Thermotank for cell culture

Abstract

The utility model discloses a constant-temperature box for cell culture, which comprises a box body, a box body inner shell is fixedly connected to the side wall of the inner surface of the box body, a heating rod is arranged around the outer wall of the inner shell, a cell culture device is arranged at the lower end of the inner wall of the inner shell, the cell culture device comprises a motor, a tray and a disc, and positioning rods are arranged on two sides of the tray. Supporting plates corresponding to the positioning rods are arranged on the two sides of the inner shell, the trays comprise the first tray and the second tray, the first tray and the second tray are two semicircular plates, the first tray and the second tray are connected through a second limiting device, the output end of the motor is connected with a sleeve, and a spring is arranged in the sleeve. The upper end of the sleeve is rotatably connected with a guide rod, a fluctuating plate is arranged on the tray, the upper end of the guide rod is connected with a disc, and a plurality of test tube fixing tubes are arranged on the disc. The device has the beneficial effects that the problem of replacement of multi-specification trays is solved, and an extensible technical platform is provided for complex cell culture research.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell culture field, concretely relates to a thermostat for cell culture. BACKGROUND

[0002] The utility model discloses a thermostat for cell culture, and the cell culture tube is rotated and shaken while being moved up and down during cell culture, can make the cell in the cell culture tube distribute evenly without damaging the cell, also can make the nutrient substance in the cell culture solution distribute evenly in the cell culture tube, so that the cell can obtain suitable nutrition during the cell culture process, is favorable to the culture of cell.

[0003] The above-mentioned device cannot flexibly adjust the motion track of the cell bearing surface according to different experimental requirements, is difficult to simulate the dynamic microenvironment in the living body, and restricts the interaction efficiency of the cell and the culture solution. More importantly, the traditional structure needs to be operated as a whole during the replacement of the tray, and frequent disassembly not only increases the pollution risk, but also easily causes the wear of mechanical parts due to the rigid connection mode. UTILITY MODEL CONTENTS

[0004] The utility model aims at providing a thermostat for cell culture to solve the technical problem of difficult tray replacement mentioned above.

[0005] In order to solve the above technical problem, the utility model provides a thermostat for cell culture, which comprises a box body, a box body inner shell is fixedly connected to the inner surface side wall of the box body, a heating rod is arranged around the outer wall of the inner shell, a cell culture device is arranged at the lower end of the inner wall of the inner shell, the cell culture device comprises a motor, a tray and a disc, positioning rods are arranged on the two sides of the tray, support plates are arranged on the two sides of the inner shell corresponding to the positioning rods, the tray comprises a first tray and a second tray, the first tray and the second tray are two semicircular plates, the first tray and the second tray are connected through a second limiting device, the output end of the motor is connected with a sleeve, a spring is arranged in the sleeve, the upper end of the sleeve is rotationally connected with a guide rod, a heave plate is arranged on the tray, the upper end of the guide rod is connected with the disc, and a plurality of test tube fixing tubes are arranged on the disc.

[0006] Further, a plurality of interval equidistant air outlets are arranged through the upper end of the inner shell, the air outlets are connected with a centrifugal fan, and a plurality of interval equidistant air inlets are arranged through the lower end of the inner shell, the air inlets are connected with a negative pressure pump.

[0007] Further, a box door is arranged at the front end of the box body, a door frame is arranged around the box door, a first limiting device is arranged on the door frame, the first limiting device is provided with a first limiting rod, and a first limiting rod is arranged on the box door corresponding to the first limiting device.

[0008] Further, the rear end lower part of the first tray is provided with a second limiting device, and the second limiting device is provided with a second limiting rod, and the rear end lower part of the second tray is provided with a second limiting hole corresponding to the second limiting rod.

[0009] Further, one end of the spring is connected to the lower end of the sleeve, and the other end of the spring is connected to the upper end of the guide rod, and the sleeve and the guide rod are in sliding connection.

[0010] Further, the rear side of the box body is connected with a ventilation device.

