Preparation method of fertilizer containing microorganisms

A technology of microorganisms and microbial agents, applied in the preparation of organic fertilizers, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of inability to exert the effect of microbial agents, the death of "indigenous" microorganisms, and the inability to form population effects, etc. problems, to achieve the effect of improving crop quality, strong survival competitiveness, and strong acid resistance

Inactive Publication Date: 2016-11-09
姚继胜
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Due to the serious soil compaction and acidification, the external temperature is erratic, and the soil environment has its own "indigenous" microorganisms, the microbial agents cultivated in the greenhouse are directly put into the soil, because they cannot adapt to the harsh environment and the competition of "indigenous" microorganisms in the soil. Basically all of them died, even if a small number survived because they could not form a population effect, they would not be able to exert the effect of microbial agents

Method used

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  • Preparation method of fertilizer containing microorganisms

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Experimental program
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Effect test

Embodiment 1

[0036] Add kelp, soybean meal, wheat bran, slaked lime and distilled water into a fermenter at a mass ratio of 200:500:300:1:350 and sterilize at 120°C for 20 minutes to obtain product A. And prepare the fermented seed liquid: inoculate the Lactobacillus acidophilus into the liquid medium at 30° C., and cultivate for 26 hours. The liquid medium is: beef extract 3g, peptone 4g, soluble starch 8g, potassium nitrate 0.8g, potassium dihydrogen phosphate 0.3g, magnesium sulfate 0.6g, ferrous sulfate 0.01g, sodium chloride 1g, agar 15g, distilled water 1000ml, pH6.8, the ratio of Lactobacillus acidophilus and liquid medium is 8:100. Add the seed liquid to product A, wherein the mass ratio of seed liquid to product A is 8:100, and anaerobically ferment for 8 days at 30°C and pH 6.5 to 7.5 to obtain product B; adjust the pH of product B to 4.5-5, cool the temperature of the fermenter to 4°C, store for 4 days, return the temperature to 30°C to obtain product C; add the seed liquid to ...

Embodiment 2

[0038] Add kelp, soybean meal, wheat bran, slaked lime and distilled water into a fermenter at a mass ratio of 200:500:300:2:250 and sterilize at 120°C for 25 minutes to obtain product A. And prepare the fermented seed liquid: inoculate the Lactobacillus acidophilus into the liquid medium at 25° C., and cultivate for 30 hours. The liquid medium is: beef extract 3g, peptone 4g, soluble starch 8g, potassium nitrate 0.8g, potassium dihydrogen phosphate 0.3g, magnesium sulfate 0.6g, ferrous sulfate 0.01g, sodium chloride 1g, agar 15g, distilled water 1000ml, pH6.8; the ratio of Lactobacillus acidophilus to liquid medium is 8:100. Add the seed liquid to product A, wherein the mass ratio of seed liquid to product A is 8:100, and anaerobically ferment for 6 days at 25°C and pH 6.0 to 7.0 to obtain product B; adjust the pH of product B to 4.5~5, cool the temperature of the fermenter to 2°C, store for 3 days, return the temperature to 25°C to obtain product C; add the seed liquid to t...

Embodiment 3

[0040]Add kelp, soybean meal, wheat bran, citric acid and distilled water in a mass ratio of 200:500:300:1:500 into a fermenter and sterilize at 110°C for 30 minutes to obtain product A. And prepare the fermented seed liquid: inoculate the Lactobacillus acidophilus into the liquid medium at 35° C., and cultivate for 24 hours. The liquid medium is: beef extract 3g, peptone 4g, soluble starch 8g, potassium nitrate 0.8g, potassium dihydrogen phosphate 0.3g, magnesium sulfate 0.6g, ferrous sulfate 0.01g, sodium chloride 1g, agar 15g, distilled water 1000ml, pH6.8; the ratio of Lactobacillus acidophilus to liquid medium is 8:100. Add the seed liquid to product A, wherein the mass ratio of seed liquid to product A is 8:100, and anaerobically ferment for 9 days at 35°C and pH5.5-6.5 to obtain product B; adjust the pH of product B to 4.5-5, cool the temperature of the fermenter to 8°C, store for 5 days, return the temperature to 35°C to obtain product C; add the seed liquid to produc...

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Abstract

The invention relates to a preparation method of a fertilizer containing microorganisms. The preparation method comprises: adding dried plant residues, an acid-alkali adjusting agent, distilled water, a culture medium and microorganisms into a fermentation tank, fermenting at a certain temperature, carrying out pH value adjustment and cooling on the product after fermenting for a certain time, storing for 3-5 days in the fermentation tank, adding slaked lime, adjusting the pH value, continuously adding the culture medium and the beneficial microorganism to the fermentation tank, fermenting at a certain temperature, carrying out pH value adjustment and cooling on the product after the fermenting, storing for 3-5 days in the fermentation tank, repeating the steps one time, carrying out air drying on the product, and adding to a fertilizer to obtain the fertilizer containing the microorganisms. According to the present invention, the natural ecological environment of the soil is simulated, and the three fermentations are performed, such that the fermented microbial agent can quickly adapt to the environment after being put into the soil so as to ensure the survival rate.

Description

technical field [0001] The invention relates to a preparation method of fertilizer containing microorganisms. Background technique [0002] The evaluation indicators for agricultural microorganisms mainly include the purity of the microbial agent and the quantity of the microbial agent per unit mass. Therefore, the preparation method of bacterial agents as the prior art is generally: a fermentation process is carried out under the temperature and pH conditions suitable for microorganisms to survive, which is equivalent to greenhouse cultivation. [0003] Due to the serious soil compaction and acidification, the external temperature is erratic, and the soil environment has its own "indigenous" microorganisms, the microbial agents cultivated in the greenhouse are directly put into the soil, because they cannot adapt to the harsh environment and the competition of "indigenous" microorganisms in the soil. Basically all die, even if there is a small amount of surviving because t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/02C05G3/04C05F17/00C12N1/20C12R1/23C05G3/60C05G3/80
CPCC05F5/002C05F17/50C05G3/60C05G3/80C12N1/20C05F11/00C05F11/08Y02W30/40
Inventor 姚继胜
Owner 姚继胜
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