand, since test tubes 18 come in several different diameters when inserted into the test tube slot 4, in order to ensure that test tubes 18 of different diameters can be properly placed on the test tube slot 4, a preferred improvement to the test tube slot 4 on the test tube support 3 in the above embodiment is made as follows: Figure 9 As shown, the test tube groove 4 has a circular hole, and a detachable tube sleeve 21 is nested on the test tube groove 4. The upper end of the tube sleeve 21 has a flange that flares outward in the horizontal direction. The outer diameter of the tube sleeve 21 matches the inner diameter of the test tube groove 4, and the inner diameter of the tube sleeve 21 matches the outer diameter of the test tube 18. In this embodiment, by replacing tube sleeves 21 of different sizes, the inner diameter of different tube sleeves 21 can match test tubes 18 of different sizes.

[0034] During the process of grinding to obtain single cells, the temperature of the test tube 18 is affected by the external environment after the single cells fall into the test tube 18. Changes in the ambient temperature will affect the cells. In order to ensure that the single cells obtained after grinding the tissue cells can maintain a good biological state, the improvement of this embodiment is that: a heating water tank 9 is provided on the base 1, and a slot 10 is provided on the top of the heating water tank 9 below each test tube slot 4, and the bottom of the test tube 18 is located in the slot 10.

[0035] Furthermore, the heating water tank 9 has a recessed temporary storage groove 20 located on the slot 10 near the front side of the heating water tank 9, for placing empty test tubes 18 or test tubes 18 after grinding. In this embodiment, the front side of the heating water tank 9 in the front-back direction refers to the front-back direction, that is... Figure 3 In front of the vertical paper, the operator stands near the front of the heating water tank 9 for easy operation of the test tubes. The width of the temporary storage slot 20 is set to allow the body of the test tube 18 to be inserted into the temporary storage slot 20. The flared end of the test tube 18 is located outside the temporary storage slot 20. Thus, the temporary storage slot 20 can not only be used to place spare empty test tubes 18, but also to temporarily place the ground test tubes on the test tube support 3 into the temporary storage slot 20 after they have been ground, serving as a temporary resting place for the test tubes 18. During this process, the heating water tank 9 can continuously provide heat to maintain the temperature of the test tubes 18 when they are temporarily placed after being removed from the test tube support 3.

[0036] In a preferred embodiment of the above, the opening of the temporary storage tank 20 faces upward and is inclined toward the front side of the heating water tank 9 in the front-back direction.

[0037] Based on one of the preferred examples of the temporary storage slot 20 in the above embodiments: such as Figure 1 and Figure 6 As shown, the temporary storage slot 20 is a long strip structure extending along the width direction, that is, along... Figure 6 It extends in a long strip shape in the left and right directions.

[0038] To prevent collisions between test tubes, preferably, the temporary storage tank 20 has multiple positioning grooves 20.1 on its inner wall near the front of the heating water tank 9. These positioning grooves 20.1 are arranged sequentially at intervals along the left-right direction, and each positioning groove 20.1 is configured as an arc-shaped structure that matches the outer wall of the test tube 18. After the bottom of the test tube 18 is inserted into the temporary storage tank 20, the test tube 18 will be confined within the corresponding positioning groove 20.1, preventing the test tube 18 from moving in the left-right direction.

[0039] A second preferred embodiment of the temporary storage slot 20 described above: The temporary storage slot 20 is a circular groove with a diameter matching the outer diameter of the test tube 18. Multiple temporary storage slots 20 are spaced apart along the left-right direction of the base 1. A test tube 18 is inserted into a corresponding temporary storage slot 20, which can hold empty test tubes and also temporarily store test tubes that have been ground and have yielded single cells. During this process, the heating water tank 9 can heat the test tube 18 through the temporary storage slot 20, providing a constant temperature environment.

[0040] Preferably, the inner wall of the slot 10 in the heating water tank 9 can be thin-walled, so that the temperature of the heating water tank 9 can be better transferred to the slot 10 for heating the test tubes inside the slot 10.

[0041] The heating water tank 9 can be a hot water tank with an insulation layer on its outer wall, so that the hot water stored in the tank can continuously provide heat to the slot 10. The heating water tank 9 can also be a hot water tank with a heater. When the heater is powered on, it heats the water in the tank, and the resulting hot water maintains a constant temperature in the slot 10 through heat transfer.

[0042] Based on the improvement of the above embodiments: the test tube support 3 is slidably engaged with the upright 2 so that the position of the test tube support 3 along the height direction is adjustable.

