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Chemiluminescence detection kit of aureomycin and preparation method thereof

A chemiluminescence detection and chemiluminescence technology, which is applied in chemiluminescence/bioluminescence, biological testing, and analysis through chemical reactions of materials, can solve problems such as large error in results, low sensitivity, and poor specificity of microbial methods, and achieve The photon yield does not decrease, the reagent stability is good, and the effect of less interference factors

Inactive Publication Date: 2018-01-05
太原瑞盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The specificity of the microbiological method is poor, the detection period is long and the result error is large
CN 1811435 A (August 2006) discloses a kit for detecting aureomycin by enzyme-linked immunoassay. The disadvantage of using enzyme-linked immunoassay for detection is that horseradish peroxidase or alkaline phosphate markers are easily inactivated, The chromogenic substrate is easy to decompose when exposed to light, has low sensitivity, and has a certain cross-reaction to compounds with similar structures, resulting in inaccurate test results, and is only suitable for the detection and identification of trace substances
CN 103512974 A (January 2014) discloses a method for detecting chlortetracycline by high-performance liquid chromatography. The disadvantage of using high-performance liquid chromatography is that the sample pretreatment is complicated, laborious and time-consuming, and the detection cost is high
CN 102020713 A (April 2011) discloses a kit for detecting chlortetracycline with colloidal gold. The disadvantage of using the colloidal gold method for detection is that the final detection result is characterized by a color visible to the naked eye, and the error is relatively large, and the operation It is cumbersome, has many processes, is more prone to errors, and has low sensitivity

Method used

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preparation example Construction

[0023] The invention provides a chlortetracycline chemiluminescence detection kit and a preparation method, wherein the chemiluminescence detection kit provided by the present invention can use aureomycin antibody-coupled magnetic particles, acridinium ester To label aureomycin, you can also use aureomycin antigen-coupled magnetic particles, and acridinium ester-labeled aureomycin antibody. The kit also includes chlortetracycline calibrator, chemiluminescence pre-excitation solution A, chemiluminescence excitation solution B and cleaning solution.

[0024] Specifically, in the process of preparing magnetic particle coupling in the present invention, the buffer solution for coupling antigen is a buffer solution with a pH value of 5.0 and a concentration of 0.1 mol / L MES; the buffer solution for coupling antibody is a buffer solution with a pH value of 5.0 6.0. Buffer solution with a concentration of 0.1 mol / L MES.

[0025] Specifically, in the process of preparing the magnetic...

Embodiment 1

[0031] Embodiment 1: The composition and preparation method of a chlortetracycline chemiluminescent detection kit 1

[0032] 1. Assembly of Kit 1

[0033] Aureomycin antigen labeled with acridinium ester;

[0034] Aureomycin antibody coupled to magnetic particles;

[0035] Chlortetracycline series calibrator solutions, concentrations are: 0 μg / L, 0.01 μg / L, 0.1 μg / L, 1 μg / L, 10 μg / L, 100 μg / L;

[0036] Chemiluminescence pre-excitation solution A and chemiluminescence excitation solution B;

[0037] Cleaning solution, including the following components: Tris-HCl with a pH of 7.2 and a concentration of 25 mmol / L, which contains NaCl and 0.05% Tween-20 with a concentration of 0.15 mmol / L.

[0038] 2. Preparation of Magnetic Microparticle Suspension Conjugated with Chlortetracycline Antibody

[0039](1) Take 1 mg of carboxyl magnetic particles in a 0.5 mL centrifuge tube, add a certain amount of 0.1 mol / L MES buffer, vortex and mix, place on a magnetic stand, and let it stand ...

Embodiment 2

[0056] Embodiment 2: The formation and preparation method of a chemiluminescence detection kit 2 of aureomycin

[0057] 1. Assembly of Kit 2

[0058] Acridinium ester-labeled aureomycin antibody;

[0059] Aureomycin antigen coupled to magnetic particles;

[0060] Chlortetracycline series calibrator solutions, concentrations are: 0 μg / L, 0.01 μg / L, 0.1 μg / L, 1 μg / L, 10 μg / L, 100 μg / L;

[0061] Chemiluminescence pre-excitation solution A and chemiluminescence excitation solution B;

[0062] Cleaning solution, including the following components: Tris-HCl with a pH of 7.2 and a concentration of 25 mmol / L, which contains NaCl and 0.05% Tween-20 with a concentration of 0.15 mmol / L.

[0063] 2. Preparation of Magnetic Microparticle Suspension Conjugated with Chlortetracycline Antigen

[0064] (1) Take 1 mg of carboxyl magnetic particles in a 0.5 mL centrifuge tube, add a certain amount of 0.1 mol / L MES buffer, vortex and mix, place on a magnetic stand, and let it stand for 5 minu...

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Abstract

The invention discloses a chemiluminescence detection kit of aureomycin and a preparation method thereof. The kit comprises the following components: an acridinium ester labelled aureomycin antigen orantibody, a magnetic particle coupled antibody or antigen, an aureomycin series standard substance solution, chemiluminescence preexcitation liquid A, chemiluminescence preexcitation liquid B, and cleaning fluid. The kit combines chemiluminescence technology and immunomagnetic particles, and provides a reaction system approaching to a homogeneous phase. Compared with the prior art, the kit has the advantages of high sensitivity, good specificity, short reaction time, and the like.

Description

technical field [0001] The invention belongs to the field of food safety detection, in particular to a chlortetracycline immunochemiluminescence detection kit and a preparation method thereof. Background technique [0002] Chlortetracycline, also known as chlortetracycline, is similar to tetracycline and is also a spectrum antibacterial drug. As an additive in dairy products, honey, organic fertilizers, etc., it can prevent and treat diseases caused by bacteria, chlamydia, and mycoplasma, and has a good antibacterial and antibacterial effect. In the long-term treatment practice, it has been found that aureomycin is a good antibacterial drug, but if this type of antibiotic is often abused, the parasite will acquire stronger drug resistance than before. In addition, after these antibiotics are enriched in animals, they will enter the human body through the food chain and endanger human health. The products exported by my country have also been boycotted by the European Union...

Claims

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Application Information

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IPC IPC(8): G01N33/58G01N33/543G01N21/76
Inventor 曹晶刘丽青胡雪婷常燕杜爱铭徐兵
Owner 太原瑞盛生物科技有限公司
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