Aluminum ion fluorescent probe and preparation method thereof

A technology of fluorescent probes and aluminum ions, applied in the field of fluorescent probes, can solve the problems of complex operation process, long detection time, high requirements for instruments, etc., and achieve the effect of good selectivity

Pending Publication Date: 2019-06-21
YULIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these testing methods have many disadvantages, such as high requirements for instruments, complicated operation process, long detection time, etc.
Compared with such traditional methods, the detection of metal ions by the fluorescent probe method has more prominent advantages, such as fast test speed, low cost, high selectivity and sensitivity, and convenient operation. There are reports, so it is necessary to develop such fluorescent probes to expand the application range of fluorescent probes and better detect aluminum ions

Method used

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  • Aluminum ion fluorescent probe and preparation method thereof
  • Aluminum ion fluorescent probe and preparation method thereof
  • Aluminum ion fluorescent probe and preparation method thereof

Examples

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Effect test

Embodiment 1

[0040] A preparation method for an aluminum ion fluorescent probe, comprising the following steps:

[0041] Step 1: Add 10g of hexamethylenetetramine and 5g of 7-hydroxycoumarin to a clean 250mL round-bottomed flask in sequence, then add 50mL of glacial acetic acid, stir the reactants well, adjust the reaction temperature to 95°C and heat Reflux for 5 hours, then transfer 75 mL of prepared hydrochloric acid with a mass concentration of 20% to the reactant, adjust the temperature to 60°C and heat for 30 minutes, then place the reactant to cool, and extract the reacted liquid with the organic solvent diethyl ether (50mL×3), combined the extracted organic matter, then added anhydrous MgSO 4 Carry out drying, the organic matter after drying is concentrated and obtain crude product, finally with dehydrated alcohol, crude product recrystallization, obtains 1.25g pale yellow strip solid, i.e. 7-hydroxycoumarin-8-aldehyde, productive rate is 21 %.

[0042] The NMR characterization d...

Embodiment 2

[0046] A preparation method for an aluminum ion fluorescent probe, comprising the following steps:

[0047] Step 1: Add 5g of hexamethylenetetramine and 5g of 7-hydroxycoumarin to a clean 250mL round-bottomed flask in turn, then add 50mL of glacial acetic acid, stir the reactants well, adjust the reaction temperature to 95°C and heat Reflux for 4 hours, then transfer 75 mL of prepared hydrochloric acid with a mass concentration of 20% to the reactant, adjust the temperature to 40°C and heat for about 50 minutes, then place the reactant to cool, and extract the reacted substance with diethyl ether, an organic solvent. liquid (50mL×3), combined the extracted organic matter, then added anhydrous MgSO 4 Carry out drying, the organic matter after drying is concentrated and obtain crude product, finally use dehydrated alcohol to crude product recrystallization, thereby obtain 0.65g pale yellow strip solid, i.e. 7-hydroxycoumarin-8-aldehyde, productive rate is 11%.

[0048] Step 2,...

Embodiment 3

[0050] A preparation method for an aluminum ion fluorescent probe, comprising the following steps:

[0051] Step 1: Add 7.5g of hexamethylenetetramine and 5g of 7-hydroxycoumarin to a clean 250mL round-bottomed flask in sequence, then add 50mL of glacial acetic acid, stir the reactants thoroughly, and adjust the reaction temperature to 95°C Heat to reflux for 6h, then transfer 75mL of prepared hydrochloric acid with a mass concentration of 20% to the reactant, adjust the temperature to 80°C and heat for about 20min, then place the reactant to cool, and extract the reactant with an organic solvent ether liquid (50mL×3), combined the extracted organic matter, then added anhydrous MgSO 4 Carry out drying, the organic matter after drying is concentrated and obtain crude product, finally use absolute ethanol to crude product recrystallization, thereby obtain 0.91g pale yellow strip solid, i.e. 7-hydroxycoumarin-8-aldehyde, productive rate is 16%.

[0052] Step 2, first add 0.38g ...

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Abstract

The invention discloses an aluminum ion fluorescent probe and a preparation method thereof. The method comprises the following steps: synthesizing 7-hydroxycoumarin-8-aldehyde by taking hexamethylenetetramine, 7-hydroxycoumarin and glacial acetic acid, and synthesizing the aluminum ion fluorescent probe by utilizing 7-hydroxycoumarin-8-aldehyde and ortho-aminophenol. The structure of the aluminumion fluorescent probe is represented by a nuclear magnetic spectrum and a mass spectrum; the spectral quality of the fluorescent probe is analyzed through an ultraviolet spectrum and a fluorescent spectrum; the result indicates that the fluorescent probe has high selectivity and is not interfered by other co-existing ions when identifying aluminum ions; and the probe generates coordination interaction with the aluminum ions according to the ratio of 1:1 and the reaction has reversibility. Therefore, the fluorescent probe can be applied to detection on the aluminum ions well.

Description

technical field [0001] The invention belongs to the technical field of fluorescent probes, and in particular relates to an aluminum ion fluorescent probe and a preparation method thereof. Background technique [0002] In nature, aluminum is the most abundant metal element in the earth's crust, accounting for about 8% of the total elements in the earth's crust. It mainly exists in aluminosilicate ores, as well as cryolite and bauxite. With the continuous development of modern industry, people are using aluminum in many fields. Although the use of aluminum has accelerated the progress of civilization, the negative effects of aluminum ions have become increasingly prominent. For example, the central nervous system and immune system will be damaged due to excessive concentration of aluminum ions, which will lead to cell death, decreased activity of biological enzymes, etc., leading to diseases such as Alzheimer's disease and Parkinson's disease. In addition, it will also inhibi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/16C09K11/06G01N21/64
Inventor 杨文生
Owner YULIN UNIV
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