An antibacterial peptide separation and purification device

Abstract

The utility model discloses an antibacterial peptide separation and purification device relates to antibacterial peptide production and processing technical field, including separation and purification jar, the symmetry fixed connection of separation and purification jar outside wall has fixed seat, and is installed with the lifting assembly between fixed seat, and the top cover is installed with the top cover of lifting assembly to the top of separation and purification jar, and the bottom rotatory connection of top cover has the rotary column, and the rotary column outside wall fixed connection has centrifugal disc, and the top symmetry of centrifugal disc has placed the hole, and the inside bottom rotatory connection of separation and purification jar has the connecting column, and the bottom of rotary column is equipped with the slot, and the connecting column is inserted with the slot, and the slot bottom is equipped with the cross groove, and the connecting column top end fixed connection has the cross block, and the cross block is inserted with the cross groove. The utility model adopts above -mentioned structure, and the operation of installation and taking out without manual stoop and bend and stretch hand into the centrifuge inside, avoids the tedious step of repeatedly taking and placing and adjusting test tube in traditional manual operation, and the overall purification efficiency is improved significantly.

Description

Technical Field

[0001] This utility model belongs to the field of antimicrobial peptide production and processing technology, and specifically relates to an antimicrobial peptide separation and purification device. Background Technology

[0002] Antimicrobial peptide separation and purification devices are used to extract and purify peptides with antimicrobial activity from complex biological systems. Common devices include centrifuges, ultrafiltration units, and chromatography units. Centrifuges achieve solid-liquid separation through centrifugal force, removing impurities such as cell debris; ultrafiltration units utilize ultrafiltration membranes to retain target antimicrobial peptides according to molecular size, while removing small molecule impurities and concentrating the sample; chromatography units such as ion exchange chromatography, gel filtration chromatography, and affinity chromatography achieve fine separation based on charge difference, molecular size, and specific affinity, respectively.

[0003] Currently available centrifuges for the separation and purification of antimicrobial peptides require manual insertion of the arm into the centrifuge to load and unload test tubes. This necessitates bending or stooping close to the equipment, which is particularly problematic when the centrifuge is large or positioned low, limiting the operator's posture and leading to fatigue. Furthermore, the temperature inside the centrifuge rises after operation and remains residual heat for a short time after shutdown, increasing the risk of burns from manual insertion. Additionally, since antimicrobial peptide samples originate from biological materials containing pathogens, such as bacterial lysates, manual insertion can cause biocontamination if the test tubes are not properly sealed or leak. This increases the risk of infection for laboratory personnel, especially in environments without biosafety cabinets. Utility Model Content

[0004] In response to the problems mentioned in the background art, the purpose of this utility model is to provide an antimicrobial peptide separation and purification device to solve the problem that when experimental personnel manually reach into the centrifuge to load and unload test tubes, they need to bend over or lean close to the equipment. This is especially true when the centrifuge is large or placed in a low position, which restricts the operating posture and easily leads to limb fatigue. Moreover, when the centrifuge is running, the temperature of the chamber will rise, and residual heat will remain for a short time after the machine is stopped, which can easily cause burns when manually reaching in to remove the tubes.

[0005] The above-mentioned technical objective of this utility model is achieved through the following technical solution:

[0006] An antimicrobial peptide separation and purification device includes a separation and purification tank, fixed seats symmetrically and fixedly connected to the outer wall of the separation and purification tank, a lifting component installed between the fixed seats, a top cover installed on the top of the separation and purification tank via the lifting component, a rotating column rotatably connected to the bottom of the top cover, a centrifuge plate fixedly connected to the outer wall of the rotating column, placement holes symmetrically opened on the top of the centrifuge plate, and a connecting column rotatably connected to the bottom of the interior of the separation and purification tank.