[0011] Further, the lower end of the disc is fixedly installed with a plurality of guide columns in an annular array.

[0012] The cell culture incubator has the advantages that: compared with the prior art, the split electromagnetic locking tray structure can maintain the constant temperature environment in the box, and realize rapid adaptation and accurate regulation and control of the cell culture conditions. When the experimenters need to switch different culture parameters, they only need to release the locking state of the electromagnetic limiting device, and then manually slide the original semicircular tray along the box-shaped support plate out of the box, and then replace it with a new specification tray with preset curved parameters. The split tray adopts a standardized positioning interface, and the gap between the two positioning rods and the support plate is designed, so that different models of trays can automatically complete spatial calibration when sliding in, ensuring the continuity and synchronization of the curved surface track of the two semicircular trays after merging. By matching trays with different rise heights, the movement amplitude and frequency of the cell bearing surface can be flexibly adjusted, and the rapid switching of multiple dynamic culture modes in a single experimental period is realized, and the data comparison efficiency of high-throughput experiments is significantly improved. The multi-specification tray adaptation problem is solved, and an expandable technical platform is provided for complex cell culture research. BRIEF DESCRIPTION OF DRAWINGS

[0013] Figure 1 It is a schematic view of the three-dimensional structure of the box.

[0014] Figure 2 It is a schematic view of the three-dimensional structure of the box without a door frame.

[0015] Figure 3 It is a schematic view of the three-dimensional structure of the box without a door frame.

[0016] Figure 4 It is a schematic view of the three-dimensional structure of the inside of the shell.

[0017] Figure 5 It is a schematic view of the three-dimensional structure of the cell culture device without the first tray.

[0018] Figure 6 It is a schematic view of the three-dimensional structure of the cell culture device without the second tray.

[0019] Figure 7 The cell culture device is a schematic view of a bottom view structure.

[0020] Figure 8 The cell culture device is a schematic view of a longitudinal section structure of a bottom view.

[0021] Wherein: 1, box; 101, inner shell; 1010, support plate; 11, box door; 112, first limiting hole; 12, door frame; 121, first limiting device; 122, first limiting rod; 13, heating rod; 14, air exchange device; 2, air outlet; 21, centrifugal fan; 3, air inlet; 31, negative pressure pump; 4, cell culture device; 41, test tube fixing tube; 401, motor; 402, tray; 403, first tray; 410, second limiting hole; 42, second tray; 422, second limiting device; 423, second limiting rod; 431, guide rod; 432, spring; 433, sleeve; 434, undulating plate; 435, positioning rod; 436, disc; 4361, guide column. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present application will be described clearly and completely in the following description of the drawings in the embodiments of the present application. Obviously, the described embodiments are only one embodiment of the present application, rather than all the embodiments. Based on the embodiments in the present application, all the other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the present application.

[0023] In order to make the purpose, technical solutions and advantages of the present application more clear, the present application will be further described in detail in the following with reference to the drawings and specific embodiments.

[0024] In the following description, the reference to "one embodiment", "an embodiment", "one example", "an example" and the like indicates that the embodiment or example so described can include a particular feature, structure, characteristic, property, element or limitation, but every embodiment or example need not necessarily include the particular feature, structure, characteristic, property, element or limitation. Moreover, repeated use of the phrase "in one embodiment" does not necessarily refer to the same embodiment, although it can.

[0025] In the present embodiment: as shown in Figure 1 The box 1 is fixedly connected with the box 1 inner shell 101 on the inner surface side wall, which enhances the structural strength, isolates the internal and external environment, and reduces heat loss.

[0026] The outer wall of the inner shell 101 is provided with a heating rod 13, which is installed equidistantly on the outer wall of the inner shell 101. The heating rod 13 conducts heat through the inner shell 101 to achieve a uniform temperature field (fluctuation ≤±0.3℃) and avoid local overheating damage to cells.

