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Method for controlling postharvest diseases of grapes and preservative

A preservative and grape technology, applied in pest control, fruit and vegetable preservation, botanical equipment and methods, etc., can solve problems such as threats to food safety, poor drug resistance, large pollution, etc., and achieve simple preparation process and long action period , the effect of reducing the incidence of

Inactive Publication Date: 2020-07-17
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the action mechanism of fungicides is relatively simple, and the propagation speed of pathogenic bacteria is fast, so the resistance is also fast, and the pollution is large and the residue is high, threatening human food safety.
Calcium propionate, as a new type, green, environmentally friendly organic salt, has less pollution, is degradable, and has the advantages of low toxicity, poor drug resistance, and high safety. It is usually used in food and medicine and other fields. At the same time, it has a significant effect on common pathogenic bacteria such as Aspergillus flavus and Fusarium, etc. on food and grain, but there is no report on the control method of applying calcium propionate to the field to control grape postharvest diseases and prolong the storage and preservation period.

Method used

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  • Method for controlling postharvest diseases of grapes and preservative
  • Method for controlling postharvest diseases of grapes and preservative
  • Method for controlling postharvest diseases of grapes and preservative

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: the influence of different concentrations and different pH value calcium propionate treatment on Botrytis cinerea mycelium growth

[0039] 1. Materials and methods

[0040] 1. Calcium propionate

[0041] Calcium propionate is a finished product purchased by Shanghai Macklin Biochemical Technology Co., Ltd., with a purity of ≥99.5% (analytical grade, AR).

[0042] Preparation of 10% mother liquor of calcium propionate at various pH values:

[0043] pH=5 calcium propionate mother liquor: add 12g calcium propionate to 113ml of distilled water, add 7ml of 1mol / L HCl solution to make 10% mother liquor, calibrate with pH test paper, filter and sterilize and store at 4°C for later use.

[0044] pH=6 calcium propionate mother liquor: Weigh 10g of calcium propionate into 100ml of distilled water, add 2-4ml of 1mol / l Hcl solution to adjust the pH value, use pH test paper to calibrate, filter and sterilize and store at 4°C for later use.

[0045] Conventional calc...

Embodiment 2

[0067] Example 2: Inhibitory effect of low pH value novel antiseptic preservative treatment on various plant pathogenic bacteria

[0068] 1. Materials and methods

[0069] Botrytis cinerea, Alternaria alternata, Colletotrichum gloeosporioides, and Fusarium graminearum are common pathogenic fungi isolated from grape fruits in the laboratory of our research group .

[0070] Use the 10% (w / v) calcium propionate mother liquor (pH value 5) prepared in Example 1 and the culture medium to be formulated into calcium propionate mass percent and be respectively 0,0.1%, 0.2%, 0.4%, pH value Both are medium of 5.

[0071] Others, such as the medium PDA used, the preparation method, the strain activation method, and the determination of the inhibitory effect of the new antiseptic preservative, etc., are as described in the "Materials and Methods" section of Example 1.

[0072] 2. Measurement results

[0073] The result is as Figure 2a , Figure 2b , Figure 2c , Figure 2d As show...

Embodiment 3

[0074] Embodiment 3: Containing the effect of calcium propionate novel antiseptic preservative to control botrytis cinerea disease

[0075] 1. Materials and methods

[0076] Fruit: Sunshine rose grape (Vitis vinifera L. Muscat-shine) was used as the experimental material. The fruit comes from Shanghai Malu Grape Research Institute. The fruits were immediately transported to the School of Life Sciences of East China Normal University after harvesting.

[0077]Pathogen: Botrytis cinerea (B.cinerea) spore suspension concentration is 1x10 5 (spores / ml).

[0078] Treatment method: Each grape fruit is inoculated with 20ul of the following treatment solution after being stabbed: A, a new antiseptic preservative containing 0.5% calcium propionate by mass percentage; B, a new antiseptic preservative containing 1% calcium propionate by mass percentage C, a novel antiseptic preservative containing 1.5% calcium propionate by mass percentage; D, distilled water (CK) (the inoculum size ...

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Abstract

The invention discloses a method for controlling postharvest storage diseases before picking. The method comprises the following steps: after grape blossom, respectively spraying calcium propionate solution with a final concentration of 0.5-1.5% (w / v) in a young fruit period, in a middle fruit period, in a mature period and before picking; and after picking, grapes are put into a refrigerator or acold storage, wherein the preservation period is prolonged by 30 days or above compared with the preservation period of grape fruits which are not treated. The method disclosed by the invention has an obvious inhibition effect on common storage diseases of grapes; meanwhile, according to laboratory bacteriostatic experiments and field practical applications, the fruit cracking rate in the early stage of the grapes can be obviously reduced, the incidence rate and the falling rate of grapes in the field and in a post-harvest storage process can be inhibited, and the fruit quality can be well maintained. The method disclosed by the invention has the advantages of simple flow, no toxicity, no pollution and low cost and has a huge practical application value.

Description

technical field [0001] The invention belongs to the field of postharvest disease control of fruits and vegetables, and in particular relates to an antiseptic calcium propionate, formulation preparation, a preharvest application method and a method for effectively controlling grape postharvest disease during postharvest storage. Background technique [0002] Grapes are highly susceptible to microbial infection due to their high water content and sugar content, and their thin skins are easily damaged. Pathogenic fungi mostly intrude latently from the field, which can cause grape disease during post-harvest storage, causing grape rot and shattering. Botrytis cinerea, Alternaria, Colletotrichum, Aspergillus and other fungi are common pathogens that cause postharvest diseases of grapes. Among them, Botrytis cinerea will not only cause grape yield reduction during the flowering period, At the same time, its strong low-temperature growth ability and drug resistance make it the dom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/154A23B7/157A23L19/00A01G13/00A01N37/02A01P3/00A01P21/00
CPCA23B7/154A23B7/157A23L19/00A01G13/00A01N37/02
Inventor 许玲孙晨旭朱品宽薛永军
Owner EAST CHINA NORMAL UNIV
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