This invention relates to a method for identifying and detecting urinary biomarkers of whole-grain wheat intake. These biomarkers include
glycine conjugates of 3,5-dihydroxybenzoic acid,
sulfate conjugates of o-aminophenol,
sulfate conjugates of 2-
acetaminophen,
sulfate conjugates of 3,5-dihydroxyphenylvaleric acid, glucuronic acid conjugates of o-aminophenol, or glucuronic acid conjugates of 2-
acetaminophen. The invention also relates to a high-
throughput screening method for urinary biomarkers of whole-grain wheat intake. A whole-grain wheat dietary intervention experiment was designed, and through comparative analysis of human urinary metabolic profiles, non-
targeted metabolomics technology was used to screen for urinary
metabolic biomarkers of whole-grain wheat, providing a method for accurately assessing whole-grain wheat intake.