[0027] The lower end of the inner wall of the inner shell 101 is provided with a cell culture device 4, which realizes automatic culture operation and supports high-throughput experiments. The cell culture device 4 includes a motor 401, a tray 402, and a disc 436. The two sides of the tray 402 are provided with positioning rods 435, and the two sides of the inner shell 101 are provided with support plates 1010 corresponding to the positioning rods 435. The opening direction of the support plate 1010 faces the box door 11, and the longitudinal section of the support plate 1010 is a box shape. The support plate 1010 is gap-fitted with the positioning rods 435. The tray 402 includes a first tray 403 and a second tray 42. The first tray 403 and the second tray 42 are two semicircular plates, which are modularized and split to facilitate replacement of different models of the tray 402. The height of the undulating plate 434 of the different models of the tray 402 is different to adapt to different culture conditions. A through hole is arranged in the middle of the tray 402, and the size of the through hole corresponding to the upper end of the guide rod 431 is adapted. The first tray 403 and the second tray 42 are connected through a second limiting device 422. Both the first limiting device 121 and the second limiting device 422 adopt an electromagnet device. The output end of the motor 401 is connected with a sleeve 433. A spring 432 is arranged in the sleeve 433. The upper end of the sleeve 433 is rotationally connected with the guide rod 431. An undulating plate 434 is arranged on the tray 402. The upper end of the guide rod 431 is connected with the disc 436. A plurality of test tube fixing tubes 41 are arranged on the disc 436.

[0028] A plurality of equidistant gas outlets 2 are arranged at the upper end of the inner shell 101. The gas outlets 2 are connected with a centrifugal fan 21. A plurality of equidistant air inlets 3 are arranged at the lower end of the inner shell 101. The air inlets 3 are connected with a negative pressure pump 31. When the experimenter opens the box door 11, the centrifugal fan 21 outputs hot air through the gas outlets, and the negative pressure pump 31 sucks air through the air inlets 3. The gas outlets 2 and the air inlets 3 are located at the front end of the inner shell 101 close to the box door 11. Air is sucked from the box through the centrifugal fan 21, is sprayed at high speed after being pressurized to form an air curtain, a negative pressure area is formed at the bottom of the opening, external air is actively sucked to form a dynamic isolation layer, the isolation effect of the air curtain is enhanced, the environment in the box is stabilized, a closed-loop air flow barrier is formed, and temperature / gas leakage is reduced.

[0029] The front end of the box body 1 is provided with a box door 11, the periphery of the box door 11 is provided with a door frame 12, the first limiting device 121 is arranged on the door frame 12, the first limiting device 121 is provided with the first limiting rod 122, the first limiting hole 112 corresponding to the first limiting device 121 is arranged on the box door 11, the opening and closing of the box door 11 is realized by controlling the extension and retraction of the first limiting rod 122 through the first limiting device 121, and the air tightness and anti-vibration interference of the box door 11 are ensured through the first limiting device 121.

[0030] The lower part of the rear end of the first tray 403 is provided with the second limiting device 422, the second limiting device 422 is provided with the second limiting rod 423, the lower part of the rear end of the second tray 42 is provided with the second limiting hole 410 corresponding to the second limiting rod 423, and the fixing of the first tray 403 and the second tray 42 is realized by controlling the extension and retraction of the second limiting rod 423 through the second limiting device 422.

[0031] One end of the spring 432 is connected to the lower end of the sleeve 433, the other end of the spring 432 is connected to the upper end of the guide rod 431, the sleeve 433 and the guide rod 431 are in sliding connection, the disc 436 is driven to rotate by the motor 401, the guide column 4361 below the disc 436 performs circular motion on the undulating plate 434, the disc 436 can realize regular up-down motion, the natural culture environment is simulated, and in addition, the contact area of cells and nutrient solution is improved, and the culture efficiency of cells is improved.

[0032] The air exchange device 14 is connected through the rear side of the box body 1, the air exchange device 14 is used for replacing air of the box body 1, adjusting the air pressure difference between inside and outside, and preventing gas from leaking in disorder.

