A method of using spray drying for preparing probiotics micro-capsules

A technology of probiotics and microcapsules, applied in applications, animal feed, animal feed, etc., can solve the problems of poor storage stability, low survival rate, and poor acid resistance, and achieve good encapsulation performance, easy-to-obtain wall materials, and low prices Effect

Inactive Publication Date: 2013-09-11
GUANGZHOU GLAM BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0020] (1) Probiotics such as Lactobacillus and Bifidobacterium are mostly anaerobic bacteria or facultative anaerobic bacteria. During the growth process, they do not form spores and have poor resistance. Most of them have poor acid resistance and are extremely sensitive to oxygen. It is more difficult to maintain, so that the number of viable bacteria in commercially available products decreases rapidly, the storage stability of the products is extremely poor, and the health care efficacy loses quickly;
[0021] (2) For probiotics to have a beneficial effect on the body, they must first pass through the gastric environment and reach the intestinal tract with a large number of survi...

Method used

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  • A method of using spray drying for preparing probiotics micro-capsules
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Embodiment 1

[0040] Embodiment 1 A method for preparing Lactobacillus acidophilus microcapsules by spray drying, comprising the following steps:

[0041] (1) Activation and value-added of strains:

[0042] Introduce the activated Lactobacillus acidophilus into the MRS medium, and cultivate it to the logarithmic growth phase by conventional fermentation method at constant temperature, about 1.13×10 9 CFU / mL, put the suspension of Lactobacillus acidophilus as the core material in the refrigerator at 4°C for later use;

[0043] (2) Preparation of mixed suspension:

[0044] The ratio of gum arabic and maltodextrin is 1:10 as the wall material, dissolved in deionized water at a concentration of 20%, and prepared into a stable, uniform, and fluid wall material solution, and Lactobacillus acidophilus is suspended at room temperature. Add an equal volume of liquid into the wall material solution, stir thoroughly, and mix evenly;

[0045] (3) Spray drying: the air inlet temperature is...

Embodiment 2

[0050] Embodiment 2 A kind of method adopting spray drying to prepare Lactobacillus casei microcapsules comprises the following steps:

[0051] (1) Activation and value-added of strains:

[0052] Insert the activated Lactobacillus casei strain into the MRS medium, and cultivate it to the logarithmic growth phase at a constant temperature by conventional fermentation method, about 1.52×10 9 CFU / mL, the suspension of Lactobacillus casei was absorbed by 6% corn porous starch as the core material and put in the refrigerator at 4°C for later use;

[0053] (2) Preparation of mixed suspension:

[0054] The ratio of gum arabic and maltodextrin is 1:8 as the wall material, dissolved in deionized water at a concentration of 18%, and prepared into a stable, uniform, and fluid wall material solution. The core material adsorbed by starch is added to the wall material solution according to the volume ratio of 1:2, fully stirred, and mixed evenly;

[0055] (3) Spray drying: the ai...

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Abstract

The present invention relates to a method of using spray drying for preparing probiotics micro-capsules, belonging to the technical field of micro-organisms. According to the method, a probiotic suspension or probiotics adsorbed in porous starch is used as a core material, gum arabic and maltodextrin are used as a wall material, and the probiotics micro-capsules are prepared through a spray drying process. The method comprises the steps of: arabic gum and maltodextrin at a ratio of 1:7 to 1:10 as the wall material, at a concentration of 15 to 20% dissolved in deionized water, mixing uniformly with the probiotic suspension or the probiotics adsorbed in porous starch at room temperature; and spray drying under conditions of a inlet air temperature being 130-150DEG C and a feeding rate being 12.50-15.50mL/min, wherein skim milk powder is adopted as a heat-resistant protective agent in an amount of 1 to 3%, and embedding rate of the probiotics is above 85% by using the method of the invention. The method has the advantages of being fast in drying rate, short in time, low in material temperature, good in product dispersion and solubility, simple in production process, stable in effect, long in shelf life of products, and suitable for continuous production.

Description

technical field [0001] The invention relates to a method for preparing probiotic microcapsules by spray drying, which maintains the activity of probiotics and is used as a feed additive for livestock and poultry breeding. [0002] Background technique [0003] Probiotics, also known as probiotics, probiotics, active bacteria, and microecological preparations, refer to live bacterial products or bacterial components that maintain the balance of human flora and have beneficial effects on human health. and dead bacteria products of metabolites. The types of probiotics currently used in humans mainly include Lactobacillus, Bifidobacterium, Enterococcus, Lactococcus, Bacillus subtilis, Escherichia coli, Bacillus cereus, Bacillus licheniformis, Streptococcus thermophilus, Clostridium butyricum and Yeast, etc., but traditionally people use mainly two genera of Bifidobacterium and Lactobacillus. In commercial production and application, especially in food, Lactobacillus acidophil...

Claims

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Application Information

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IPC IPC(8): A23K1/16
Inventor 李帅伟李本涛
Owner GUANGZHOU GLAM BIOTECH
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