Artificial reproduction method for Triplophysa yarkandensis
A Yeerqiang plateau loach, artificial reproduction technology, applied in fish farming, application, climate change adaptation and other directions, can solve the problems of long effect time, low fertilization rate, low hatching rate, etc., to improve the fertilization rate and shorten the effect time. , the effect of increasing egg production
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[0012] 1. Experimental materials:
[0013] Select the Yarkand plateau loach that has been raised temporarily for 90 days, choose female fish over 3 to 5 years old, and male fish over 2 to 4 years old;
[0014] Choose chorionic gonadotropin (HCG), luteinizing hormone-releasing hormone analog (LRH-A 2 );
[0015] Prepare dry towels, several 1-5ml disposable syringes, 0.9% saline, etc.;
[0016] Equipped with water for oxytocin and hatching, using fully aerated tap water for standby, the water quality conditions are water temperature between 15-22°C, floating range 0.5°C, pH between 6.5-8.5, dissolved oxygen maintained above 4.0mg / L;
[0017] Before induced labor, prepare 2 running pools: length × width × height 2 × 0.6 × 0.3m, small cages: length × width × height 0.6 × 0.4 × 0.3m.
[0018] 2. Experimental method:
[0019] (1) Prenatal preparations: Before inducing labor, first use 20mg / L potassium permanganate to disinfect two flowing pools and a net cage for standby use. P...
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