[0007] The lifting assembly includes a second drive motor, a lead screw, a guide rod, a lifting seat, a connecting rod, and an assembly seat. The lead screw is rotatably connected to the fixed seats, and the guide rod is fixedly connected to each other. The second drive motor is installed at the bottom of the fixed seats, and its output end is fixedly connected to the lead screw. Lifting seats are fitted onto the outer sides of both the lead screw and the guide rod. Connecting rods are symmetrically fixedly connected to the top of the lifting seats. The other end of the connecting rod extends out of the top of the fixed seats and is fixedly connected to the assembly seat. The bottom of the assembly seat is fixedly connected to the top cover. The lead screw and the lifting seat are threaded together, while the guide rod and the lifting seat are slidably connected. This eliminates the need for manual bending and reaching into the centrifuge for installation and removal, avoiding the tedious steps of repeatedly picking up and adjusting test tubes in traditional manual operation. This significantly improves overall purification efficiency and prevents burns caused by residual heat inside the centrifuge, as well as mechanical injuries caused by the arm being caught in rotating parts when the centrifuge has not completely stopped operating.

[0008] As a preferred technical solution, the outer wall of the separation and purification tank is symmetrically provided with guide grooves, and a guide block is symmetrically fixedly connected to one side of the lifting seat. The guide block and the guide groove are slidably connected. The sliding cooperation between the guide block and the guide groove provides a clear motion trajectory for the up and down movement of the lifting seat, effectively avoiding shaking, deviation or tilting during the lifting process.

[0009] As a preferred technical solution, a connecting shaft is fixedly connected to the bottom of the connecting column, and the other end of the connecting shaft extends out of the bottom of the separation and purification tank. A first drive motor is installed at the bottom of the separation and purification tank. A drive gear is fixedly sleeved on the outer wall of the output shaft of the first drive motor, and a driven gear is fixedly sleeved on the outer wall of the connecting shaft. The drive gear and the driven gear mesh with each other, which can ensure a constant rotation speed and avoid unstable centrifugal force due to power fluctuations. This allows the antimicrobial peptide sample to be uniformly stressed during separation and purification, and impurities and antimicrobial peptides to be fully separated, effectively improving the separation and purification effect and efficiency.

[0010] As a preferred technical solution, a slot is provided at the bottom of the rotating column, and the connecting column is inserted into the slot. A cross groove is provided at the bottom of the slot. A cross block is fixedly connected to the top of the connecting column, and the cross block is inserted into the cross groove. A first limiting ring is fixedly sleeved on the outer wall of the rotating column, and a second limiting ring is fixedly sleeved on the outer wall of the connecting column. A limiting rod is symmetrically fixedly connected to the bottom of the first limiting ring, and a limiting hole is symmetrically opened at the top of the second limiting ring. The limiting rod and the limiting hole are slidably connected, which can form a multiple limiting and fixing mechanism, enhance the connection strength and reliability between the connecting column and the rotating column, and ensure the safe and stable operation of the centrifugation process.

[0011] As a preferred technical solution, a fixing ring is fixedly connected to the outer wall of the separation and purification vessel, and support legs are symmetrically fixedly connected to the bottom of the fixing ring. Anti-slip pads are glued to the bottom of the support legs. The anti-slip pads are made of soft rubber and have anti-slip textures on their surface, which greatly increases the friction between the bottom of the device and the surface on which it is placed. Even on a wet or smooth laboratory table, or when there is slight vibration due to centrifugation, the device can be effectively prevented from sliding, avoiding safety accidents such as collisions and tipping caused by device displacement, ensuring the safety of experimental personnel and equipment, and also preventing sample spillage and contamination caused by sliding.

[0012] In summary, the present invention has the following main advantages:

[0013] First, in this utility model, the test tube is inserted into the placement hole of the centrifuge plate, the second drive motor is started, and the lead screw is controlled to rotate. The lead screw and the lifting seat are threadedly driven, thereby controlling the lifting seat to drive the connecting rod to descend. The connecting rod drives the top cover to descend through the mounting seat. The top cover drives the rotating column, the centrifuge plate and the test tube to descend into the separation and purification tank. At the same time, the connecting column is inserted into the slot at the top of the rotating column and the cross block is inserted into the cross groove. There is no need for manual bending over to put hands into the centrifuge for installation and removal. This avoids the tedious steps of repeatedly taking out and adjusting test tubes in traditional manual operation, significantly improves the overall purification efficiency, and eliminates burns caused by residual heat inside the centrifuge, as well as mechanical injuries caused by the arm being caught in the rotating parts when the centrifuge has not completely stopped running.

