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Bacillus amyloliquefaciens strain as well as preparation method and application of strain powder preparation of bacillus amyloliquefaciens strain

A technology of Bacillus amyloliquefaciens and Bacillus, which is applied in the field of microbiology, can solve the problems that Bacillus amyloliquefaciens has a single antagonism, common aquatic pathogens do not have broad-spectrum antagonism, and achieve the effect of increasing cross-infection of pathogens

Active Publication Date: 2016-01-13
江苏省苏微微生物研究有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The antagonistic effect of Bacillus amyloliquefaciens in this patent is single and does not have broad-spectrum antagonistic effect on common aquatic pathogens
There is no patent report on this aspect of Bacillus amyloliquefaciens

Method used

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  • Bacillus amyloliquefaciens strain as well as preparation method and application of strain powder preparation of bacillus amyloliquefaciens strain
  • Bacillus amyloliquefaciens strain as well as preparation method and application of strain powder preparation of bacillus amyloliquefaciens strain
  • Bacillus amyloliquefaciens strain as well as preparation method and application of strain powder preparation of bacillus amyloliquefaciens strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Screening of antagonist bacteria

[0023] The sample is collected in Wuxi Goose Lake Cream Pond, which is called 10g of mud at the bottom of the pond in a 250ml triangle bottle equipped with 90ml of sterile water and a small amount of glass beads.Each sample is absorbed 1.0ml, heated at 80 ° C for 10min, and removed 0.1ml on the nutritional agar, coated, and cultivated 37 ° C.Selected a well -growing colonies separated and purified 3 times to obtain a single colonies.

[0024] Take the instructions (water-loving and gas alone, mild gas monocyloburate) 0.1ml evenly coated on the LB tablet, plant this tablet with the above single bacteria, and put it in a constant temperature culture box of 25-30 ° C.The strains with the display number JSSW-LA have antagonistic effects on hydrophone and mild gas monocyloburate, producing significant transparent bacteriostatic rings.

Embodiment 2

[0025] Example 2: Bacterial identification

[0026](1) Morphological characteristics: The strain JSSW-LA, Gram-staining is positive, the mirror test is rod-shaped, and the cell diameter is 1-2 μm, which can form spores.A large amount of circular, smooth, opaque, and white colonies can be obtained at the LB medium.

[0027] (2) Biochemical characteristics: strain JSSW-LA, Gram-dyeing is positive, contact enzymes, oxidase, VP tests, positive, can hydrolyze starch, brilliant glue, tentinopus, can grow in the environment of 50 ° C.β-galactogenase is negative.

[0028] The results of the growth experiment show that the strain can use ɑ D-D-glucose, D-fructose, d-sorgeol, D-mananol, glycerin, lactose, D-seaweed sugar, dragon diet, sucrose, pectin, pectin, pectin,, L-lactic acid, citric acid, L-apple acid, bromotinic acid, L-alanine, L-冬 ,, L-glutamic acid, n-acetyl-β-D-glucosamine reactionFor positive.

[0029] The results of chemical sensitivity experiments show that the strain is pyri...

Embodiment 3

[0032] Example 3: bacteriostatic measurement

[0033] Sterile filter paper at a concentration of 3.0 × 10 7 CFU / ML Dexybacteria JSSW-LA fresh culture solution is soaked in 1.0H.The concentration is about 5.0 × 10 6 CFU / ML's aquaculture of aquaculture in aquaculture of pathogenic bacteria, mild gas monocyloburate, guinea pig gas monocycus, Edward bacteria, Vitamin monocycus, eelson bacteria, paramail hemolytic dissolving hemolysisThe liquid culture liquid of sexococcini, algae, and E. coli is 0.1ml, coated on the LB agar culture dish, and then paste the filter paper soaked in the liquid on the dine, 3 tablets for each flat dish,Each flat dish is made 3 repetitions.The flat dish is placed in the 28 ° C culture box 24h and 48h to measure the size of the bacteriostatic ring.

[0034] The measurement results are shown in Table 1:

[0035] Table 1 Decadee Bacteria JSSW-LA bacteriostatic measurement results (diameter of bacteriostatic circle: MM)

[0036]

[0037] Note: "-" means no ba...

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Abstract

The invention discloses a bacillus amyloliquefaciens strain as well as a preparation method and application of a strain powder preparation of the bacillus amyloliquefaciens strain, and belongs to the technical field of microorganisms. The bacillus amyloliquefaciens JSSW-LA, which is obtained through screening, is preserved in China Center for Type Culture Collection with preservation number of CCTCC NO: M2015602. The strain powder preparation of the bacillus amyloliquefaciens, which is prepared through freeze-dried culture activation, fermentation culture and strain powder preparation, is applicable to an additive of fishery breeding feed or a water quality improver of aquaculture; the bacillus amyloliquefaciens JSSW-LA provided by the invention is capable of effectively inhibiting the growth of A.sobria, A.hydrophila, A.caviae, aeromonas veronii and edwardsiella tarda, and an inhibitory effect on the A.sobria, A.hydrophila and A.caviae is more excellent than that on other pathogenic bacteria. Therefore, the JSSW-LA strain powder, applied to aquaculture, is capable of effectively preventing and controlling diseases in aquaculture animals caused by pathogenic bacteria, in particular bacterial bleeding disease, so as to protect the healthy growth of aquatic animals.

Description

Technical field [0001] The present invention involves the preparation method and application of a strain strain strain and its bacterial powder preparation, which belongs to the field of microbial technology. Background technique [0002] In recent years, my country's aquaculture industry has flourished. However, with the increasing scale of high -density intensive breeding, the ecological environment of the aquaculture has deteriorated, which has greatly increased the opportunities for cross -lesion infection between aquatic animals.Freshwater fish bacterial hemorrhage is the most serious acute infectious disease in the history of fish farming in my country. It often leads to a large number of deaths of farming and wild fish, causing serious losses in aquaculture.Water -only qi monocyclic bacteria ( A.hydrophila ), Mild gas single bacteria ( A.Sobria ), Guinea pig gas single bacteria ( A.caviae ) It is the main pathogen of bacterial hemorrhage. They exist in water, soil, and aqu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/80C02F3/34C12R1/07C02F103/20
CPCY02A40/818
Inventor 孙梅张维娜陈秋红高亮施大林匡群张宪中邹益东夏冬
Owner 江苏省苏微微生物研究有限公司
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