Bacillus for biological prevention and control of root rot and bacterial wilt

A technology of Bacillus subtilis and Bacillus, applied in the field of microorganisms, can solve the problems of decline, plant withering and death, yield, and no control measures, etc., and achieve the effects of environmental friendliness, good antagonistic effect, prevention and control of crop diseases

Inactive Publication Date: 2018-05-25
福建省农业科学院农业生物资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease can be called "plant cancer". Once it occurs, it is difficult to control, causing large areas of plant withering and death and a serious decline in yield.
Although there have been many research reports on the control of crop bacterial wilt, such as cultivation measures, crop rotation and variety resistance selection, effective control measures have not yet emerged.

Method used

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  • Bacillus for biological prevention and control of root rot and bacterial wilt
  • Bacillus for biological prevention and control of root rot and bacterial wilt
  • Bacillus for biological prevention and control of root rot and bacterial wilt

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Screening of antagonistic strains

[0018] 1. Soil sample collection and Bacillus isolation and purification

[0019] The grass root soil samples collected from Hoh Xil were air-dried and ground to make a soil suspension, which was then properly diluted with sterile water and coated on an NA plate; cultivated at 30°C for 72 hours, picked a single colony, and preserved it in glycerol as the strain to be tested . 63 strains were isolated and purified by dilution plating method as the strains to be tested.

[0020] 2. Screening of biocontrol strains of Fusarium oxysporum

[0021] (1) Preliminary screening by confrontation method

[0022] The test strain tomato host Fusarium oxysporum FJAT-30512 (preserved in the Institute of Agricultural Biological Resources, Fujian Academy of Agricultural Sciences), in beef extract peptone medium (NA: beef extract 3g, peptone 5g, glucose 10g, agar powder 1.7%, pH 7.2-7.4) on the plate, and incubated at 30°C for 48h. Fusariu...

Embodiment 2

[0040] Identification of embodiment 2 antagonistic strains

[0041] 1. Morphological characteristics

[0042] Bacillus FJAT-19708 was activated on the NA plate, and the colony shape, size, color, transparency, protrusion and edge of the strain were observed after 48 hours.

[0043] The colony characteristics of strain FJAT-19708 are as follows figure 1 As shown, the colonies are small, round, milky white, smooth and moist, raised, with neat edges, and opaque.

[0044] 2. 16S rRNA gene sequence analysis

[0045] The genomic DNA of the strain FJAT-19708 was extracted according to the operation steps of the bacterial genome extraction kit (generay biotech#GK 1071, Shanghai Jierui Bioengineering Co., Ltd.), and the 16S rRNA gene general primers 27F and 1492R were used for PCR amplification. The PCR reaction procedure was referred to Literature by Zheng Xuefang et al. (Zheng Xuefang, Liu Bo, Zhu Yujing, et al. Screening and Identification of Bacillus Bacillus for the Biocontrol ...

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Abstract

The invention provides bacillus for biological prevention and control of root rot and bacterial wilt. The strain is bacillus subtilis FJAT-19708, has a scientific name of Bacillus subtilis FJAT-19708and is preserved in China General Microbiological Culture Collection Center (CGMCC), with the preservation number of CGMCC No. 14431. The bacillus FJAT-19708 screened by the invention has a very strong inhibition effect on main pathogenic bacteria including fusarium oxysporum and fusarium solani of the root rot, and also has a very strong antagonistic effect on ralstonia solanacearum; the bacilluscan be used for preventing and controlling the root rot and also can be used for preventing and controlling the bacterial wilt.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a bacillus strain FJAT-19708 used for biological control of root rot and bacterial wilt. Background technique [0002] Root rot is a systemic infection disease, which is a common soil-borne disease of plants such as lyme, soybean, wheat, tomato, cotton, strawberry, etc. It is an increasingly prominent problem in the United States, Canada, Australia, and China. Due to root rot, the leaves of the plants become smaller, lack luster, the branches are dwarfed, the fruits are thin, or the leaves turn yellow and wilt. In severe cases, the plants gradually die. The main pathogenic populations of root rot are Fusarium oxysporum and Fusarium solani. The control methods include chemical reagents, fungicides, biological control, phytotherapeutic drugs, optimized fertilization, etc., and biocontrol bacteria are used to control Root rot is an important measure for comprehen...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01P1/00A01P3/00C12R1/125
CPCC12N1/205C12R2001/125
Inventor 朱育菁郑梅霞刘波陈峥潘志针
Owner 福建省农业科学院农业生物资源研究所
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