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Biological control agent, bioorganic fertilizer, and applications thereof

A technology of biological control agent and bio-organic fertilizer, which is applied in the field of pest prevention, can solve the problems of decreased inhibition of Fusarium oxysporum, weakened control of plant wilt, and increased incidence of plant wilt, so as to reduce diseases Occurrence, improvement of biological control efficiency, improvement of soil physical, chemical and biological properties

Active Publication Date: 2018-11-06
INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after further research, it was found that the Bacillus Velez was affected by the indigenous microorganisms and could not colonize and survive in the soil for a long time, and the prevention and control of plant wilt gradually weakened. In 3 years, its inhibitory effect on Fusarium oxysporum decreased, and the incidence of plant wilt increased

Method used

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  • Biological control agent, bioorganic fertilizer, and applications thereof
  • Biological control agent, bioorganic fertilizer, and applications thereof
  • Biological control agent, bioorganic fertilizer, and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 biological control agent

[0047] Prepare biological control agent according to the following methods, specifically comprising:

[0048] Pick single colonies of H-1, H-6 and H-7 and inoculate them in fresh LB test tube medium; pick H-5 single colony and inoculate them in TSA test tube medium. The antagonistic bacteria seed solution was obtained after culturing on a shaking table overnight, and the culturing conditions were 30° C., 170 r / min.

[0049] The above-mentioned seed solution was transferred to an Erlenmeyer flask equipped with fresh LB or TSA medium according to the ratio of 2%, and cultured on a shaker for 3 days, the culture condition was 30°C, 170r / min; the concentration of antagonistic bacteria in the Erlenmeyer flask was detected Quantity, up to 1.0×10 9 After cfu / mL, centrifuge the bacterial solution under the following conditions: 10000r / min for 5min, discard the supernatant, and collect the bacterial cells.

[0050] The bacterium is resu...

Embodiment 2

[0051] Embodiment 2 biological organic fertilizer

[0052] Prepare bio-organic fertilizer according to the following methods, specifically including:

[0053] Add 5Kg peanut bran and 5Kg rapeseed meal to 50Kg cow dung compost and mix evenly, insert 12L of the biological control agent described in Example 1, adjust the water content of the mixed heap to 50wt%~60wt%, antagonistic bacteria in the heap The initial concentration in is about 10 8 cfu / g dry sample. Stir fully and evenly to keep the nutrient and moisture content of the heap uniform. The heap is placed in a composting reactor to start fermentation, and the heap is turned every 2 days; the heap is ventilated for 6 minutes every 1 hour, and the ventilation rate is 30L / min The heap is maintained at a high temperature of 35-45°C for about 5 days, and after cooling down to below 35°C, the moisture content of the heap is dried to 30wt%-40wt% to obtain bio-organic fertilizer.

Embodiment 3

[0055] Inorganic soil improver is prepared according to the following method, which specifically includes: 300 kg of 100 mesh phosphate rock powder, 670 kg of 100 mesh slaked lime, 22 kg of 100 mesh magnesium sulfate, 5 kg of 100 mesh zinc sulfate, and 3 kg of 100 mesh borax are placed in a mixer, stirred evenly, and obtained Acidic soil conditioner.

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PUM

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Abstract

The invention relates to a biological control agent, a bioorganic fertilizer, and applications thereof. The biological control agent comprises Bacillus velezensis, Bacillus subtilis, Paenibacillus polymyxa, and Bacillus amyloliquefaciens. The provided biological control agent and bioorganic fertilizer can continuously inhibit fusarium oxysporum for a long term, and has a long-lasting control effect on plant blight, in particular, banana blight.

Description

technical field [0001] The invention relates to the field of pest control, in particular to a biological control agent, a biological organic fertilizer and applications thereof. Background technique [0002] Fusarium oxysporum (Fusarium oxysporum) is a worldwide distribution of soil-borne pathogenic fungi with a wide range of hosts, which can cause more than 100 plant wilts in melons, Solanaceae, bananas, cotton, legumes and flowers. Among them, banana fusarium wilt leads to necrosis of vascular bundles of bananas, wilting of stems and leaves and even all withering, which seriously affects the development of banana industry. [0003] At present, the prevention and control methods for plant Fusarium wilt, especially banana Fusarium wilt, mainly include: chemical control, breeding of disease-resistant varieties, agricultural control measures and biological control, etc. The first three measures have pollution to the environment, high cost and prevention However, biological co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F15/00C05F11/08C05G3/04A01N63/00C09K17/06C09K17/14A01P3/00C12R1/07C12R1/125C12R1/12C09K101/00C09K109/00C05G3/80
CPCA01N63/00C05B17/00C05F3/00C05F11/08C09K17/06C09K17/14C09K2101/00C09K2109/00C12N1/20C05G3/80C05F17/00C05D3/02C05D5/00C05D9/02C05F9/04
Inventor 黄建凤逄玉万黄巧义张木唐拴虎付弘婷李苹
Owner INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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