Circulating tumor cell detection chip and application thereof

A tumor cell and chip technology, applied in tumor/cancer cells, animal cells, vertebrate cells, etc., can solve the problems of non-specific adsorption of non-target cells, false positives, high production costs, etc.

Inactive Publication Date: 2016-08-17
WISDOM HEALTHY BIOTECH XIAMEN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main problems in the detection of circulating tumor cells include: 1. The capture efficiency is low, and false negatives are prone to occur
In addition, the use of a large number of antibodies with high production costs and high storage and transportation costs directly leads to an increase in the cost of the final test, and the cost of a test for Cell Search is as high a...

Method used

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  • Circulating tumor cell detection chip and application thereof
  • Circulating tumor cell detection chip and application thereof
  • Circulating tumor cell detection chip and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Circulating tumor cell capture chip and capture device

[0039] see figure 1 and Figure 7 , to make a chip, the chip includes two layers of PDMS and a layer of glass chip, and the PDMS, PDMS, and glass chip are bonded in sequence; the chip is provided with three sample holes and three sample holes; between the sample hole and the sample hole is The triangular micropillar array is arranged according to the principle of deterministic lateral displacement.

[0040] In this embodiment, three sample injection holes are arranged at the left end, and three sample outlet holes are arranged at the right end.

[0041] see Figure 7 C, The air-driven control sampling device for microfluidic chips to capture circulating tumor cells, including: the front and rear two sampling ports on the left side of the chip are respectively connected to a buffer container through pipelines, and the middle sampling port on the left side is connected to the blood sample The contai...

Embodiment 2

[0044] figure 2 Paths through the triangular microarray for particles of different sizes. figure 2 A is the theoretical simulation path of 20 micron and 10 micron particles, the moving path of 20 micron particles is at the bottom, and the moving path of 10 micron particles is at the top;

[0045] The simulation is carried out by COMSOL Multiphysics software.

[0046] figure 2 B is the actual movement path of different particles, the upper path is the path of small particles, and the lower path is the path of large particles.

[0047] At this time, no antibody or aptamer is bound to the triangular column.

Embodiment 3

[0049] The simulation is carried out by COMSOL Multiphysics software. image 3 A is the calculation result of the collision probability of 10, 15, 20 micron particles and triangular microarray; image 3 B is the trend of the collision probability of circulating tumor cells and blood cells on the triangular microarray, where the left, middle and right represent circulating tumor cells, white blood cells and red blood cells, respectively.

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Abstract

The invention relates to a circulating tumor cell detection chip and an application thereof. The above micro-fluidic chip is combined with deterministic lateral displacement and specific bio-affinity identification principles to realize synergistic high-efficiency and high-purity capturing and detection of circulating tumor cells in peripheral blood. A triangular microcolumn array modified with affinity identification molecules, such as a nucleic acid aptamer or antibody, and having a specific geometric arranging shape, is designed in the micro-chip. The rotating angle, the distance, the offset angle and other parameters of triangular arrays are regulated to control the flow behaviors of different dimensions going through micro-channels. The chip can realize three functions in order to improve the capture efficiency and the purity of the circulating tumor cells. A gas-driven sample introduction system is also designed in the invention, and can realize simultaneous control of a plurality of fluid paths through one gas pressure path.

Description

technical field [0001] The invention relates to the design, theoretical simulation and application of a circulating tumor cell detection chip, and relates to the field of microfluidic chips and clinical diagnosis. Background technique [0002] With the in-depth research on the mechanism of tumor metastasis, more and more people realize the importance of early diagnosis, timely treatment and prognosis detection of tumors in reducing cancer mortality, reducing and controlling the incidence of cancer, and more aware of the importance of the circulation The detection of tumor cells plays an important role in early detection of tumor metastasis, monitoring of postoperative recurrence, evaluation of curative effect and prognosis, and selection of appropriate individualized treatment. Therefore, more and more modern detection techniques have been developed and applied to the detection of circulating tumor cells. However, the biggest challenge in the detection of circulating tumor c...

Claims

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Application Information

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IPC IPC(8): C12M1/36C12M1/00C12N5/09C12Q1/02
CPCC12M23/16C12M41/40C12M47/04C12N5/0693G01N33/5005
Inventor 杨朝勇梅塔格斯·加绍·艾哈迈德宋彦龄朱志
Owner WISDOM HEALTHY BIOTECH XIAMEN CO LTD
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