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Outdoor large-scale expanding culture method for freshwater chlorella vulgaris

A technology of chlorella and fresh water, which is applied in the field of microalgae cultivation, can solve the problems of high production cost, low biomass concentration, and low biomass, and achieve difficult stratification and precipitation, high biomass yield, and oxygen production big effect

Pending Publication Date: 2021-03-26
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Open tank cultivation has the advantages of simple equipment and low production cost, but the growth conditions such as light, temperature, and pH value are uncontrollable, and it is easy to be polluted by foreign organisms and algae, resulting in low biomass and affecting the subsequent use effect and influence to be tested
Although the pipeline bioreactor improves the light-receiving efficiency of chlorella and can control the growth conditions of chlorella, the production cost is high and the maximum biomass concentration is low

Method used

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  • Outdoor large-scale expanding culture method for freshwater chlorella vulgaris
  • Outdoor large-scale expanding culture method for freshwater chlorella vulgaris

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] At 8 o'clock in the morning on a sunny day, choose a new 20L transparent plastic bucket with a matching lid as the cultivation container, put 16L of tap water into it as the cultivation water, cover the lid, and place it in the outdoor sun until 4 o'clock in the afternoon. 2 hours.

[0027] Add the main components of the medium to the culture water after exposure to the sun, and mix well to obtain the medium. The components of the medium and their final concentrations are: sterile organic fertilizer (5g / L), vitamin C (0.005g / L), sulfuric acid Magnesium (60mg / L), citric acid (6mg / L), calcium chloride (30mg / L), dipotassium hydrogen phosphate (40mg / L), zinc sulfate (0.2mg / L), manganese chloride (1.8mg / L L), sodium carbonate (20mg / L), nanocellulose (15mg / L), Rhodopseudomonas palusteris (Rhodops eudomonaspalusteris) (viable bacteria number 10 6 CFU / mL). The sterile organic fertilizer is an organic fertilizer purchased from the market and subjected to high-temperature steam...

Embodiment 2

[0030] At 8 o'clock in the morning on a sunny day, choose a new 5L transparent plastic bucket with a matching lid as the cultivation container, put 4L of tap water into it as the cultivation water, cover the lid, and place it under the sun until 4:30 p.m. more than 2 hours.

[0031] Add the main components of the medium to the culture water after exposure to the sun, and mix well to obtain the medium. The components of the medium and their final concentrations are: sterile organic fertilizer (5g / L), vitamin C (0.005g / L), sulfuric acid Magnesium (60mg / L), citric acid (6mg / L), calcium chloride (30mg / L), dipotassium hydrogen phosphate (40mg / L), zinc sulfate (0.2mg / L), manganese chloride (1.8mg / L L), sodium carbonate (20mg / L), nanocellulose (20mg / L), Rhodopseudomonas palusteris (Rhodops eudomonaspalusteris) (viable bacteria number 10 7 CFU / L).

[0032] Chlorella vulgaris (FACHB-8) was inoculated in the culture medium, the inoculum size per milliliter was 9×10 6 CFU, cultured in...

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Abstract

The invention relates to an outdoor large-scale expanding culture method for fresh water chlorella vulgaris. The method comprises the following steps of inoculating chlorella vulgaris into a culture medium in a transparent culture container for culture, wherein the culture medium is prepared from water, sterile organic fertilizer, vitamin C, magnesium sulfate, citric acid, calcium chloride, dipotassium phosphate, zinc sulfate, manganese chloride, sodium carbonate, nano cellulose and Rhodops eudomonaspalusteris.

Description

technical field [0001] The present disclosure relates to the technical field of microalgae cultivation, in particular, to an outdoor large-scale expansion cultivation method of freshwater chlorella. Background technique [0002] Chlorella (Chlorella vulgaris), Chlorella phylum, Chlorellaceae, often solitary, but also multi-cellular aggregates, spherical or oval, 3-8 microns in diameter. Chlorella is a high-quality green nutrient source food. Its cells are rich in protein, lipids, polysaccharides, vitamins, chlorophyll, trace elements and some biologically active metabolites. It can be used as bait for aquaculture larvae and has the ability to purify water quality And biodiesel extraction raw materials and other functions. At present, the large-scale production of chlorella adopts photoautotrophic methods, mainly including two systems of open pond culture and pipeline bioreactor. Open tank cultivation has the advantages of simple equipment and low production cost, but the g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/20C12R1/89C12R1/01
CPCC12N1/12C12N1/20
Inventor 徐跑陈春光聂志娟徐钢春
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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