Application of bacillus amyloliquefaciens in tomato cultivation
A technology of amylolytic spores and bacilli, which is applied in the fields of application, fungicide, fruit tree cultivation, etc. It can solve the problems of destroying ecological balance, greatly affecting yield, and yield loss, so as to promote stable yield, improve yield and quality, and increase yield and quality effects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0020] Obtaining isolation and microbial morphology of embodiment 1 bacterial strain
[0021] The Bacillus amyloliquefaciens MES812 of the present invention was obtained in March 2017 by the research and development personnel of our company from the soil of Lycium barbarum in Ning County, Zhongning City, Ningxia. After the soil was diluted, streaked and cultivated at 28°C for 24 hours, the slant strain was made after 5 times of purification, and 2 slant planes were picked and sent to the microbial quality inspection agency of the Ministry of Agriculture for identification, and it was confirmed to be Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) , Stored in refrigerators at 4°C and -80°C.
[0022] Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) MES812 was deposited in China General Microorganism Culture Collection Center (CGMCC) on August 10, 2017, with the preservation number CGMCC NO.14515.
[0023] Bacillus amyloliquefaciens of the present invention is Bac...
Embodiment 2
[0024] The preparation of embodiment 2 bacillus amyloliquefaciens MES812 microbial bacterial agent
[0025] The preparation method of the Bacillus amyloliquefaciens MES812 bacterial agent comprises: primary seed cultivation, fermentation, concentration and spray drying.
[0026] (1) Primary seed culture: The strain MES812 activated on the test tube slant was cultured in an Anbei bottle, and the Anbei bottle medium was a solid nutrient agar medium. The medium formula is: 8-13g of peptone, 2-5g of beef extract, 3-5g of sodium chloride, 19.5g of agar, 1000ml of ultrapure water, and the pH value is 7.0-7.5. The culture conditions are: temperature 25°C-30°C, culture for 24h.
[0027] (2) Fermentation: at 30m 3 The fermenter is equipped with 20L liquid Bacillus amyloliquefaciens fermentation medium, sterilized by moist heat at 115°C-122°C for 35-40min, then cooled to 35°C, scraping the first-grade seed lawn, mixing it with 100mL room temperature sterile water, and inoculating In ...
Embodiment 3
[0032] Example 3 Antibacterial test of bacillus amyloliquefaciens MES812 on tomato early blight Alternaria solani
[0033] (1) Test time and place: In March 2021, it will be conducted in the laboratory of the company's R&D center.
[0034] (2) Test method:
[0035] (1) The source of the tested bacteria
[0036] The pathogen of tomato early blight comes from the tomato farmland in Yunnan. It was cultivated, isolated and purified by the R&D personnel of the company. It was finally identified as Alternaria solani by 16SrDNA, and it was identified as strong pathogenicity by pathogenicity testing.
[0037] Bacillus amyloliquefaciens is prepared from the strain described in Example 2 through primary seed culture, fermentation culture, and powder spraying.
[0038] (2) Plate antagonism experiment
[0039] Prepare a PDA solid plate, pick an Alternaria bacterium with a sterile inoculation needle and place it in the middle of the petri dish, burn the inoculation needle red, dip the B...
PUM
Property | Measurement | Unit |
---|---|---|
Radius | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com