Application of bacillus amyloliquefaciens in tomato cultivation

A technology of amylolytic spores and bacilli, which is applied in the fields of application, fungicide, fruit tree cultivation, etc. It can solve the problems of destroying ecological balance, greatly affecting yield, and yield loss, so as to promote stable yield, improve yield and quality, and increase yield and quality effects

Pending Publication Date: 2022-03-04
天津开发区坤禾生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease can cause fallen leaves, flowers, fruits and broken branches, which has a great impact on yield, and can cause a loss of more than 50% of yield in severe cases
[0004] Chemical control is an effective method to control plant diseases, but the long-term and large-scale use of chemical fungicides will cause environmental pollution such as soil and air, and destroy the ecological balance
With the concern of food safety and environmental pollution in recent years, the use of some chemical fungicides has been strictly restricted in many developed countries and regions

Method used

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  • Application of bacillus amyloliquefaciens in tomato cultivation
  • Application of bacillus amyloliquefaciens in tomato cultivation

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Obtaining isolation and microbial morphology of embodiment 1 bacterial strain

[0021] The Bacillus amyloliquefaciens MES812 of the present invention was obtained in March 2017 by the research and development personnel of our company from the soil of Lycium barbarum in Ning County, Zhongning City, Ningxia. After the soil was diluted, streaked and cultivated at 28°C for 24 hours, the slant strain was made after 5 times of purification, and 2 slant planes were picked and sent to the microbial quality inspection agency of the Ministry of Agriculture for identification, and it was confirmed to be Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) , Stored in refrigerators at 4°C and -80°C.

[0022] Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) MES812 was deposited in China General Microorganism Culture Collection Center (CGMCC) on August 10, 2017, with the preservation number CGMCC NO.14515.

[0023] Bacillus amyloliquefaciens of the present invention is Bac...

Embodiment 2

[0024] The preparation of embodiment 2 bacillus amyloliquefaciens MES812 microbial bacterial agent

[0025] The preparation method of the Bacillus amyloliquefaciens MES812 bacterial agent comprises: primary seed cultivation, fermentation, concentration and spray drying.

[0026] (1) Primary seed culture: The strain MES812 activated on the test tube slant was cultured in an Anbei bottle, and the Anbei bottle medium was a solid nutrient agar medium. The medium formula is: 8-13g of peptone, 2-5g of beef extract, 3-5g of sodium chloride, 19.5g of agar, 1000ml of ultrapure water, and the pH value is 7.0-7.5. The culture conditions are: temperature 25°C-30°C, culture for 24h.

[0027] (2) Fermentation: at 30m 3 The fermenter is equipped with 20L liquid Bacillus amyloliquefaciens fermentation medium, sterilized by moist heat at 115°C-122°C for 35-40min, then cooled to 35°C, scraping the first-grade seed lawn, mixing it with 100mL room temperature sterile water, and inoculating In ...

Embodiment 3

[0032] Example 3 Antibacterial test of bacillus amyloliquefaciens MES812 on tomato early blight Alternaria solani

[0033] (1) Test time and place: In March 2021, it will be conducted in the laboratory of the company's R&D center.

[0034] (2) Test method:

[0035] (1) The source of the tested bacteria

[0036] The pathogen of tomato early blight comes from the tomato farmland in Yunnan. It was cultivated, isolated and purified by the R&D personnel of the company. It was finally identified as Alternaria solani by 16SrDNA, and it was identified as strong pathogenicity by pathogenicity testing.

[0037] Bacillus amyloliquefaciens is prepared from the strain described in Example 2 through primary seed culture, fermentation culture, and powder spraying.

[0038] (2) Plate antagonism experiment

[0039] Prepare a PDA solid plate, pick an Alternaria bacterium with a sterile inoculation needle and place it in the middle of the petri dish, burn the inoculation needle red, dip the B...

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Abstract

The invention belongs to the technical field of prevention and control of soil-borne plant diseases by microorganisms, and particularly relates to application of bacillus amyloliquefaciens MES812 in tomato cultivation, the strain is bacillus amyloliquefaciens MES812, the preservation date is August 10, 2017, the preservation unit is China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.14515. The invention further discloses a preparation method of the bacillus amyloliquefaciens MES812. The strain provided by the invention has an obvious antagonistic effect on alternaria solani causing tomato early blight, can prevent or alleviate the occurrence of tomato early blight, promotes stable yield and income increase of tomatoes, and improves and enhances the yield and quality of tomatoes.

Description

technical field [0001] The invention belongs to the technical field of using microorganisms to prevent and control soil-borne plant diseases, and in particular relates to the application of bacillus amyloliquefaciens in tomato cultivation. Background technique [0002] Tomato Early Blight (Tomato Early Blight), also known as "Ring Blight", occurs widely in various places and is one of the important diseases that harm tomato. For a long time, due to the promotion of tomato varieties that are resistant to viral diseases but not resistant to early blight in some areas, early blight has seriously occurred. This pathogen has a wide range of hosts. In addition to harming tomatoes, it can also harm eggplants, peppers and other solanaceous vegetable crops and potatoes. [0003] Tomato early blight is a worldwide disease caused by Alternaria solani Sorauer, which mainly destroys the cell wall, cytoplasm, cell membrane, mitochondria, chloroplast of some metabolic enzymes of the host ...

Claims

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Application Information

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IPC IPC(8): A01N63/22A01P3/00A01G22/05
CPCA01N63/22A01G22/05
Inventor 李立培唐广辉赵钢勇王焱冯美元
Owner 天津开发区坤禾生物技术有限公司
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