Trichoderma LJ245 capable of degrading aureomycin

A technology of LJ245 and chlortetracycline, which is applied in the field of microorganisms, can solve the problems that have not been reported, and achieve the effect of high acid resistance and rapid growth

Inactive Publication Date: 2012-07-25
BEIJING INSTITUTE OF TECHNOLOGYGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The bacterial strain LJ245 involved in this patent is Trichoderma harzianum (Hypocrea lixii), and the research on ...

Method used

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  • Trichoderma LJ245 capable of degrading aureomycin
  • Trichoderma LJ245 capable of degrading aureomycin
  • Trichoderma LJ245 capable of degrading aureomycin

Examples

Experimental program
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Effect test

Embodiment 1

[0029] One, the screening method of a chlortetracycline degrading bacterial strain LJ245

[0030] 1. Material preparation

[0031] Source of bacteria samples: collected from wastewater containing activated sludge from aureomycin production enterprises.

[0032] Medium:

[0033] Inorganic salt solid medium: (NH4) 2 SO 4 2.0g, K 2 HPO 4 0.5g, KH 2 PO 4 0.5g, MgSO 4 7H2O 0.5g, NaCl 0.2g, CaCl 2 0.1g, FeSO 4 0.01g, 0.015g EDTA, 13g agar, 2g / L glucose, dissolved in 1000ml distilled water. After damp heat sterilization at 115°C for 20 minutes, 2.0 g / L aureomycin was added.

[0034] Martin's medium: peptone 6g, glucose 10g, KH 2 PO 4 1.0g, MgSO 4 ·7H2O 0.5g, dissolved in 1000ml of distilled water. Sterilize with damp heat at 115°C for 20 minutes.

[0035] Activity assay medium: (NH4) 2 SO 4 2.0g, K 2 HPO 4 0.5g, KH 2 PO 4 0.5g, MgSO 4 7H2O 0.5g, NaCl 0.2g, CaCl 2 0.1g, FeSO 4 0.01g, 0.015g of EDTA, dissolved in 1000ml of distilled water. After damp hea...

Embodiment 2

[0077] Utilization and degradation of aureomycin as the only carbon source by strain LJ245

[0078] The preparation uses aureomycin as the only carbon source and the concentration of aureomycin is 0.01g / L, 0.05g / L, 0.10g / L, 0.50g / L, 5.00g / L, 10.00g / L, 15.00g / L inorganic salt solid medium. Pick a few LJ245 colonies, streak on the inorganic salt solid medium plate, and culture at 30°C in the dark for 2-6 days. It was observed that on the second day of cultivation, bacteria were grown on the medium plates with aureomycin concentration of 0.01g / L, 0.05g / L, 0.1g / L, 0.5g / L and 5.0g / L. silk and grow well. It shows that the strain LJ245 can use and degrade aureomycin at a very low concentration of 0.01g / L to a medium concentration of 5.0g / L. Strain LJ245 can utilize aureomycin at a low concentration or even an oligotrophic concentration, which can minimize the residual amount of aureomycin.

[0079] The strain LJ245 was inserted into the inorganic salt liquid medium with chlortetr...

Embodiment 3

[0081] Experiment on Treatment of Chlortetracycline Wastewater by Strain LJ245

[0082] The strain LJ245 was activated and cultured with Martin's medium, and then added to the production wastewater of aureomycin enterprise according to the inoculum amount of 1%, and the original wastewater not inoculated with microorganisms was used as a control, and placed in a shaker at 28°C and 170r / min respectively. Cultivate in the bed for 4 days, and determine the residual amount of aureomycin in the waste water. It was determined that the aureomycin removal rate of the inoculated strain LJ245 was increased by 36.38% compared with the control.

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Abstract

The invention relates to Trichoderma LJ245 capable of degrading aureomycin, belonging to the technical field of the microorganism. The 16S rDNA full sequence determination shows that: the strain LJ245 is Hypocrea lixii with the preservation number CGMCC No.5753. The strain LJ245 grows fast; mycelium is in the white flocculence and at the late growth stage, the mycelium is green yellow due to generation of conidium. The microscopic observation shows that: the mycelium is septate, colorless conidiophore vertically grows out and the conidium is spherical. The strain LJ245 has the function of degrading the aureomycin remained in the strain dregs and waste water, is also applied in treating the wastes from the aureomycin production enterprise, is also expected to be applied on the biological repair of the aureomycin polluted environment, especially is used as a sole carbon source, moreover the strain still grows well when the concentration of the aureomycin is low to 0.01g/L, thus the residual aureomycin is reduced to the hilt. Simultaneously, the strain is conveniently used and also used as the preparation raw material of an aureomycin degradation strain agent.

Description

technical field [0001] The invention relates to a strain of Trichoderma LJ245 capable of degrading aureomycin, belonging to the technical field of microorganisms. Background technique [0002] Aureomycin is a broad-spectrum antibiotic mainly produced in my country. A large amount of chlortetracycline residue and waste water will be produced during the fermentation and production of chlortetracycline. Due to the presence of aureomycin residues in the fungus residue, it has been included in the "National List of Hazardous Wastes" by the state and can only be disposed of by incineration and landfill. Incineration consumes a lot of energy, and the combustion gas may cause secondary pollution. Landfilling requires a large amount of land, and there is a potential risk of leakage of antibiotic substances, which poses a potential hazard to the environment and human health. If the residual chlortetracycline in the wastewater is discharged into the surrounding water and soil, it wi...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N15/11C02F3/34A62D3/02C12R1/645C02F101/38A62D101/28
Inventor 李艳菊肖思颖杨正礼杨世琦张晴雯张爱平
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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