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Antibacterial activity of trichoderma aureoviride and application of trichoderma aureoviride

A technology of Trichoderma chrysanthemum and antibacterial activity, applied in the direction of application, fungicide, fungi, etc., can solve the problem of drug resistance of environmental pollution pathogenic bacteria, and achieve good control effect, good antagonism, and good chitinase activity Effect

Active Publication Date: 2021-08-27
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] In recent years, the extensive use of chemical pesticides has caused environmental pollution and drug resistance of pathogenic bacteria

Method used

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  • Antibacterial activity of trichoderma aureoviride and application of trichoderma aureoviride
  • Antibacterial activity of trichoderma aureoviride and application of trichoderma aureoviride
  • Antibacterial activity of trichoderma aureoviride and application of trichoderma aureoviride

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Embodiment Construction

[0026] 1. the preparation of the chitinase of Trichoderma chrysogreen QTYC44 of the present invention and the enzyme activity assay method comprise the steps:

[0027] (1) Activation: Inoculate Trichoderma chrysanthemum QTYC44 with the preservation number CCTCC M 2014267 on PDA solid medium, and activate and culture at 28°C for 4-7 days to obtain an activated strain;

[0028] (2) Inoculate the activated bacterial strain prepared in step (1) into MYG liquid medium for fermentation, and culture it for 3-4 days at 28°C and pH 7.0 to obtain seed liquid;

[0029] (3) inoculating the seed solution prepared in step (2) into a chitinase-inducing medium for fermentation, and culturing for 6-9 days at 28°C and pH 7.0 to obtain a fermentation solution;

[0030] (4) Take the fermented liquid prepared in step (3), centrifuge it at 8000r / min for 10min, and take its supernatant every day on the 3rd to 9th day as the crude enzyme liquid.

[0031] Add 0.5 mL of crude enzyme solution, 1.5 mL o...

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Abstract

The invention discloses antibacterial activity of trichoderma aureoviride and an application of the trichoderma aureoviride, and the trichoderma aureoviride is a trichoderma aureoviride QTYC44 which is preserved in the China Center for Type Culture Collection (CCTCC) and has the preservation number of CCTCC M 2014267. The trichoderma aureoviride provided by the invention has a very good bacteriostatic effect on fusarium wilt of cucumber, gibberella zeae and alternaria alternata, has a very good prevention and treatment effect on fusarium wilt of cucumber, has relatively good chitinase enzymatic activity, and can be used for preparing a biocontrol microbial inoculum and preventing and treating fungal diseases.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of antibacterial activity of a Trichoderma sp. QTYC44 strain. Background technique [0002] Trichoderma (Trichoderma spp.) fungi belong to the subphylum Deuteromycotina, Hyphomycetes, Hyphospora, Polychodermaceae, and Trichoderma. Trichoderma fungi are rich in species and widely exist in the environment such as soil and around plants. Trichoderma is a multifunctional environment-friendly fungus, which is widely used in agriculture, plant protection, environmental protection and other fields. [0003] In the present invention, Trichoderma chrysopride comes from the intestinal tract of the larvae of the yellow dragon. The intestinal tract of insects is the most important colonization site for microorganisms. A series of characteristics of the intestinal tract of insects are conducive to the colonization of microorganisms, including the colonization of microorganisms re...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N9/42A01N63/38A01P3/00C12R1/885
CPCC12N1/14C12N9/2442A01N63/38C12Y302/01014
Inventor 张应烙刘丽君邵明伟尹彩萍
Owner ANHUI AGRICULTURAL UNIVERSITY
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