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Single-wall carbon nano tube-based ultrasensitive deoxyribonucleic acid (DNA) biosensor and preparation method and application thereof

A technology of single-walled carbon nanotubes and biosensors, applied in the field of bioelectrochemical sensors and their preparation, can solve the problems of increasing costs and limiting the scope of application, and achieve the prevention of interference in signal conduction, better surface area, and strong discrimination of single bases Effects of Mismatched Capabilities

Active Publication Date: 2011-11-30
WENZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Unfortunately, this method needs to introduce a specific substrate, which needs to produce fluorescence or color after the enzyme-catalyzed reaction, which increases its cost and limits its application to a certain extent.

Method used

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  • Single-wall carbon nano tube-based ultrasensitive deoxyribonucleic acid (DNA) biosensor and preparation method and application thereof
  • Single-wall carbon nano tube-based ultrasensitive deoxyribonucleic acid (DNA) biosensor and preparation method and application thereof
  • Single-wall carbon nano tube-based ultrasensitive deoxyribonucleic acid (DNA) biosensor and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) Preparation of SWCNTs-Au electrodes

[0047] Such as figure 1 As shown, the chemical vapor deposition (CVD) method is used. First, the temperature of the quartz tube is rapidly raised to 1000 °C in a heating furnace, and then a mixed gas of ethanol / helium is introduced, and Fe / Mo nanoparticles are used as catalysts. The high-temperature reduction reaction is carried out for 15 minutes. Single-walled carbon nanotube arrays were obtained by in situ growth on a silicon substrate. Then connect the electrode with a copper wire as a working electrode, 10ml (1mM) of HAuCl 4 As a gold source, use Pt wire as the counter electrode, use the method of constant potential, and adjust the voltage to -0.5V. After depositing with the two-electrode system for 10s, turn off the power, rinse with MilliQ water, and dry the electrode surface with ear washing balls. Get SWCNTs-Au electrode.

[0048] (2) Preparation and hybridization of sensing electrodes

[0049] The DNA sequence sel...

Embodiment 2

[0056] Obtain the sensing electrode according to the method in Example 1, put it into the matching DNA solution with a concentration of 0.1 μM, hybridize at 45°C for 10 h to form SWCNTs-Au-dsDNA, take it out and rinse the electrode surface repeatedly with MilliQ water and Tris-HCl buffer solution, Wash away unhybridized DNA non-specifically adsorbed on the electrode surface. The electrochemical impedance curve was recorded according to the method in Example 1, and the results were consistent with Example 1.

Embodiment 3

[0058] Obtain the sensing electrode according to the method in Example 1, put it into the matching DNA solution with a concentration of 1nM, hybridize at 45°C for 2h to form SWCNTs-Au-dsDNA, take it out and wash the electrode surface repeatedly with MilliQ water and Tris-HCl buffer solution , to wash away unhybridized DNA non-specifically adsorbed on the electrode surface. Record the electrochemical impedance curve by the method in embodiment 1 (as figure 2 Curve l in A).

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Abstract

The invention provides a single-wall carbon nano tube-based ultrasensitive deoxyribonucleic acid (DNA) biosensor and a preparation method and application thereof. In the method, single-wall carbon nano tubes (SWCNTs) are grown on the surface of a silicon wafer on site by a chemical vapor deposition method, and gold nano particles are deposited on the surfaces of carbon nano tube electrodes by an electrochemical deposition technology. A single-stranded DNA (ssDNA) probe is self-assembled to the surfaces of SWCNTs-Au electrodes and subjected to hybridization reaction with complementary ssDNA. The change of electron transfer resistance before and after hybridization is recorded by utilizing the unique specific surface areas of the carbon nano tubes and the quick dynamic characteristics of the electrodes and by an electrochemical impedance method under the action of current signal amplification of nanometer gold to realize the quantitative detection of the complementary DNA. The detection limit of the sensor on the target DNA can reach between 10 and 20 M, so the sensor has the advantages of high sensitivity and selectivity, capability of being used repeatedly and the like, and has important significance in fields of medical diagnosis, the food industry, environment friendliness and the like.

Description

technical field [0001] The invention relates to the field of bioelectrochemical sensors and their preparation, in particular to a deoxyribonucleic acid (DNA) electrochemical sensor and its preparation method and detection application. Background technique [0002] Genetic diagnosis is a method of diagnosing diseases by directly detecting the presence or defect of genes. The detection object of genetic diagnosis is deoxyribonucleic acid or ribonucleic acid (RNA). In terms of the methodology of genetic diagnosis, restriction endonuclease spectrum analysis, nucleic acid molecular hybridization, restriction fragment length polymorphism linkage analysis, polymerase chain reaction (PCR), and DNA sensors developed in recent years have been established successively. and DNA chip technology. Labeled nucleic acid hybridization detection technology has been widely used in biology, medicine, environmental science and other related fields, but the inspection process is time-consuming a...

Claims

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Application Information

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IPC IPC(8): G01N27/416G01N27/327
Inventor 王舜陈锡安李玲金辉乐
Owner WENZHOU UNIVERSITY
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