Breeding method for brachymystax lenok triploid fingerling

A technology of triploid and leptinus salmon, which is applied in the field of raising seedlings of triploid salmon, can solve the problems of production benefit decline, seed quality degradation, and lag in basic theoretical research, and achieve low cost and good market Value, easy-to-operate effect

Inactive Publication Date: 2018-03-20
北京市水生野生动植物救护中心
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AI Technical Summary

Problems solved by technology

Salmon trout is currently the main cold-water cultured fish in my country. With the rapid development of salmon trout farming in China, the basic theoretical research is relatively lagging behind, and the degradation of broodstock germplasm leads to the degradation of seed quality and the decline in production efficiency. Restricting the sustainable development of salmon and trout farming (Sun Dajiang et al. Journal of Aquaculture, 2010, 23(2):56-63)

Method used

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Experimental program
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Embodiment 1

[0037] A preparation method for triploid salmon seedlings, specifically comprising the steps of:

[0038] 1) Egg collection and fertilization: choose 4 + Broodstock older than 500g, with strong physique and well-developed body, the size of male broodstock is over 500g, and the size of female broodstock is over 750g. The dose of oxytocic agent for female fish is 2.5ugLHRH-A per kilogram of fish 2 +2.5mgDOM, the male fish is halved, the ratio of male to female is 1:1, and the fertilization is done by dry method. The water temperature for egg collection is 6-8°C. Stir the sperm and eggs with a feather for 15-20s to mix the sperm and eggs evenly, add a small amount of water and stir slowly for 1 minute, then rinse with water for 2-3 times to wash away excess semen, egg skin, and blood clots, add water and let the eggs absorb water and swell.

[0039] 2) Heat shock induction: 15-20 minutes after fertilization, put the fertilized eggs into a stainless steel mesh with a diameter o...

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PUM

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Abstract

The invention relates to a breeding method for brachymystax lenok triploid fingerling. The breeding method comprises the following steps: in 15 to 20 min after fertilization, placing fertilized ova into a water bath at 17 to 20 DEG C, and carrying out transition for 1 min; then carrying out heat treatment in a water bath at 26 DEG C for 10 to 20 min; and carrying out incubation in an incubator with an incubation water temperature of 6 to 10 DEG C. The brachymystax lenok triploid fingerling can be obtained in about 30 days through the method provided by the invention. According to the invention, the fertilized ova reach an eyed rate of 80% or above and an incubation rate of 90% or above in an incubation period; and flow cytometer detection results show that a triploid rate of the fingerlingobtained by using the method provided by the invention reaches 100%. The breeding method for the brachymystax lenok triploid fingerling provided by the invention alleviates problems like high mortality of parent brachymystax lenok after propagation, slow growth speed and low disease resistance of young brachymystax lenok and low survival rate of juvenile brachymystax lenok in mouth-opening acclimation, has advantages like low cost and easy operation, and has good market value.

Description

technical field [0001] The invention relates to a method for raising seedlings of triploid salmon. Background technique [0002] Salmon trout is one of the main farmed fishes in the world at present. Due to its own quality and special requirements for the living environment, it determines the nature of its green, environmentally friendly and nutritious food, so it is very popular among consumers. According to FAO statistics, in recent years, the annual output of global salmon and trout farming has exceeded 2 million tons, ranking second to carps and tilapias, and is the third largest farmed fish in the world (Ji Feng et al. Journal of Aquaculture, 2012 , 25(3):63-68). Salmon trout is currently the main cold-water cultured fish in my country. With the rapid development of salmon trout farming in China, the basic theoretical research is relatively lagging behind, and the degradation of broodstock germplasm leads to the degradation of seed quality and the decline in production...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A01K61/17
CPCA01K61/10A01K61/17Y02A40/81
Inventor 陈春山魏凯郑伟胡红霞张保莉时晓韩姝伊董颖郭明磊马龙
Owner 北京市水生野生动植物救护中心
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