[0043] Specifically, such as Figure 1 and Figure 7As shown, the test tube holder 3 has an assembly hole at the middle position along the left-right direction. This assembly hole fits over the upright 2. The test tube holder 3 is equipped with a locking component, which has an open state and a locked state. When the locking component is in the open state, the test tube holder 3 can be adjusted arbitrarily along the height direction. When the locking component is in the locked state, the locking component is fastened to the side wall of the upright 2, thus fixing the test tube holder 3 to the upright 2. Preferably, the locking component is a locking bolt.

[0044] like Figure 7 As shown, the upright 2 is a hollow tubular structure. The top support 5 includes a main rod 5.1 and a horizontal plate 5.2 arranged transversely on the main rod 5.1. The upper end of the main rod 5.1 is fixed at the middle position of the horizontal plate 5.2, and the lower end of the main rod 5.1 is inserted into the upper end of the upright 2, thereby connecting the upper end of the upright 2 and the lower end of the main rod 5.1. Each grinding rod 7 is installed on the horizontal plate 5.2 and aligned with its corresponding test tube groove 4. The lifting mechanism 6 is an electric actuator, with both ends connected to the upright 2 and the main rod 5.1, respectively. Thus, the extension and retraction of the electric actuator drives the main rod 5.1 to move up and down in the height direction. The base 1 is equipped with a controller that communicates with the electric actuator.

[0045] Based on the preferred embodiment described above, the horizontal plate 5.2 has a plurality of connectors 5.3 that rotate with the horizontal plate 5.2. The number of connectors 5.3, grinding rods 7, and test tube slots 4 are the same, and each connector 5.3, each grinding rod 7, and each test tube slot 4 corresponds one-to-one in the vertical direction. The driver 8 is mounted on the horizontal plate 5.2 and the output end of the driver 8 is connected to each connector 5.3 for transmission, so that the driver 8 can drive each connector 5.3 to rotate. The upper end of the grinding rod 7 is detachably connected to the lower end of the corresponding connector 5.3.

[0046] The lower end of the connector 5.3 is provided with a slot, the slot opening is set in the horizontal direction, and the slot matches the flange at the upper end of the grinding rod 7, so that the grinding rod 7 can be inserted into the slot in the horizontal direction.

[0047] Preferably, such as Figure 2 and Figure 7 As shown, the driver 8 includes a drive motor and a transmission belt. The drive motor is fixed inside the top bracket 5. The output shaft of the drive motor is provided with a drive wheel. The upper end of each connector 5.3 has a driven wheel. The drive wheel and each driven wheel are connected by a transmission belt.

[0048] Furthermore, the driver 8 includes multiple drive motors, each of which drives its corresponding connector 5.3 to rotate. It should be understood that, in order to reduce the number of drive motors, one or a few drive motors can be used to achieve the rotation of multiple connectors by switching the clutch of the transmission mechanism. This improvement is a conventional technical means in the prior art, and therefore, the transmission mechanism will not be described in detail in this embodiment.

[0049] During the grinding process of the grinding rod on the cell filter membrane 19, it is often necessary to manually add water or other wetting liquid. In order to reduce the inconvenience of manually adding wetting liquid, the improvement of this embodiment is that the base 1 is provided with a liquid supply device for adding wetting liquid to the cell filter membrane 19 at the mouth of the test tube 18.

[0050] Based on the preferred embodiment of the liquid supply device described above:

[0051] The liquid supply device includes a back plate 11 mounted on the test tube holder 3. The back plate 11 is located horizontally behind the test tube trough 4. The back plate 11 has a supply chamber 12 for containing wetting liquid. Each test tube 18 has an outlet 13 at its corresponding position on the back plate 11, which communicates with the supply chamber 12 and is used to deliver wetting liquid onto the cell filter membrane 19. In this embodiment, the test tube holder 3 is positioned along the front-back direction on the side where the person is standing (front side) and away from the person's standing position (rear side).

[0052] One preferred example: The back plate 11 is provided with multiple hoses, one end of each hose is connected to the corresponding liquid outlet 13, and the other end of the hose is located above the corresponding cell filter membrane 19 and is in contact with the cell filter membrane 19. Wetting liquid flows out through the tube opening of the hose to wet the cell filter membrane 19.

[0053] Second preferred example: Figure 5 As shown, the front side 11.1 of the back plate 11 has a slit 14, the cell filter membrane 19 is sheet-like, one side of the cell filter membrane 19 is clamped in the slit 14, and the liquid outlet 13 is arranged in the slit 14.

[0054] Based on the preferred embodiment of the above-mentioned liquid supply device, the liquid supply device includes a water pump 15 with a heating function. The outlet of the water pump 15 is connected to the water inlet on the back plate 11 through a water supply pipe 16. The water inlet on the back plate 11 is connected to the liquid supply chamber 12. The water pump 15 with the heating function is a conventional commercially available product in the prior art, and its structure and model will not be described in detail.

