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Penicillium spinulosum LJ220 strain for degrading aureomycin

A technology of LJ220 and Penicillium minor, applied in the field of microorganisms to achieve good adaptability

Inactive Publication Date: 2012-10-17
BEIJING INSTITUTE OF TECHNOLOGYGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And relevant penicillium punctatus is to the research of degrading aureomycin aspect, there is no report both at home and abroad

Method used

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  • Penicillium spinulosum LJ220 strain for degrading aureomycin
  • Penicillium spinulosum LJ220 strain for degrading aureomycin
  • Penicillium spinulosum LJ220 strain for degrading aureomycin

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Experimental program
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Effect test

Embodiment 1

[0027] 1. A screening method for Penicillium punctatus LJ220 that degrades aureomycin

[0028] 1.1 Material preparation

[0029] Source of bacteria samples: collected from the waste of aureomycin production enterprises.

[0030] Medium:

[0031] Inorganic salt solid medium: (NH4) 2 SO 4 2.00g, K 2 HPO 4 0.50g, KH 2 PO 4 0.50g, MgSO 4 7H2O 0.50g, NaCl 0.20g, CaCl 2 0.1g, FeSO 4 0.01g, 0.015g EDTA, 13.00g agar, 2.00g / L glucose, dissolved in 1000ml distilled water. After sterilizing at 115°C for 20 minutes, add 2.00 g / L aureomycin to the ultra-clean workbench.

[0032] Martin's medium: peptone 6.0g, glucose 10.0g, KH 2 PO 4 1.0g, MgSO 4 ·7H2O 0.5g, dissolved in 1000ml of distilled water. Sterilize at 115°C for 20 minutes.

[0033] Select medium: (NH4) 2 SO 4 2.00,K 2 HPO 4 0.50, KH 2 PO 4 0.50, MgSO 4 7H2O 0.50, NaCl 0.20, CaCl 2 0.10, FeSO 4 0.01g, 0.015g of EDTA, dissolved in 1000ml of distilled water. Sterilize at 115°C for 20 minutes. After cooling, ...

Embodiment 2

[0073] Degradation of Chlortetracycline as the Sole Carbon Source by Strain LJ220

[0074] The strain LJ220 was inserted into the inorganic salt liquid medium with chlortetracycline as the only carbon source and the content of chlortetracycline was 4.00g / L, and cultured in shake flasks, and the content of chlortetracycline in the culture medium was determined. The degradation rate of LJ220 to the sole carbon source aureomycin can reach 31.60% through measurement. The determination method is: take 1mL of culture fluid, add 5mL of methanol, centrifuge at 8000r / min for 5min, take the supernatant and filter it with a 22μm filter membrane, use high performance liquid chromatography to measure the content of aureomycin in the filtrate and calculate the degradation rate .

Embodiment 3

[0076] Experiment on Treatment of Chlortetracycline Residue by Strain LJ220

[0077] Activate the bacterial strain LJ220 with Martin's medium, then inoculate it into fresh chlortetracycline slag, carry out dark culture under room temperature, then take samples regularly to measure the residual amount of chlortetracycline in the bacterium slag. The degradation rate of aureomycin was 80.02%. The determination method is: accurately weigh 5.00 g of the bacteria residue, extract with 50 mL of acetone: hydrochloric acid solution (4mol / L): water = 13: 1: 6 extract, centrifuge the extract at 8000 r / min for 5 min, and take the The clear liquid was filtered with a filter membrane of 22 μm, and the content of aureomycin in the filtrate was measured by high performance liquid chromatography, and the degradation rate was calculated.

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Abstract

The invention relates to a Penicillium spinulosum LJ220 strain for degrading aureomycin, belonging to the technical field of microorganism. The Penicillium spinulosum LJ220 strain disclosed herein is preserved in China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.5754 on 14 Feb, 2012. The gene sequence of the strain is obtained by genome DNA extraction, rDNA-ITS sequence-based amplification and complete sequence determination and analysis. The LJ220 strain can grow in a medium with aureomycin as the sole carbon source. The strain is convenient for usage, and has efficient degradation on residual aureomycin in bacteria residues.

Description

technical field [0001] The invention relates to a strain of Penicillium spinulosum LJ220 capable of degrading aureomycin, belonging to the technical field of microorganisms. Background technique [0002] Chlortetracycline can prevent various animal diseases and is widely used in veterinary clinics and animal husbandry. Chlortetracycline will produce a large amount of bacteria residue and waste water during the fermentation production process. If the treatment is not perfect, a large amount of Chlortetracycline will enter the environment, which will have toxic and inhibitory effects on the ecological environment and many organisms, and may also induce drug-resistant bacteria. and drug resistance genes, causing potential harm to human health. Therefore, the detoxification treatment of chlortetracycline waste has become a major problem to be solved urgently in the residual pollution of chlortetracycline waste and the sustainable development of production enterprises. At the s...

Claims

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Application Information

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IPC IPC(8): C12N1/14A62D3/02C12R1/80A62D101/28
Inventor 李艳菊肖思颖董晓霞李秀珍杨正礼
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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