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A kind of antibacterial activity of Trichoderma and its application

A technology of Trichoderma and wheat gibberella, applied in the direction of application, fungicides, fungi, etc., can solve environmental pollution pathogenic bacteria resistance and other problems, achieve good control effect, good antagonistic effect, good chitinase active effect

Active Publication Date: 2022-04-12
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, the extensive use of chemical pesticides has caused environmental pollution and drug resistance of pathogenic bacteria

Method used

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  • A kind of antibacterial activity of Trichoderma and its application
  • A kind of antibacterial activity of Trichoderma and its application
  • A kind of antibacterial activity of Trichoderma and its application

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Embodiment Construction

[0026] 1. the preparation of the chitinase of Trichoderma QTYC44 of the present invention and enzyme activity assay method, comprise the steps:

[0027] (1) Activation: Trichoderma QTYC44 with the preservation number CCTCC M 2014267 was inoculated on PDA solid medium, and activated and cultured at 28°C for 4-7 days to obtain an activated strain;

[0028] (2) Inoculate the activated bacterial strain prepared in step (1) into MYG liquid medium for fermentation, and culture for 3-4 days at 28°C and pH 7.0 to obtain seed liquid;

[0029] (3) Inoculate the seed solution prepared in step (2) into a chitinase-inducing medium for fermentation, and culture for 6-9 days at 28°C and pH 7.0 to obtain a fermentation solution;

[0030] (4) Take the fermented liquid prepared in step (3), centrifuge it at 8000r / min for 10min, and take its supernatant every day on the 3rd to 9th day as the crude enzyme liquid.

[0031] Add 0.5 mL of crude enzyme solution, 1.5 mL of 0.5 mM pH7.8 phosphate buff...

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Abstract

The invention discloses the antibacterial activity of Trichoderma chrysoviide and its application. The Trichoderma chrysoviide of the invention is Trichoderma chrysoviide QTYC44 with the preservation number CCTCC M 2014267 preserved by the China Center for Type Culture Collection. The Trichoderma chrysanthemum of the present invention has good antibacterial effect on cucumber wilt, wheat gibberella and tobacco Alternaria Alternaria, has good control effect on cucumber wilt, and has better chitinase enzyme activity, can Preparation of biocontrol agents to prevent and control fungal diseases.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of antibacterial activity of a Trichoderma sp. QTYC44 strain. Background technique [0002] Trichoderma (Trichoderma spp.) fungi belong to the subphylum Deuteromycotina, Hyphomycetes, Hyphospora, Polychodermaceae, and Trichoderma. Trichoderma fungi are rich in species and widely exist in the environment such as soil and around plants. Trichoderma is a multifunctional environment-friendly fungus, which is widely used in agriculture, plant protection, environmental protection and other fields. [0003] The trichoderma of the present invention comes from the gut of the larvae of the yellow dragonfly. The insect gut is the most important colonization place for microorganisms. A series of characteristics of the insect gut are conducive to the colonization of microorganisms, including the colonization of microorganisms related to feeding. The nutrient availability is stro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N9/42A01N63/38A01P3/00C12R1/885
CPCC12N1/14C12N9/2442A01N63/38C12Y302/01014
Inventor 张应烙刘丽君邵明伟尹彩萍
Owner ANHUI AGRICULTURAL UNIVERSITY
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