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Bacillus velezensis and its application in plants

A technology of bacillus and plants, applied in the direction of application, plant growth regulator, plant growth regulator, etc., can solve the problems of insufficient depth and little research, and achieve the effect of reducing use, promoting plant growth, and protecting quality and safety

Active Publication Date: 2018-04-27
卢志军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The current research and development on Bacillus is mainly concentrated on a few species such as Bacillus thuringiensis and Bacillus subtilis. As a new type of biocontrol bacteria with great potential, Bacillus velei has few related studies and is not deep enough. Industrialization development and its application in plant disease prevention and growth promotion need to be further strengthened

Method used

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  • Bacillus velezensis and its application in plants
  • Bacillus velezensis and its application in plants
  • Bacillus velezensis and its application in plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Isolation, purification and identification of Bacillus velezensis NSZ-YBGJ0001 CGMCC No.14384

[0039] 1. Isolation and purification of Bacillus velezensis NSZ-YBGJ0001 CGMCC No.14384

[0040] Collect 0.1g of soil from the cowpea field in Lingshui County, Sanya City, Hainan Province, add 0.9mL sterile water, and shake it well, which is 10 -1 Diluent, diluted 10 in sequence -3 , 10 -4 , 10 -5 times. Take 0.1 mL of the above suspensions and evenly spread them on beef extract peptone (NA) medium, repeat each concentration 3 times, and place them in a constant temperature incubator at 28°C for 24 hours. After a single colony grows, pick a single bacterial colony and streak it on an NA plate to obtain a purified bacterial strain. Fusarium oxysporum, Fusarium solani, Verticilliumdahliae, Rhizoctonia solani, Sclerotinia sclerotiorum, anthracnose (Colletotrichum spp.), Pyricularia grisea, Alternaria tenuissima, Monilinia fructicola, Colletotrichumgloeosporioides...

Embodiment 2

[0058] Example 2 Activity of Bacillus velezensis NSZ-YBGJ0001 CGMCC No.14384 on plant pathogenic fungi

[0059] The inhibitory activity of Bacillus velezensis NSZ-YBGJ0001CGMCC No.14384 on plant pathogenic fungi was detected by plate culture method.

[0060] Activate Bacillus velezensis NSZ-YBGJ0001 CGMCC No.14384 on the NA plate for 24 hours, pick a single colony and inoculate it into the NB liquid medium, culture it on a shaker at 28°C and 180 rpm for 16 hours. Nephelometric method to determine the concentration of bacterial suspension is 10 8 cfu / ml spare.

[0061] Cultivate the target fungus for testing on a PDA plate at 25°C for 3d-5d, and set aside.

[0062] The spare Bacillus velezensis NSZ-YBGJ0001 CGMCCNo.14384 bacterial liquid was centrifuged, and the supernatant was collected. The supernatant was evenly mixed into the PDA medium below 50°C at a ratio of 1:25, and poured onto a plate for later use.

[0063] Take the spare target fungus plate, and use a puncher (5...

Embodiment 3

[0071] Example 3 Activity of Bacillus velezensis NSZ-YBGJ0001 CGMCC No.14384 on Meloidogyne incognita

[0072]Bacillus velezensis (Bacillus velezensis) NSZ-YBGJ0001 CGMCC No.14384 was activated on the NA plate for 24 hours, picked a single colony and inoculated it into the NA liquid medium, and cultured it on a shaker at 28°C and 180rpm for 16 hours, and then used a microphone to measure the turbidity. The concentration of bacterial suspension was determined by the method of 10 8 cfu / mL spare.

[0073] In a 24-well plate, add 1 mL of nematode suspension (concentration of about 300 heads / mL) to each well, add the above-mentioned spare NSZ-YBGJ0001 bacterial suspension to it quantitatively and dilute it, and configure it as 10, 100, 1000, 5000, 10000 times (NSZ-YBGJ0001 bacterial solution dilution multiple), at this time the concentration of NSZ-YBGJ0001 in each treatment was 10 7 cfu / mL, 10 6 cfu / mL, 10 5 cfu / mL, 0.5×10 5 cfu / mL, 10 8 cfu / mL, 10 4 cfu / mL.

[0074] With ...

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Abstract

The invention discloses Bacillus velezensis and its application in plants and provides a strain of Bacillus velezensis with broad-spectrum disease-preventing and life-promoting activity in a plant anda microbial agent containing the strain as an active ingredient. The Bacillus velezensis is Bacillus velezensis NSZ-YBGJ0001 and has a preservation number of CGMCC No. 14384. The Bacillus velezensisNSZ-YBGJ0001 with the preservation number of CGMCC No. 14384 can effectively prevent and control plant diseases, does not harm to the plants and can promote plant growth to a certain extent. The Bacillus velezensis microbial agent can replace or reduce chemical pesticides, protect the ecological environment to a large extent and keep the quality and safety of agricultural products.

Description

technical field [0001] The invention relates to a strain of bacillus velei and its application on plants, and belongs to the field of microbial bacterial agents for plant disease prevention and growth promotion. Background technique [0002] In recent years, my country's agricultural planting structure has undergone great adjustments, and this adjustment trend will continue for a period of time in the future. The varieties of crops with high added value such as economic crops and horticultural crops are constantly enriched, and the planting area and area are constantly expanding; At the same time, the development mode of my country's planting industry has also undergone tremendous changes, with large-scale planting and protected land production area and proportion rising rapidly; Operation and other factors have brought great challenges to the prevention and control of the occurrence, spread and prevalence of plant diseases. Some key diseases have continued to break out, and s...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P5/00A01P21/00C12R1/07A01N63/22
CPCA01N63/00C12N1/20C12R2001/07C12N1/205A01N63/22A01N25/00A01N25/04C12N1/02
Inventor 卢志军石旺鹏李鹏张小利潘慧英陶永梅付琼
Owner 卢志军
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