[0055] Preferably, the water pump 15 is a circulating water pump 15, which has a return water port. The return water port is connected to the return water interface on the back plate 11 through the return water pipe 17, and the return water interface on the back plate 11 is connected to the liquid supply chamber 12.

[0056] like Figure 2 The water inlet on the back plate 11 is located in the middle position, and the two ends of the back plate 11 are respectively provided with water return inlets. Hot water flows into the back plate 11 from the water inlet in the middle position and then flows back from the two ends.

[0057] In the above embodiments, the cell filter membrane is a porous sheet-like filter membrane, also known as a cell sieve.

[0058] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

[0059] For those skilled in the art, various changes and modifications will undoubtedly be apparent after reading the above description. Therefore, the appended claims should be considered as covering all changes and modifications that encompass the true intent and scope of this utility model. Any and all equivalent scope and content within the scope of the claims should be considered as still falling within the intent and scope of this utility model.

Claims

1. A tissue single-cell isothermal grinder, comprising a base (1), a test tube holder (3) on the base (1), at least one test tube slot (4) for holding test tubes (18) on the test tube holder (3), a top support (5) above the test tube holder (3), grinding rods (7) on the top support (5) located above each test tube slot (4), and a driver (8) on the top support (5) for driving each grinding rod (7) to rotate around its own axis, and a lifting mechanism (6) on the base for driving the top support (5) to rise or fall, characterized in that: The base (1) is provided with a heating water tank (9). The top of the heating water tank (9) is provided with a slot (10) below each test tube slot (4). The bottom of the test tube (18) is located in its corresponding slot (10). The heating water tank (9) has a recessed temporary storage slot (20) located near the front of the heating water tank (9) in the slot (10), which is used to place empty test tubes (18) or test tubes (18) after grinding.

2. The tissue single-cell isothermal grinder according to claim 1, characterized in that: The opening of the temporary storage tank (20) faces upward and is inclined towards the front side of the heating water tank (9) in the front-back direction.

3. The tissue single-cell isothermal grinder according to claim 2, characterized in that: The temporary storage slot (20) is a long strip structure extending along the width direction.

4. The tissue single-cell isothermal grinder according to claim 3, characterized in that: The temporary storage tank (20) has multiple positioning grooves (20.1) on the inner wall near the front of the heating water tank (9). Each positioning groove (20.1) is arranged sequentially and spaced apart in the left and right direction, and the positioning groove (20.1) is set as an arc-shaped structure that matches the outer wall of the test tube (18).

5. The tissue single-cell thermostatic grinder according to claim 2, characterized in that: The temporary storage slot (20) is a circular hole-shaped groove with a diameter matching the outer diameter of the test tube (18). There are multiple temporary storage slots (20), which are spaced apart along the left and right directions of the base (1).

6. The tissue single-cell isothermal grinder according to claim 2, characterized in that: The base (1) has a support rod (2), and the test tube holder (3) is installed on the support rod (2) and slides with the support rod (2) so that the position of the test tube holder (3) is adjustable along the height direction.

7. The tissue single-cell isothermal grinder according to claim 6, characterized in that: The upright (2) is a hollow tubular structure. The top support (5) includes a main rod (5.1) and a horizontal plate (5.2) arranged horizontally on the main rod (5.1). Each grinding rod (7) is installed on the horizontal plate (5.2). The upper end of the upright (2) is sleeved outside the main rod (5.1). The lifting mechanism (6) is an electric push rod. The two ends of the electric push rod are connected to the upright (2) and the main rod (5.1) respectively.

8. The tissue single-cell thermostatic grinder according to claim 7, characterized in that: The horizontal plate (5.2) has multiple connectors (5.3) that rotate with the horizontal plate (5.2). The driver (8) is mounted on the horizontal plate (5.2) and the output end of the driver (8) is connected to each connector (5.3) for transmission, so that the driver (8) can drive each connector (5.3) to rotate. The upper end of the grinding rod (7) is detachably connected to the lower end of the corresponding connector (5.3).

9. The tissue single-cell isothermal grinder according to any one of claims 1-8, characterized in that: The base (1) is equipped with a liquid supply device for adding wetting liquid to the cell filter membrane (19) at the opening of the test tube (18).

10. The tissue single-cell isothermal grinder according to claim 9, characterized in that: The liquid supply device includes a back plate (11) on the test tube support (3). The back plate (11) is located on the rear side of the test tube trough (4) in the horizontal direction. The back plate (11) has a liquid supply chamber (12) for containing wetting liquid. The back plate (11) is provided with a liquid outlet (13) at the position corresponding to the opening of each test tube (18). The liquid outlet (13) is connected to the liquid supply chamber (12) and is used to deliver wetting liquid to the cell filter membrane (19).

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