Novel sand fixing method of terraneous nitrogen fixation blue algae
A technology of nitrogen-fixing cyanobacteria and a new method, which is applied in the field of sand-fixing biology and sand control in arid areas, and can solve the problems of large investment and environmental pollution of chemical sand-fixing agents
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[0011] a. Cyanobacteria cultivation: The algae used is the existing Scytonema ocellatum Lynbye, which belongs to the genus Scytonema Ag. of the family Scytonemataceae, and is isolated from Gurbantunggut, Xinjiang. The natural desert crust of the desert is carried out in a conventional light cultivation room in the form of liquid or solid, and is cultured and separated by conventional IAM collection medium to obtain a pure culture;
[0012] b. For the expanded cultivation of algae species, use De medium for single species culture, inoculate the pure culture of algae species, the culture temperature is 25-28 ° C, and the light intensity is 3500-8000 lux;
[0013] c. Inoculation environment: Sandy soil, annual precipitation ≥ 100mm, and winter snowfall of 15-20cm disturbed sand dune activation area to take sand samples, use a Φ1mm sieve to remove large particles of debris, 15Mpa, 50min damp heat sterilization, inoculation of nitrogen-fixing cyanobacteria culture Illuminated by na...
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