[0033] Workflow: When the experimenter opens the door 11, the first limiting device 121 on the door frame 12 controls the first limiting rod 122 to retract to unlock; after the door 11 is pulled open, the centrifugal fan 21 at the upper end of the inner shell 101 of the box body 1 sprays high-speed airflow through the top air outlet 2, and the negative pressure pump 31 at the bottom sprays air through the air inlet 3, forming an air curtain barrier from top to bottom at the opening of the door 11. At this time, the heating rod 13 on the outer wall of the inner shell 101 of the box body 1 continuously conducts heat to maintain a constant temperature environment in the cell culture area. After the experimenter inserts the test tube containing the culture dish into the test tube fixing tube 41 of the disc 436, the cell culture device 4 is started: the motor 401 drives the sleeve 433 to rotate, the spring 432 inside the sleeve 433 and the guide rod 431 are elastically linked, so that the disc 436 at the top of the guide rod 431 moves along the curved surface track of the undulating plate 434 with the guide column 4361; the first tray 403 and the second tray 42 are slidingly connected with the box-shaped support plate 1010 on both sides of the inner shell 101 through the positioning rod 435, when the second limiting device 422 controls the second limiting rod 423 to insert into the corresponding second limiting hole 410, the two semicircular trays 402 are combined into a complete culture platform, and different cell culture conditions can be realized by replacing semicircular trays 402 of different sizes. The air exchange device 14 continuously adjusts the pressure difference between the inside and outside of the box body 1 through the connecting port at the rear side of the box body 1, when the door 11 is closed, the first limiting rod 122 reinserts into the first limiting hole 112 of the door frame 12 to make the box body 1 restore the closed state, and the centrifugal fan 21 and the negative pressure pump 31 enter the low-power standby mode.

[0034] The above description of disclosed embodiments enables a person skilled in the art to implement or use the present application. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the application. Therefore, the present application will not be limited to these embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A cell culture incubator, characterized in that: The device includes a housing, with an inner shell fixedly connected to the inner surface sidewall. A heating rod is arranged around the outer wall of the inner shell. A cell culture device is arranged at the lower end of the inner shell. The cell culture device includes a motor, a tray, and a disc. Positioning rods are arranged on both sides of the tray. Support plates are arranged on both sides of the inner shell corresponding to the positioning rods. The tray includes a first tray and a second tray, which are two semi-circular plates connected by a second limiting device. The output end of the motor is connected to a sleeve, and a spring is arranged inside the sleeve. A guide rod is rotatably connected to the upper end of the sleeve. An undulating plate is arranged on the tray, and the upper end of the guide rod is connected to the disc. Several test tube fixing tubes are arranged on the disc.

2. The cell culture incubator according to claim 1, characterized in that: The upper end of the inner shell is provided with several equally spaced air outlets, which are connected to a centrifugal fan. The lower end of the inner shell is provided with several equally spaced air inlets, which are connected to a negative pressure pump.

3. The cell culture incubator according to claim 1, characterized in that: The front end of the box is provided with a box door, and a door frame is provided around the box door. A first limiting device is provided on the door frame, and a first limiting rod is provided on the first limiting device. A first limiting rod is provided on the box door corresponding to the first limiting device.

4. The cell culture incubator according to claim 1, characterized in that: A second limiting device is provided at the lower rear end of the first tray, and a second limiting rod is provided on the second limiting device. A second limiting hole is provided at the lower rear end of the second tray corresponding to the second limiting rod.

5. A cell culture incubator according to claim 1, characterized in that: One end of the spring is connected to the lower end of the sleeve, and the other end of the spring is connected to the upper end of the guide rod. The sleeve and the guide rod are slidably connected.

6. The cell culture incubator according to claim 1, characterized in that: A ventilation device is connected through the rear side of the box.

7. The cell culture incubator according to claim 1, characterized in that: The lower end of the disk is fixedly equipped with a ring array of guide posts.

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