OAP-MIP multiple binding site affinity membrane chlortetracycline (CTC) sensor and preparation method thereof

An affinity membrane chlortetracycline and binding site technology, applied in the field of electrochemical analysis of antibiotic residues, can solve the problems of inability to meet the rapid screening of large-scale samples, poor reproducibility of test results, and high requirements for sample purity, and achieve good results. Electrochemical stability, low cost, good selectivity

Inactive Publication Date: 2015-11-25
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

[0003] In recent years, the commonly used methods of detecting aureomycin that have been reported at home and abroad include high performance liquid chromatography (HPLC), immunoassay, capillary electrophoresis, thin layer chromatography (TLC), microbial culture method, wherein, HPLC method Although it is the most widely used and the test results are accurate and reliable, it requires high sample purity an

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  • OAP-MIP multiple binding site affinity membrane chlortetracycline (CTC) sensor and preparation method thereof
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  • OAP-MIP multiple binding site affinity membrane chlortetracycline (CTC) sensor and preparation method thereof

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Embodiment Construction

[0025] The method for detecting a trace amount of aureomycin residue based on a molecularly imprinted sensitive membrane sensor of the present invention comprises the preparation of the aureomycin molecularly imprinted sensitive membrane sensor and the drawing of an electrochemical response and a standard curve.

[0026] 1. Preparation of aureomycin molecularly imprinted sensitive membrane sensor

[0027] (1) Pretreatment of glassy carbon electrodes: the glassy carbon electrodes are sequentially treated with d-Al 2 o 3The polishing powder is ground and polished, and then ultrasonically cleaned with 1:1 HNO3, absolute ethanol and double distilled water for 2 to 3 minutes respectively, and then the electrode is placed in a 0.5mol / L H2SO4 solution and activated by cyclic voltammetry. The scanning range is -0.1~ 1.0V, scan repeatedly until a cyclic voltammetry (CV) diagram with symmetrical and stable cathode and anode peaks is obtained. After reaching stability, the peak potenti...

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Abstract

The invention relates to a chlortetracycline (CTC) molecularly imprinted (MIP) affinity membrane sensor and a preparation method thereof. The specific affinity membrane is formed based on an electropolymerization method, and CTC as a template molecule and o-aminophenol (OAP) as a functional monomer form a molecularly imprinted polymer membrane on the surface of a glassy carbon electrode by an electrochemistry method. CTC contains a phenolic hydroxyl group, an enol group and a dimethylamino group and the hydroxyl group in the OAP structure can be used as a latent coordination site so that OAP amino group, and phenolic hydroxyl group and enol group of CTC undergo intermolecular hydrogen bond interaction. OAP hydroxyl group and CTC dimethylamino group undergo a reaction so that multiple binding sites between OAP and CTC exist and the prepared molecularly imprinted polymer has very high CTC selectivity and bonding capability. The sensor can be used for on-site fast detection, does not need specimen pre-treatment, and has a low cost, simple processes and high sensitivity. The sensor has a detection linear range of 20-610nM and sensitivity of 15nM.

Description

technical field [0001] The invention belongs to the field of electrochemical analysis of antibiotic residues, and relates to molecular imprinting technology, in particular to an aureomycin molecular imprinting sensor and a preparation method thereof. Background technique [0002] Chlortetracyline (CTC) is a kind of tetracycline spectrum antibiotics, which can be extracted from Streptomyces culture fluid or produced by semi-synthetic method, and can effectively inhibit Gram-positive bacteria, negative bacteria, Rickettsia, etc. Wide range of antibacterial. It can be widely used in the prevention and treatment of fish and poultry diseases. Adding it to feed can increase the daily weight gain and feed conversion rate of poultry and livestock. However, due to drug abuse, aureomycin residue in animal food constitutes a potential hazard to human health. Most countries have made strict restrictions on the residues of aureomycin in animal foods. The No. 235 document "The Maximum R...

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Application Information

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IPC IPC(8): G01N27/30G01N27/48
Inventor 张娟琨高杨王伟刘英姿陶强
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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