A fermentation production method for aureomycin by utilizing mycoprotein in place of a portion of yeast powder

Abstract

The invention provides a technology method used in an aureomycin fermentation production process by utilizing mycoprotein in place of a portion of yeast powder. The mycoprotein is added into an aureomycin basal culture medium according to a certain ratio to replace the portion of the yeast powder, thus achieving an objective of reducing the production cost of aureomycin. The method is characterized in that culture medium aqueous solution per liter in a fermentation tank in a fermentation preparation process comprises following components: 20 g of groundnut meal, 10 g of soybean cake powder, 60 g of the corn starch, 0.1 g of amylase, 2 g of sodium chloride, 7.3 g of ammonium sulfate, 5.7 g of calcium carbonate, 4.35 g of the yeast powder, 4.35 g of the mycoprotein, 13.3 g of corn steep liquor, 0.22 g of magnesium sulfate, 0.1 g of potassium dihydrogen phosphate and 0.17 g of soybean oil. When the basal culture medium sterilization is completed and the temperature is decreased to 30-35 DEG C, a second-stage seed is transferred into the fermentation tank. After seed transference, fermentation production is controlled by changing temperature. The culture pressure is controlled at 0.01-0.04 MPa. The fermentation culture viscosity is controlled at 1-35 s. The fermentation period is 90-124 h. According to the method, the mycoprotein is adopted in the fermentation process to replace 50% of the yeast powder, the production is almost the same as that of common fermentation, products are qualified and the cost of raw materials for one tank is reduced by 1040 Yuan, and therefore the cost of the raw materials is reduced in the case of guaranteeing production stability.

Description

technical field [0001] The invention relates to a method for fermenting aureomycin, in particular to a method for fermenting aureomycin by using cell protein to replace part of yeast powder, and belongs to the field of raw material replacement in fermenting aureomycin. Background technique [0002] The fermentative production method of aureomycin is to use Streptomyces aureus as the starting strain, through pure culture and three-stage fermentation to generate aureomycin. Spore preparation process, primary seed preparation process, secondary seed preparation process, fermentation preparation process. In the fermentation preparation process, each liter of medium aqueous solution in the fermenter contains: peanut cake powder 20g, soybean cake powder 10g, corn starch 60g, amylase 0.1g, sodium chloride 2g, ammonium sulfate 7.3g, calcium carbonate 5.7g, Yeast powder 4.35g, bacterial protein 4.35g, corn steep liquor 13.3g, magnesium sulfate 0.22g, potassium dihydrogen phospha...

AI Technical Summary

Problems solved by technology

[0004] Yeast powder is one of the commonly used nitrogen sources for fermentation. Compared with other organic nitrogen sources, its price is relatively high, resulting in high raw material costs

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