Aspergillus oryzae LJ366 strain used for degrading aureomycin

A technology of LJ366 and chlortetracycline, applied in the field of microorganisms

Inactive Publication Date: 2013-12-04
BEIJING INSTITUTE OF TECHNOLOGYGY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And the research about aspergillus oryzae degradation ...

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  • Aspergillus oryzae LJ366 strain used for degrading aureomycin
  • Aspergillus oryzae LJ366 strain used for degrading aureomycin
  • Aspergillus oryzae LJ366 strain used for degrading aureomycin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Screening and identification of aureomycin-degrading strain LJ366

[0025] 1. Material preparation

[0026] Bacteria sample source: aureomycin residue from the aureomycin manufacturer.

[0027] Martin's Broth: KH 2 PO 4 1.0g, MgSO 4 ·7H 2 O0.5g, peptone 5g, glucose 10g, dissolved in 1000mL tap water, sterilized by damp heat at 121°C for 15min. Add agar at a ratio of 13%-18% to obtain Martin's solid medium.

[0028] Inorganic salt liquid medium: (NH 4 ) 2 SO 4 2.0g, K 2 HPO 4 0.5g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O0.5g, NaCl0.2g, CaCl 2 0.1g, FeSO 4 ·7H 2 O0.01g, EDTA0.015g, dissolved in 1000mL tap water, sterilized by moist heat at 121℃ for 20min.

[0029] In the inorganic salt liquid medium, add agar at a ratio of 13%-18% to obtain an inorganic salt solid medium.

[0030] 2. Experimental instruments and equipment

[0031] Shimadzu LC-20A high-performance liquid chromatography, UNICO UV-2600A ultraviolet-visible spectrophotometer, Mettler Toledo ML20...

Embodiment 2

[0064] Growth and degradation experiments of strain LJ366 in medium with aureomycin as sole carbon source

[0065] 1. Growth experiments on medium with different concentrations of mycin

[0066] The preparation uses chlortetracycline as the sole carbon source and the concentrations of chlortetracycline are 0.01g / L, 0.05g / L, 0.10g / L, 0.50g / L, 5.00g / L, 10.00g / L, 15.00g / L inorganic salt solid medium. Pick a few LJ366 colonies, streak on the inorganic salt solid medium plate, and culture at room temperature in the dark. It was observed that on the 3rd day of cultivation, mycelium grew on plates with aureomycin concentrations of 0.01g / L, 0.05g / L, 0.1g / L, 0.5g / L and 5.0g / L, and the growth good. On the 15th day of culture, mycelium grew on the plate with the concentration of aureomycin at 10.00g / L, which indicated that the strain LJ366 could tolerate the concentration of aureomycin at 10.00g / L.

[0067] 2. Degradation experiment of strain LJ366 on aureomycin in shake flask

[00...

Embodiment 3

[0071] Degradation of aureomycin in bacterial residue by strain LJ366

[0072] 1. Strain activation and bacteria residue pretreatment

[0073] Strain LJ366 was activated with Martin's medium. After the fungus residue is crushed by a crusher, it is divided into two parts, one part is sterilized with moist heat at 121°C for 20 minutes to 30 minutes, and the other part is not subjected to heat treatment.

[0074] 2. Inoculation and fermentation culture

[0075] Inoculate the activated strain LJ366 into the above-mentioned pretreated fungus residue according to the inoculum amount of 5%-7%, respectively, and add a certain amount of carbon source such as bran, sawdust or straw, etc., stir evenly, and put it into the fermentation In the container, solid fermentation is carried out at a temperature of 30°C-35°C, with regular stirring and sampling.

[0076] 3. Determination of pH value of bacteria residue during fermentation

[0077] On the 0d, 3d, 6d, 9d, and 15d of fermentation,...

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Abstract

The invention relates to an Aspergillus oryzae LJ366 strain used for degrading aureomycin and belongs to the microbial technical field. The bacterial strain has already been preserved in the China General Microbiological Culture Collection Center on Aug 27, 2013 and the preservation number is CGMCC No.8078. The ITS gene sequence of the Aspergillus oryzae LJ366 strain used for degrading the aureomycin is composed of 593 bases. The bacterial strain can degrade the aureomycin and can be used for processing residual aureomycin in solid waste and liquid.

Description

technical field [0001] The invention relates to a strain of Aspergillus oryzae LJ366 capable of degrading aureomycin, belonging to the technical field of microorganisms. Background technique [0002] Chlortetracycline (CTC) belongs to tetracycline antibiotics. It has the advantages of broad antibacterial spectrum, growth promotion at low concentration and low cost. It is widely used in livestock, poultry and aquaculture and other industries. After aureomycin enters the animal body, most of it cannot be completely absorbed by the body, but is excreted in the form of the original form or metabolites through the feces, posing a threat to the safety of the ecological environment. At the same time, in the process of chlortetracycline fermentation and production, a large amount of waste residue and wastewater containing chlortetracycline residues will be produced. If the residual chlortetracycline is not properly treated and discharged into the environment, it will have a negative...

Claims

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Application Information

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IPC IPC(8): C12N1/14A62D3/02C12R1/69A62D101/28
Inventor 李艳菊顾金刚梁山琴顾晓丽龚骞李世贵龚明波刘文丽
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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