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Arthrobacter with aerobic denitrification capability and application thereof

A technology of Arthrobacter and nitrite, which is applied in the direction of bacteria, chemical instruments and methods, and methods based on microorganisms. It can solve the problems of excellent strains and their few applications, and achieve strong tolerance, removal ability, and denitrification high efficiency effect

Active Publication Date: 2015-08-26
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many studies on the isolation, screening and mechanism of aerobic denitrifying bacteria at home and abroad, there are few excellent strains and their applications, which still cannot meet the needs of current industrial production.

Method used

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  • Arthrobacter with aerobic denitrification capability and application thereof
  • Arthrobacter with aerobic denitrification capability and application thereof
  • Arthrobacter with aerobic denitrification capability and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030]Example 1 Enrichment, isolation and identification of Arthrobacter sp. DYS3-4

[0031] 1. Enrichment and isolation of bacterial strains: Take 10 mL of sewage samples from Zhangzhou vannamei mariculture pond, add 90 mL of enrichment medium (sodium acetate 1 g / L, sodium citrate 2.5 g / L, NaCl 30 g / L, KH 2 PO 4 2g / L, Na 2 HPO 4 3g / L, MgSO 4 ·7H 2 O 0.05g / L, FeSO 4 ·7H 2 O 0.02g / L, CaCl 2 0.02g / L, distilled water 1000mL, pH 7.6~8.2, sterilized at 121℃ for 20min), with 0.5g / L NO 2 - -N as the only nitrogen source, 15°C, 160rpm / min shaking culture, the first enrichment of aerobic denitrifying bacteria. After 15 days, take 10mL sample liquid from the initial enrichment culture medium, transfer it to fresh enrichment culture medium, and add 1.0g / L NO 2 - -N as the only nitrogen source, 15°C, 160rpm / min shaking culture, for the second enrichment. After 15 days, take 10mL sample solution from the secondary enrichment medium, transfer it to fresh enrichment medium, add 1...

Embodiment 2

[0039] Example 2 Denitrification effect of Arthrobacter sp. DYS3-4 on nitrate and nitrite

[0040] Scrape fresh bacteria from the MA plate, resuspend with sterile saline, and adjust the bacterial concentration to 10 9 cfu / mL, inoculate with 100mg / L NO according to 2% inoculum 3 - -N or NO 2 - -N nitrogen-containing simulated wastewater, cultivated in a shaker at 30°C and 160r / min, and took samples every 4 hours to detect the concentration of nitrite and nitrate in the wastewater, and the concentration of bacteria OD 600 , to determine the denitrification effect of the strain. The composition of simulated wastewater is as follows: sodium succinate 3.5g / L, sodium citrate 2.5g / L, sodium acetate 1g / L, glucose 5g / L, KH 2 PO 4 2g / L, Na 2 HPO 4 3g / L, MgSO 4 ·7H 2 O 0.05g / L, FeSO 4 ·7H 2 O 0.02g / L, CaCl 2 0.02g / L, distilled water 1000mL, sterilized at 121℃ for 20min. Nitrate nitrogen was determined by GB T12763 zinc cadmium reduction method; nitrite nitrogen was determin...

Embodiment 3

[0041] Example 3 Denitrification effect of Arthrobacter sp. DYS3-4 on nitrite-containing wastewater at different temperatures

[0042] Method is with embodiment 2. Arthrobacter sp. DYS3-4 was inoculated in 100mg / L NO 2 - -N is the only nitrogen source in the simulated wastewater, set different temperatures (5°C, 10°C, 15°C, 25°C, 30°C, 35°C, 40°C, 45°C), shake culture at 160r / min, and sample every 12h Determination of bacterial turbidity (OD) in wastewater 600 ) and nitrite concentration. see results Figure 1~5 , the results show that at 15°C, 25°C, 28°C and 35°C, Arthrobacter sp. DYS3-4 grows fast and has a high nitrite removal efficiency, which can be removed in 72, 48, 48 and 60 hours, respectively. 100mg / L NO 2 - -N is completely removed. It can also grow and denitrify normally at 10°C, and can reduce 100mg / L NO within 5 days 2 - -N is completely removed. In addition, at 5°C, for 100 mg / L NO within 10 days 2 - The removal rate of -N reaches 50%.

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Abstract

The invention discloses an arthrobacter with aerobic denitrification capability and application thereof and belongs to the technical field of microorganism application. The arthrobacter DYS3-4 with aerobic denitrification ability is preserved in the China Center for Type Culture Collection, the preservation date is 29th, April, 2015 and the preservation number is CCTCC M 2015 264. The arthrobacter DYS3-4 can be applied to nitrogenous effluent biological denitrification, mariculture nitrite removal, freshwater aquaculture nitrite removal and low temperature denitrification microorganism preparation. Under an aerobiotic condition, the nitrate and the nitrite in the water environment can be rapidly and effectively removed. The arthrobacter growth temperature scope is wide, the denitrification effect at the temperature of 10-35 DEG C is good, and the arthrobacter has good application prospect in the nitrogenous effluent biological denitrification and the freshwater aquaculture nitrite removal.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and in particular relates to an Arthrobacter sp. DYS3-4 with aerobic denitrification ability and its application. Background technique [0002] In natural water bodies, nitrogen exists in nine different valence forms from -3 to +5. Under the joint action of biotic and abiotic factors, they are continuously transferred and transformed in the water body, forming a complex dynamic cycle. Among them, ammonia nitrogen and nitrite can lead to acidification and eutrophication of lakes, rivers, and offshore oceans. At the same time, as potentially toxic inorganic nitrogen compounds, they also have a toxic effect on aquaculture organisms. How to remove nitrogen pollution in water economically and efficiently has become a key research content in the field of water pollution control. [0003] At present, the methods used in denitrification of water body mainly include physical method, che...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/06C02F101/16
CPCC02F3/34C02F2101/16C12N1/205C12R2001/06
Inventor 邵宗泽王丽萍李小义
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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