Arthrobacter and application thereof

A technology of Arthrobacter genus and bacteria, applied in Arthrobacter YC-RL1 and its application field, can solve the problem of low dissolved oxygen and achieve good economic value and application prospect

Active Publication Date: 2015-09-30
中国农业科学院研究生院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are relatively few studies on the biodegradation of pollutants in industrial wastewater by microorganism...

Method used

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  • Arthrobacter and application thereof
  • Arthrobacter and application thereof
  • Arthrobacter and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The isolation and identification of embodiment 1 Arthrobacter YC-RL1

[0031] 1. Isolation of strains

[0032] Activated sludge samples were collected at the outfall of Shandong Huayang Pesticide Factory (Tai'an City, Shandong Province). Under aseptic conditions, 5g of activated sludge samples were inoculated into 100mL of inorganic salt ion medium containing 50mg / L fluorene, and cultured at 30°C and 180rpm. After every 7 days of culture, transfer 10% of the culture volume to fresh inorganic salt medium, and increase the concentration of fluorene by 100mg / L each time, and transfer 5 times continuously until the concentration of fluorene in the inorganic salt medium increases to 300mg / L.

[0033] Gradiently dilute the acclimatized bacterial solution by 10 with sterile water -5 、10 -6 、10 -7 Afterwards, they were respectively spread on inorganic salt medium plates containing 100 mg / L fluorene, and cultured in a 30° C. incubator for 7 days. Pick a single colony on t...

Embodiment 2

[0042] The degradation performance test of embodiment 2 joint bacillus YC-RL1

[0043] 1. Degradation of naphthalene, fluorene, biphenyl, 1,2,3,4-tetrachlorobenzene and p-nitrophenol by Arthrobacter YC-RL1

[0044] Simultaneous degradation of naphthalene, fluorene, biphenyl, 1,2,3,4-tetrachlorobenzene and p-nitrophenol in inorganic salt medium by Arthrobacter YC-RL1 detected by high performance liquid chromatography , biphenyl, 1,2,3,4-tetrachlorobenzene and p-nitrophenol concentration tolerance.

[0045] Inoculate strain YC-RL1 into liquid LB medium for activation, and cultivate to logarithmic growth phase OD 600 =0.8, according to the inoculum size of 10% by volume, inoculate in the inorganic salt medium containing naphthalene, fluorene, biphenyl, 1,2,3,4-tetrachlorobenzene and p-nitrophenol each 100mg / L mixture simultaneously, As a treatment group, the inorganic salt medium containing naphthalene, fluorene, biphenyl, 1,2,3,4-tetrachlorobenzene and 100 mg / L mixture of p-ni...

Embodiment 3

[0059] Application of embodiment 3 Arthrobacter YC-RL1 in soil remediation

[0060] The soil used in this study was obtained from the Ximen Garden soil of the Chinese Academy of Agricultural Sciences. Arthrobacter YC-RL1 was cultured in LB liquid medium to the logarithmic phase (OD600=0.8, the cell concentration was about 2×10 8 CFU / mL), adding the prepared bacterial solution to the soil to a final concentration of 1×10 4 , 5×10 4 , 1×10 5 , 5×10 5 , 1×10 6 and 5×10 6 CFU / g soil (each processing is 100g soil), and adding naphthalene, fluorene, biphenyl, 1,2,3,4-tetrachlorobenzene and p-nitrophenol to the respective concentration is 100mg / kg respectively in the soil, As a treatment group; at the same time, the soil with the same concentration of pollutants and no bacteria was added under the same conditions as a control group, and the finally obtained culture system (treatment group and control group) was fully mixed. The samples were cultured in a constant temperature a...

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Abstract

The invention provides an arthrobacter and an application thereof. The arthrobacter is YC-RL1 collected with the serial number of CGMCC No.10611 and can be used for simultaneously degrading a mixture composed of 100mg/L naphthalene, 100mg/L fluorene, 100mg/L biphenol, 100mg/L 1, 2, 3, 4-tetrachlorobenzene and 100mg/L p-nitrophenol in an inorganic salt within 4 days. The arthrobacter has a relatively-wide tolerance range for salt ion concentration and can be used for simultaneously degrading a mixture composed of five 100mg/L compounds in an inorganic salt ion culture medium with the NaCl concentration of 0-60g/L within 5 days. YC-RL1 can be applied to biological remediation of an environment polluted by naphthalene, fluorene, biphenol, 1, 2, 3, 4-tetrachlorobenzene and p-nitrophenol and an environment simultaneously polluted by various pollutants, also has a favorable application potential for high-salt industrial production wastewater treatment of the pollutants and also has a relatively-high economic value and a relatively-good application prospect.

Description

technical field [0001] The invention relates to the field of microorganisms and biodegradation, in particular to an Arthrobacter sp. YC-RL1 and its application. Background technique [0002] The rapid development of industry and agriculture has led to a variety of pollutants entering the environment. Common organic pollutants include polycyclic aromatic hydrocarbons, such as naphthalene, fluorene, phenanthrene, anthracene, etc.; benzene and phenols, such as toluene, p-xylene, biphenyl, phenol, p-nitrophenol, etc.; halogenated substances, such as hexachloropropane , Triclosan, 1,2,3,4-tetrachlorobenzene, etc. The diversity of pollutants in the environment greatly increases the difficulty of remediation of polluted environments. Therefore, how to achieve efficient degradation of multiple pollutants at the same time has become an urgent problem to be solved. [0003] Naphthalene and fluorene are two relatively common polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydr...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/06C02F101/32C02F101/34C02F101/36C02F101/38
CPCB09C1/10C02F3/34C02F2101/32C02F2101/34C02F2101/36C02F2101/38C12N1/205C12R2001/06
Inventor 闫艳春任磊史延华贾阳
Owner 中国农业科学院研究生院
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