A preparing method of a perinereis aibuhitensis anticoagulation peptide

A technology of double-toothed nereis and anticoagulant peptides, which is applied in the field of preparation of active peptides, and achieves the effects of good safety, high purity, and good medical application value

Active Publication Date: 2017-05-10
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Extracting anticoagulant components from clam worms by enzy...

Method used

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  • A preparing method of a perinereis aibuhitensis anticoagulation peptide
  • A preparing method of a perinereis aibuhitensis anticoagulation peptide
  • A preparing method of a perinereis aibuhitensis anticoagulation peptide

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Experimental program
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Embodiment

[0038] 1 Materials and methods

[0039] 1.1 Animals, materials and reagents

[0040] Healthy male clean-grade ICR mice, body weight (20±2) g; healthy male clean-grade SD rats, body weight (140±10) g, purchased from Zhejiang Academy of Medical Sciences, animal production license number: SCXK (Zhejiang )2014-0001.

[0041] The clam worm was purchased from Zhujiajian, Zhoushan, Zhejiang, and was identified by Professor Zhao Shenglong from Zhejiang Ocean University.

[0042] Trypsin, alkaline protease, neutral protease, papain, pepsin China Asia-Pacific Hengxin Company; fibrinogen, thrombin, carrageenan, ADP American Sigma Company; aspirin enteric-coated tablets Bayer Healthcare Co., Ltd.; others Reagents were all domestic analytically pure.

[0043] 1.2 Instruments and equipment

[0044] Cogent u Scale ultrafiltration system Merck Millipore; ApurifierUPC100 fast protein liquid chromatography system GE Healthcare; ALPHA1-4 freeze dryer Christ company of Germany; Agilent-1260 h...

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Abstract

A preparing method of a perinereis aibuhitensis anticoagulation peptide is disclosed. The method includes steps of (1) sample pretreatment, (2) enzymatic hydrolysis, (3) ultrafiltration, (4) anion exchange chromatography, (5) dextran gel G-25 chromatography, and (6) reversed-phase high-performance liquid chromatography separation and purification to finally obtain the anticoagulation peptide. The method has characteristics of high safety, mild reaction conditions and easy process control. The prepared anticoagulation peptide has high purity, high anticoagulation activity, an antithrombotic function and good medical application value.

Description

technical field [0001] The invention relates to a preparation method of an active peptide, in particular to a preparation method of an anticoagulant peptide of P. didentata. Background technique [0002] Thrombosis is the common pathological basis of ischemic heart disease, ischemic stroke and venous thrombosis, which seriously endangers human health and has been increasing in recent years. Inhibition of platelet aggregation and release, inhibition of thrombin system, activation of antithrombin and plasmin system can inhibit thrombus formation. The formation of thrombus is the main pathological process of ischemic cardiovascular and cerebrovascular diseases and thromboembolic diseases, and is closely related to the occurrence and development of diseases. Therefore, inhibiting platelet aggregation and thrombus formation is of great significance to the prevention and treatment of cardiovascular and cerebrovascular diseases and other thromboembolic diseases. [0003] Perinere...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/20C07K1/18C07K1/16
CPCC07K1/16C07K1/18C07K1/20C07K1/34C07K1/36C12P21/06
Inventor 丁国芳郑媛媛杨最素贾盈露余方苗徐银峰黄芳芳唐云平
Owner ZHEJIANG OCEAN UNIV
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