[0014] Secondly, in this utility model, when the top cover drives the rotating column, centrifuge disc, and test tubes to descend into the separation and purification tank, and the connecting column is inserted into the slot at the top of the rotating column, and the cross block is inserted into the cross groove, the first drive motor is started, controlling the drive gear to drive the driven gear to rotate, thereby causing the connecting shaft to drive the connecting column to rotate. Because of the presence of the cross block, cross groove, limiting rod, and limiting hole, the connecting column drives the rotating column to rotate, which can keep the test tube rotating at a uniform speed, avoiding centrifugal force fluctuations caused by unstable transmission, thereby ensuring the uniformity of the antimicrobial peptide separation and purification effect, allowing impurities and antimicrobial peptides to be separated more fully and stably, and forming multiple limiting and fixing mechanisms, enhancing the connection strength and reliability between the connecting column and the rotating column, and ensuring the safe and stable operation of the centrifugation process. Attached Figure Description

[0015] Figure 1 This is a three-dimensional structural schematic diagram of the present invention;

[0016] Figure 2 This is a bottom-view three-dimensional structural diagram of the present invention;

[0017] Figure 3 This is a cross-sectional three-dimensional structural schematic diagram of the present invention;

[0018] Figure 4 This is the utility model Figure 3 Enlarged view of part A.

[0019] Reference numerals: 1. Separation and purification tank; 2. Fixing ring; 3. Support leg; 4. Anti-slip pad; 5. Top cover; 6. First drive motor; 7. Drive gear; 8. Connecting column; 9. Driven gear; 10. Rotating column; 11. Centrifuge disc; 12. Placement hole; 13. Slot; 14. Cross block; 15. Cross groove; 16. First limiting ring; 17. Second limiting ring; 18. Limiting rod; 19. Limiting hole; 20. Fixing seat; 21. Lifting assembly; 211. Second drive motor; 212. Lead screw; 213. Guide rod; 214. Lifting seat; 215. Connecting rod; 216. Assembly seat; 22. Guide groove; 23. Guide block; 24. Connecting shaft. Detailed Implementation

[0020] Example

[0021] refer to Figures 1 to 4 The antimicrobial peptide separation and purification device described in this embodiment includes a separation and purification tank 1. Fixed seats 20 are symmetrically fixedly connected to the outer wall of the separation and purification tank 1. A lifting assembly 21 is installed between the fixed seats 20. A top cover 5 is installed on the top of the separation and purification tank 1 through the lifting assembly 21. A rotating column 10 is rotatably connected to the bottom of the top cover 5. A centrifuge disc 11 is fixedly connected to the outer wall of the rotating column 10. Placement holes 12 are symmetrically opened on the top of the centrifuge disc 11. A connecting column 8 is rotatably connected to the bottom of the interior of the separation and purification tank 1.

[0022] The lifting assembly 21 includes a second drive motor 211, a lead screw 212, a guide rod 213, a lifting seat 214, a connecting rod 215, and an assembly seat 216. The lead screw 212 is rotatably connected to the fixed seats 20, and the guide rod 213 is fixedly connected to it. The second drive motor 211 is mounted on the bottom of the fixed seats 20, and its output end is fixedly connected to the lead screw 212. Lifting seats 214 are fitted onto the outer sides of both the lead screw 212 and the guide rod 213. Connecting rods 215 are symmetrically fixedly connected to the top of the lifting seats 214, and the other end of the connecting rod 215 extends out of the top of the fixed seats 20 and is fixedly connected to the assembly seat 216. The mounting base 216 is fixedly connected to the top cover 5 at its bottom. The lead screw 212 is threadedly connected to the lifting seat 214, and the guide rod 213 is slidably connected to the lifting seat 214. When the test tube is inserted into the placement hole 12 of the centrifuge plate 11, the second drive motor 211 is started to control the lead screw 212 to rotate. The lead screw 212 and the lifting seat 214 are threadedly driven, thereby controlling the lifting seat 214 to drive the connecting rod 215 to descend. The connecting rod 215 drives the top cover 5 to descend through the mounting base 216. The top cover 5 drives the rotating column 10, the centrifuge plate 11, and the test tube to descend into the separation and purification tank 1.

