Uses of icaritin in preparing medicament for preventing and treating endotoxemia

A technology for endotoxemia and icariin, which is used in drug combinations, antidote, blood diseases, etc.

Inactive Publication Date: 2009-05-13
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the use of icariin in the prevention and treatment of endotoxemia

Method used

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  • Uses of icaritin in preparing medicament for preventing and treating endotoxemia
  • Uses of icaritin in preparing medicament for preventing and treating endotoxemia
  • Uses of icaritin in preparing medicament for preventing and treating endotoxemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Effect of Icaritin on the Mortality of Mice After Endotoxin Challenge

[0019] Experimental drugs: icariin (Shanghai Ronghe Medicine, batch number: 20081578, HPLC purity 98%), dexamethasone (sigma, batch number: D4902), LPS (sigma, L2880).

[0020] Experimental animals: C57BL / 6J mice, half male and half male, 18-22g (Shanghai slack), observed in the observation room for 3 days before administration, the animals were randomly divided into groups, with an average of 6 mice per cage, and the room temperature was 20°C to 25°C. ℃, the relative humidity is 50% to 70%, and the light-dark cycle is 12h.

[0021] Experimental method: The experimental animals were randomly divided into 6 groups: blank group, model group, low-dose icariin group, middle-dose icariin group, high-dose icariin group, and dexamethasone control group. 12 only. Among them, 25mg / kg.d, 50mg / kg.d, 100mg / kg.d icariin, 5mg / kg. d Intragastric administration of dexamethasone for 3 consecutive days, ...

Embodiment 2

[0027] Example 2 In vitro experiment of the effect of icariin on endotoxin-related inflammation in mice

[0028] Experimental drug: with embodiment 1.

[0029] Cell line: mouse macrophage cell line RAW264.7 (purchased from Shanghai Cell Bank, Chinese Academy of Sciences).

[0030] experimental method:

[0031] (1) Culture and passage of RAW264.7 cells

[0032] Mouse macrophage RAW246.7 was added to high-glucose DMEM containing 10% fetal bovine serum, 100U / ml penicillin, and streptomycin, and placed in a 5% CO2, 37°C incubator to grow, and the cells grew to 70-80% Subculture after fusion, once every 2-3 days.

[0033] (2) RAW264.7 cell viability detection:

[0034] Adjust the cell suspension concentration to 1×10 5 / ml, add to 96-well culture plate, 100 μl per well, put in 37℃ 5% CO2 incubator and cultivate for 6h, suck out the old culture solution, add icariin (1ug / ml, 10ug / ml, 100ug / ml respectively) ), medium containing dexamethasone (5ug / ml), medium containing 0.1% DMS...

Embodiment 3

[0054] Example 3 In vivo experiment of the effect of icariin on endotoxin-related inflammation in mice

[0055] Experimental drug: with embodiment 1.

[0056] Experimental animal:: with embodiment 1.

[0057] Animal modeling and drug intervention: Experimental animals were randomly divided into 4 groups: blank group, model group, treatment group and western medicine control group, 8 animals in each group; treatment group (icariin 25 mg / kg), western medicine control group (ground Symetasone 5mg / kg) was intragastrically administered with DMSO, and the blank group and the model group were intragastrically administered with DMSO of the same volume; 2 hours after intragastric administration, LPS1mg / kg was injected intraperitoneally, and the blank group was injected with normal saline. Under sodium anesthesia, blood was collected from the heart, and the lungs were separated.

[0058] Detection of TNF-α and IL-6: Let the whole blood stand at room temperature for more than 30 minute...

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Abstract

The invention belongs to the field of traditional Chinese medicine pharmacy, and relates to the novel medical usage of icaritin, in particular to the usage of icaritin in preparation of medicine for preventing and treating endotoxemia. Lipopolysaccharide (LPS) is used for stimulating the macrophage system RAW264.7 of a mouse and establishing an extraneous endotoxic inflammation model, LPS is used for stimulating the C57BL / 6J mouse and establishing the endotoxemia animal model, icaritin is adopted for intervention, and dexamethasone is used as reference. The experiment result shows that the icaritin can reduce the death rate, the level of the inflammatory factor, the inflammatory mediator and the adhesion molecules and infiltration of inflammatory cells of the tissue after the mouse is attacked by endotoxin, and proves that the icaritin can effectively prevent and treat endotoxemia and can be used for further preparing effective drug for preventing and treating endotoxemia, including dosage forms such as oral and enteric capsules or intravenous injections.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and relates to a new medical application of icariin, in particular to the application of icariin in preparing medicines for preventing and treating endotoxemia. Background technique: [0002] Bacterial endotoxin (endotoxin) is the cell wall lipopolysaccharide component (lipopolysaccharide, LPS) released when Gram-negative bacteria grow or cleaved when they die. The prior art discloses that LPS is an important cause of endotoxemia, which can stimulate the body to produce pro-inflammatory cytokines such as TNF-α and IL-6, and the latter triggers a cascade inflammatory cascade reaction, further activating inflammatory cells (monocyte -Macrophages, neutrophils, etc.), increase the expression of adhesion molecules (CD11b / CD18, ICAM-1), release inflammatory mediators (NO, PEG2), form a complex inflammatory network, cause tissue damage and microcirculation disturbance . Studies hav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P39/02A61P7/00
Inventor 董竞成吴金峰
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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