Circulating tumor cell detection chip and application thereof

Abstract

The invention relates to a circulating tumor cell detection chip and an application thereof. The above micro-fluidic chip is combined with deterministic lateral displacement and specific bio-affinity identification principles to realize synergistic high-efficiency and high-purity capturing and detection of circulating tumor cells in peripheral blood. A triangular microcolumn array modified with affinity identification molecules, such as a nucleic acid aptamer or antibody, and having a specific geometric arranging shape, is designed in the micro-chip. The rotating angle, the distance, the offset angle and other parameters of triangular arrays are regulated to control the flow behaviors of different dimensions going through micro-channels. The chip can realize three functions in order to improve the capture efficiency and the purity of the circulating tumor cells. A gas-driven sample introduction system is also designed in the invention, and can realize simultaneous control of a plurality of fluid paths through one gas pressure path.

Description

technical field [0001] The invention relates to the design, theoretical simulation and application of a circulating tumor cell detection chip, and relates to the field of microfluidic chips and clinical diagnosis. Background technique [0002] With the in-depth research on the mechanism of tumor metastasis, more and more people realize the importance of early diagnosis, timely treatment and prognosis detection of tumors in reducing cancer mortality, reducing and controlling the incidence of cancer, and more aware of the importance of the circulation The detection of tumor cells plays an important role in early detection of tumor metastasis, monitoring of postoperative recurrence, evaluation of curative effect and prognosis, and selection of appropriate individualized treatment. Therefore, more and more modern detection techniques have been developed and applied to the detection of circulating tumor cells. However, the biggest challenge in the detection of circulating tumor c...

AI Technical Summary

Problems solved by technology

[0003] At present, the main problems in the detection of circulating tumor cells include: 1. The capture efficiency is low, and false negatives are prone to occur

In addition, the use of a large number of antibodies with high production costs and high storage and transportation costs directly leads to an increase in the cost of the final test, and the cost of a test for Cell Search is as high as $600

2. In the background of high background blood cells, non-specific cell capture is easy to occur, resulting in false positives

3. Large damage to captured cells, low release efficiency, difficult to single-cell analysis of captured circulating tumor cells

However, these methods still have problems such as low capture efficiency, non-specific adsorption of non-target cells, and poor cell activity.

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