[0023] refer to Figure 2 The outer wall of the separation and purification tank 1 is symmetrically provided with guide grooves 22. The lifting seat 214 is symmetrically fixedly connected with guide blocks 23 on one side. The guide blocks 23 and the guide grooves 22 are slidably connected. When the lifting seat 214 is raised or lowered, the lifting seat 214 drives the guide blocks 23 to slide inside the guide grooves 22.

[0024] refer to Figure 2 A connecting shaft 24 is fixedly connected to the bottom of the connecting column 8. The other end of the connecting shaft 24 extends out of the bottom of the separation and purification tank 1. A first drive motor 6 is installed at the bottom of the separation and purification tank 1. A drive gear 7 is fixedly sleeved on the outer wall of the output shaft of the first drive motor 6. A driven gear 9 is fixedly sleeved on the outer wall of the connecting shaft 24. The drive gear 7 and the driven gear 9 mesh with each other. When the first drive motor 6 is started, the drive gear 7 is controlled to drive the driven gear 9 to rotate, thereby causing the connecting shaft 24 to drive the connecting column 8 to rotate. Due to the presence of the cross block 14, the cross groove 15, the limiting rod 18, and the limiting hole 19, the connecting column 8 drives the rotating column 10 to rotate. The rotating column 10 drives the centrifuge disc 11 and the test tubes on the centrifuge disc 11 to rotate.

[0025] refer to Figures 3 to 4 The rotating column 10 has a slot 13 at its bottom, and the connecting column 8 is inserted into the slot 13. The bottom of the slot 13 has a cross groove 15. The top of the connecting column 8 is fixedly connected to a cross block 14, which is inserted into the cross groove 15. When the top cover 5 drives the rotating column 10, the centrifuge plate 11, and the test tube to descend into the separation and purification tank 1, the connecting column 8 is inserted into the slot 13 at the top of the rotating column 10, and the cross block 14 is inserted into the cross groove 15.

[0026] refer to Figures 3 to 4 A first limiting ring 16 is fixedly sleeved on the outer wall of the rotating column 10, and a second limiting ring 17 is fixedly sleeved on the outer wall of the connecting column 8. A limiting rod 18 is symmetrically fixedly connected to the bottom of the first limiting ring 16, and a limiting hole 19 is symmetrically opened on the top of the second limiting ring 17. The limiting rod 18 and the limiting hole 19 are slidably connected. When the top cover 5 is controlled to move the rotating column 10 up and down by the lifting assembly 21, the rotating column 10 moves the first limiting ring 16 up and down, and the first limiting ring 16 moves the limiting rod 18 to slide inside the limiting hole 19.

[0027] refer to Figure 1 A fixing ring 2 is fixedly connected to the outer wall of the separation and purification tank 1. Support legs 3 are symmetrically fixedly connected to the bottom of the fixing ring 2. Anti-slip pads 4 are glued to the bottom of the support legs 3. The anti-slip pads 4 are made of soft rubber and have anti-slip textures on their surface. The anti-slip pads 4 greatly increase the friction between the bottom of the device and the placement surface.

[0028] Operating principle and advantages: First, insert the test tube into the placement hole 12 of the centrifuge plate 11, start the second drive motor 211, control the lead screw 212 to rotate, the lead screw 212 and the lifting seat 214 are threadedly driven, thereby controlling the lifting seat 214 to drive the connecting rod 215 to descend, the connecting rod 215 drives the top cover 5 to descend through the mounting seat 216, the top cover 5 drives the rotating column 10, the centrifuge plate 11 and the test tube to descend into the separation and purification tank 1, at the same time the connecting column 8 is inserted into the slot 13 at the top of the rotating column 10, the cross block 14 is inserted into the cross groove 15, start the first drive motor 6, control the drive gear 7 to drive the driven gear 9 to rotate, thereby causing the connecting shaft 24 to drive the connecting column 8 to rotate, the connecting column 8 drives the rotating column 10 to rotate due to the presence of the cross block 14, the cross groove 15 and the limiting rod 18 and the limiting hole 19, the connecting column 8 drives the rotating column 10 to rotate, the rotating column 10 drives the centrifuge plate 11 and the test tube on the centrifuge plate 11 to rotate;

[0029] This invention eliminates the need for manual bending over and reaching into the centrifuge to install and remove equipment, avoiding the tedious steps of repeatedly taking out and adjusting test tubes in traditional manual operation. It significantly improves the overall purification efficiency and also prevents burns caused by residual heat inside the centrifuge, as well as mechanical injuries caused by the arm being caught in the rotating parts when the centrifuge has not completely stopped running.

