OAP-MIP multiple binding site affinity membrane chlortetracycline (CTC) sensor and preparation method thereof

Abstract

The invention relates to a chlortetracycline (CTC) molecularly imprinted (MIP) affinity membrane sensor and a preparation method thereof. The specific affinity membrane is formed based on an electropolymerization method, and CTC as a template molecule and o-aminophenol (OAP) as a functional monomer form a molecularly imprinted polymer membrane on the surface of a glassy carbon electrode by an electrochemistry method. CTC contains a phenolic hydroxyl group, an enol group and a dimethylamino group and the hydroxyl group in the OAP structure can be used as a latent coordination site so that OAP amino group, and phenolic hydroxyl group and enol group of CTC undergo intermolecular hydrogen bond interaction. OAP hydroxyl group and CTC dimethylamino group undergo a reaction so that multiple binding sites between OAP and CTC exist and the prepared molecularly imprinted polymer has very high CTC selectivity and bonding capability. The sensor can be used for on-site fast detection, does not need specimen pre-treatment, and has a low cost, simple processes and high sensitivity. The sensor has a detection linear range of 20-610nM and sensitivity of 15nM.

Description

technical field [0001] The invention belongs to the field of electrochemical analysis of antibiotic residues, and relates to molecular imprinting technology, in particular to an aureomycin molecular imprinting sensor and a preparation method thereof. Background technique [0002] Chlortetracyline (CTC) is a kind of tetracycline spectrum antibiotics, which can be extracted from Streptomyces culture fluid or produced by semi-synthetic method, and can effectively inhibit Gram-positive bacteria, negative bacteria, Rickettsia, etc. Wide range of antibacterial. It can be widely used in the prevention and treatment of fish and poultry diseases. Adding it to feed can increase the daily weight gain and feed conversion rate of poultry and livestock. However, due to drug abuse, aureomycin residue in animal food constitutes a potential hazard to human health. Most countries have made strict restrictions on the residues of aureomycin in animal foods. The No. 235 document "The Maximum R...

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Problems solved by technology

[0003] In recent years, the commonly used methods of detecting aureomycin that have been reported at home and abroad include high performance liquid chromatography (HPLC), immunoassay, capillary electrophoresis, thin layer chromatography (TLC), microbial culture method, wherein, HPLC method Although it is the most widely used and the test results are accurate and reliable, it requires high sample purity and cannot meet the needs of rapid screening of large batches of samples.

Immunoassay does not require high sample purity, but the reproducibility of test results is poor

Capillary electrophoresis and TLC analysis are fast, but low sensitivity

The microbial culture method is suitable for screening a large number of samples, but cannot be quantitatively analyzed due to the impact of antibacterial active impurities

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