Biological agent prepared by Arthrobacter AS-1 strain and its application

Abstract

The present invention relates to biological preparation prepared with arthrobacter AS-1 strain and its application, and belongs to the field of microbial technology. The microbial strain is preserved in the preservation number of CGMCC No. 1281. The biological preparation is prepared through the process including the steps of: test seed culture, shaker culture, seeding tank culture and liquid fermentation in fermenting tank. The culture process has strain inoculating amount of 0.5-2 %, adopts the culture medium comprising beef paste, yeast extractum, peptone and glucose and of pH 6.8-7.2. The fermentation is controlled in 28-30 deg.c and pH 7.0-7.2, the fermentation time in the seeding tank is 16-20 hr, the fermentation time in the fermenting tank is 24-48 hr, and the biological preparation is obtained after the bacteria number in the fermentation liquid reaches 7.0 E8 / ml. The present invention has the advantages of short fermentation period, saving in power, low cost, no pollution, etc.

Description

Technical field: [0001] The invention relates to a biological preparation prepared by Arthrobacter AS-1 strain and application thereof, belonging to the technical field of microorganisms. Background technique: [0002] Tobacco is a kind of hobby product that people smoke, and nicotine volatilizes during the combustion process to make the smoke alkaline and produce pungent smell, which has a negative effect on the quality of tobacco leaves. The level of nicotine content plays a very important role in the quality of tobacco products. The level of nicotine content in tobacco leaves directly affects the quality, but the nicotine content of different types of tobacco leaves has a moderate index. The increased nicotine content negatively affects the quality of the tobacco leaf, which is one of the reasons why high-quality tobacco leaves require chemical composition coordination. [0003] Good flue-cured tobacco upper leaves play a leading role in the formulation of modern blended...

AI Technical Summary

Problems solved by technology

At present, the way to reduce nicotine is: 1. Adjust the environmental conditions of tobacco growth and apply effective comprehensive agricultural measures: such as appropriate application of nitrogen and phosphorus fertilizers, increasing the use of potassium fertilizers, using lime and proper use of cake fertilizers, adjusting the pH value, and adjusting the pH value according to the tobacco plant. Regular watering for growth and development, timely transplanting, timely topping according to actual conditions, proper leaf retention, application of growth regulators, appropriate harvesting, optimization of conditioning conditions, and use of aging and fermentation to comprehensively reduce nicotine content. The method has the disadvantages of unstable nicotine reduction effect between years, too many operation links, scattered farmers and difficult operation.

2. In cigarettes, by improving the filtration efficiency and diluting the smoke, these measures can adjust the nicotine in the smoke arbitrarily, but there is often no suitable selectivity, which makes it difficult for consumers to accept

However, this measure is difficult to solve the problems such as the period of use of the strain and the increase in the moisture content of the tobacco leaves after application, which may easily cause mildew in the tobacco leaves, and has not been adopted in the factory.