Oocystis concentrate with high activity and high stress resistance, and preparation method and application thereof

A technology of oocystis and stress resistance is applied in the field of oocystis concentrate and its preparation, which can solve the problems of unfavorable shrimp growth, unsuitable for seed expansion, easy pollution, etc. Effects of aging and algal bloom, highly active stress resistance

Pending Publication Date: 2018-08-03
GUANGDONG JINYANG BIOTECHNOLOGY CO LTD
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional way of cultivating oocysts is to cultivate in open cement ponds, which has a large scale of cultivation and high yield, but the disadvantages are: (1) It is easy to pollute, occupies a large area, is greatly affected by the weather, is not easy to control, and the growth of algae cells is slow , the doubling time is long, the one-time investment is large and the output is low, and it is not suitable for the expansion stage; (2) the algal phase of the oocysts cultivated by the traditional method is not stable enough, and it is easy to age and lose algae. It is

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Oocystis concentrate with high activity and high stress resistance, and preparation method and application thereof
  • Oocystis concentrate with high activity and high stress resistance, and preparation method and application thereof
  • Oocystis concentrate with high activity and high stress resistance, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] A method for preparing a highly active and stress-resistant oocyst concentrate, comprising the following steps:

[0056] Step 1. Isolation of pure oocysts

[0057] Oocyst samples were collected from high-yield prawn culture ponds, and then the oocyst cells were separated and selected under a microscope.

[0058] Step 2, the cultivation of pure species oocysts

[0059] (1) Prepare the special culture medium for oocysts according to the following ratio:

[0060]

[0061] The specific gravity of the prepared special medium for oocysts is 1.011, and the pH value is 8.1.

[0062] (2) Preparation of oocyst pure seed liquid

[0063] The oocyst cells selected in step 1 were inoculated into the above-mentioned special medium for oocysts, and were continuously aerated, cultured and purified under the conditions of a temperature of 25°C and a light of 2000 Lx. After 12 to 18 hours, the concentration of oocysts reached 65-75 When 10,000 / ml, stop aerating, drain the supernata...

Embodiment 2

[0090] A method for preparing a highly active and stress-resistant oocyst concentrate, comprising the following steps:

[0091] Step 1, the separation of pure oocysts

[0092] Collect oocyst samples from high-yield prawn culture ponds, and then separate and select oocyst cells under a microscope;

[0093] Step 2, the cultivation of pure species oocysts

[0094] (1) Prepare the special culture medium for oocysts according to the following ratio:

[0095]

[0096] The specific gravity of the prepared special medium for oocysts is 1.014, and the pH value is 8.0.

[0097] (2) Preparation of oocyst pure seed liquid

[0098] The oocyst cells selected in step 1 were inoculated into the above-mentioned special medium for oocysts, and were continuously aerated, cultured and purified under the conditions of a temperature of 23°C and a light of 1800 Lx. After 12 to 18 days, the concentration of oocysts reached 65-75 When 10,000 / ml, stop aerating, drain the supernatant after sedime...

Embodiment 3

[0125] A method for preparing a highly active and stress-resistant oocyst concentrate, comprising the following steps:

[0126] Step 1, the separation of pure oocysts

[0127] Collect oocyst samples from high-yield prawn culture ponds, and then separate and select oocyst cells under a microscope;

[0128] Step 2, the cultivation of pure species oocysts

[0129] (1) Prepare the special culture medium for oocysts according to the following ratio:

[0130]

[0131] The specific gravity of the prepared special medium for oocysts is 1.013, and the pH value is 8.2.

[0132] (2) Preparation of oocyst pure seed liquid

[0133] The oocyst cells selected in step 1 were inoculated into the above-mentioned special medium for oocysts, and were continuously aerated, cultured and purified under the conditions of a temperature of 28°C and a light of 1900 Lx. After 12 to 18 days, the concentration of oocysts reached 65-75 When 10,000 / ml, stop aerating, drain the supernatant after sedime...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention specifically relates to an Oocystis concentrate with high activity and high stress resistance, and a preparation method and application thereof, belonging to the technical fields of aquaculture water quality regulation and water environment treatment. The preparation method comprises the following steps: isolation and cultivation of purebred Oocystis; seed selection of Oocystis withhigh activity and high stress resistance; enlarged cultivation; industrial cultivation; collection; concentration; and the like, wherein continuous aerated cultivation is carried out under the conditions of a temperature of 23 to 28 DEG C and an illumination of 1800 to 2000 Lx. Compared with the prior art, the concentration of the Oocystis concentrate of the invention can reach 130 to 150 million/ml, can be stored at normal temperature for 95-105 days, has a survival rate of 95% or more, and is convenient for storage, transportation and usage; and the Oocystis concentrate has high activity, high stress resistance and stable algal phase, can keep the water quality of a culture pond in benign equilibrium for a long time, is applicable to preparation of algal phase stabilizers for seawater orbrackish-water culture ponds of marine food products, and shows good industrial application prospects.

Description

technical field [0001] The invention relates to the technical field of aquaculture water quality regulation and control, in particular to a highly active and stress-resistant oocyst concentrate, a preparation method and application thereof. Background technique [0002] Oocystis belongs to Chlorophyta, Trebouxiophyceae, Chlorellales, Oocystaceae. The plant body is a spherical or elliptical spherical group, and the group is usually composed of 2, 4 or 8 cells, and there is a gelatinous coating composed of pectin outside the group. The algae cells are oval or oval, with a length of 10-19 μm and a width of 9-13 μm, and generally produce 2, 4, 8 or 16 spore-like spores. Oocysts are mainly distributed in water bodies rich in organic matter such as estuaries and aquaculture ponds. They have the characteristics of long duration and stable population. They can reduce the concentration of harmful factors such as ammonia nitrogen and nitrite in water bodies, and increase dissolved ox...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/12A01K61/10A01K61/59A01K61/50C12R1/89
CPCA01K61/10A01K61/50A01K61/59C12N1/12Y02A40/81
Inventor 李活
Owner GUANGDONG JINYANG BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap