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Fermentation technology for increasing the aureomycin yield by adding metal ions

A technology of adding metal and fermentation process is applied in the field of fermentation process optimization of chlortetracycline, and achieves the effects of low production cost, simple operation, and improvement of fermentation level and yield.

Inactive Publication Date: 2014-03-05
ZHUMADIAN HUAZHONG CHIA TAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing fermentation production of aureomycin is only a relatively conventional production method, and there is still room for improvement in the fermentation unit and output

Method used

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  • Fermentation technology for increasing the aureomycin yield by adding metal ions
  • Fermentation technology for increasing the aureomycin yield by adding metal ions
  • Fermentation technology for increasing the aureomycin yield by adding metal ions

Examples

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Comparison scheme
Effect test

Embodiment 1

[0009] Example 1: Inoculate the slant spores of the mother bottle to the slant of the child bottle, and cultivate them for 3-5 days at a temperature of 32±1°C and a humidity of 40-65%; take the spores of the child bottle and inoculate them into the seed medium, cultivate at 28°C 20-26h; Inoculate in a fermentation shaker flask with 12% inoculum amount, culture at 28°C, 200r / min, for 166h. When preparing the fermentation medium, zinc sulfate and ferric chloride were added thereto, so that the concentrations of zinc ions and iron ions in the fermentation shake flask were 500ppm and 1ppm respectively.

Embodiment 2

[0010] Example 2: inoculate the slant spores of the mother bottle to the slant of the child bottle, and cultivate for 3-5 days under the conditions of temperature 32±1°C and humidity 40-65%; take the spores of the child bottle and inoculate them into the seed medium, cultivate at 28°C 20-26h; Inoculate in a fermentation shaker flask with 12% inoculum amount, culture at 28°C, 200r / min, for 166h. When preparing the fermentation medium, zinc sulfate and ferric chloride were added thereto, so that the concentrations of zinc ions and iron ions in the fermentation shake flask were 1000ppm and 0.5ppm respectively.

Embodiment 3

[0011] Example 3: Inoculate the slant spores of the mother bottle to the slant of the child bottle, and cultivate them for 3-5 days at a temperature of 32±1°C and a humidity of 40-65%; take the spores of the child bottle and inoculate them into the seed medium, cultivate at 28°C 20-26h; Inoculate in a fermentation shaker flask with 12% inoculum amount, culture at 28°C, 200r / min, for 166h. When preparing the fermentation medium, zinc sulfate and ferric chloride were added thereto so that the concentrations of zinc ions and iron ions in the fermentation shake flask were 1000ppm and 1ppm respectively.

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Abstract

The invention provides a fermentation technology for increasing the aureomycin yield by adding metal ions. Certain amounts of zinc sulfate and ferric trichloride are added into a fermentation culture medium of aureomycin, thus achieving an objective of increasing the aureomycin yield. According to the technology, 500 ppm of the zinc sulfate and 1 ppm of the ferric trichloride are added into the conventional fermentation culture medium. The fermentation level by the fermentation technology provided by the invention is higher than the original fermentation level. The pilot potency and the yield are both increased, thus laying good foundations for industrial scale-up and application in the next stage.

Description

technical field [0001] The invention relates to a method for optimizing the fermentation process of aureomycin, in particular to a fermentation process for increasing the output of aureomycin by adding metal ions. Background technique [0002] The fermentative production method of aureomycin is to use Streptomyces aureus as the starting strain, through pure culture and three-stage fermentation to produce aureomycin. The specific process steps include the preparation of sandy soil spores, the preparation of slant spores in the mother bottle, and the preparation of slant spores in the child bottle. , Primary seed preparation, secondary seed preparation, fermentation preparation. The existing fermentation production of aureomycin is just some conventional fermentation production methods, and there is still room for improvement in the fermentation unit and output. [0003] Metal ions are trace elements present in the aureomycin fermentation medium, which have a huge impact ...

Claims

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Application Information

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IPC IPC(8): C12P29/00C12R1/49
Inventor 张雪峥刘世民孙庆峰
Owner ZHUMADIAN HUAZHONG CHIA TAI
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