Claims

1. An antimicrobial peptide separation and purification device, comprising a separation and purification tank (1), characterized in that: The separation and purification tank (1) is symmetrically fixedly connected to the outer wall of the fixed base (20), and a lifting assembly (21) is installed between the fixed bases (20). The top of the separation and purification tank (1) is installed with a top cover (5) through the lifting assembly (21). The bottom of the top cover (5) is rotatably connected to a rotating column (10). The outer wall of the rotating column (10) is fixedly connected to a centrifuge disc (11). The top of the centrifuge disc (11) is symmetrically provided with placement holes (12). The bottom of the separation and purification tank (1) is rotatably connected to a connecting column (8). The lifting assembly (21) includes a second drive motor (211), a lead screw (212), a guide rod (213), a lifting seat (214), a connecting rod (215), and an assembly seat (216). The fixed seats (20) are respectively rotatably connected to the lead screw (212) and fixedly connected to the guide rod (213). The bottom of the fixed seat (20) is equipped with the second drive motor (211). The output end of the second drive motor (211) is fixedly connected to the lead screw (212). The lead screw (212) and the guide rod (213) are both fitted with lifting seats (214) on their outer sides. The top of the lifting seat (214) is symmetrically fixedly connected to the connecting rod (215). The other end of the connecting rod (215) extends out of the top of the fixed seat (20) and is fixedly connected to the assembly seat (216). The bottom of the assembly seat (216) is fixedly connected to the top cover (5).

2. The antimicrobial peptide separation and purification device according to claim 1, characterized in that: The lead screw (212) is threadedly connected to the lifting seat (214), and the guide rod (213) is slidably connected to the lifting seat (214).

3. The antimicrobial peptide separation and purification device according to claim 1, characterized in that: The outer wall of the separation and purification tank (1) is symmetrically provided with guide grooves (22), and the lifting seat (214) is symmetrically fixedly connected with guide blocks (23) on one side. The guide blocks (23) and guide grooves (22) are slidably connected.

4. The antimicrobial peptide separation and purification device according to claim 1, characterized in that: The bottom end of the connecting column (8) is fixedly connected to the connecting shaft (24), and the other end of the connecting shaft (24) extends out of the bottom of the separation and purification tank (1). The bottom of the separation and purification tank (1) is equipped with a first drive motor (6). The outer wall of the output shaft of the first drive motor (6) is fixedly fitted with a drive gear (7), and the outer wall of the connecting shaft (24) is fixedly fitted with a driven gear (9). The drive gear (7) and the driven gear (9) mesh with each other.

5. The antimicrobial peptide separation and purification device according to claim 1, characterized in that: The rotating column (10) has a slot (13) at its bottom. The connecting column (8) is inserted into the slot (13). The slot (13) has a cross groove (15) at its bottom. The connecting column (8) has a cross block (14) fixedly connected to its top. The cross block (14) is inserted into the cross groove (15).

6. The antimicrobial peptide separation and purification device according to claim 1, characterized in that: The outer wall of the rotating column (10) is fixedly fitted with a first limiting ring (16), and the outer wall of the connecting column (8) is fixedly fitted with a second limiting ring (17). The bottom of the first limiting ring (16) is symmetrically fixedly connected with a limiting rod (18), and the top of the second limiting ring (17) is symmetrically opened with limiting holes (19). The limiting rod (18) and the limiting hole (19) are slidably connected.

7. The antimicrobial peptide separation and purification device according to claim 1, characterized in that: The outer wall of the separation and purification tank (1) is fixedly connected to a fixing ring (2), and the bottom of the fixing ring (2) is symmetrically fixedly connected to a support leg (3). The bottom of the support leg (3) is glued with an anti-slip pad (4). The anti-slip pad (4) is made of soft rubber and has anti-slip texture on its surface.

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