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Aureomycin strain breeding culture medium and strain breeding method

A strain breeding and culture medium technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve uneconomical problems, reduce the probability of pollution, improve the efficiency of strain breeding, shorten the The effect of strain selection time

Inactive Publication Date: 2015-10-21
金河生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, above-mentioned method is more and more uneconomical, even becomes the bottleneck of aureomycin production development, therefore, it is necessary to improve existing technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The improved aureomycin strain breeding medium is composed of the following components in terms of mass percentage:

[0014] Corn starch 6.0%, soybean cake powder 4.0%, yeast powder 0.2%, calcium carbonate 0.6%, ammonium sulfate 0.35%, sodium chloride 0.25%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.03%, soybean oil 4.28%, the balance for water.

[0015] Fill the culture medium in a 35mL / 250mL Erlenmeyer flask, seal the bottle mouth with 4g cotton plug, and two layers of gauze, and sterilize at 0.090-0.105Mpa, 120±1°C for 30 minutes.

[0016] After the sterilization is completed, the inoculation method: Digging method about 1cm 2 (1 / 20 of the slant of the test tube), cultured at a constant temperature of 30±1.5° C. for 6 days in a rotary shaker at 220 to 260 rpm. The cultured fermentation liquid is dark brown, thick, pH 5.6-7.6, titer not less than 16000U / ml, and can directly enter the next breeding procedure.

Embodiment 2

[0018] The improved aureomycin strain breeding medium is composed of the following components in terms of mass percentage:

[0019] Corn starch 7.0%, soybean cake powder 3.5%, yeast powder 0.25%, calcium carbonate 0.65%, ammonium sulfate 0.3%, sodium chloride 0.2%, magnesium sulfate 0.025%, potassium dihydrogen phosphate 0.025%, soybean oil 2.86%, balance for water.

[0020] Fill the culture medium in a 35mL / 250mL Erlenmeyer flask, seal the bottle mouth with 4g cotton plug, and two layers of gauze, and sterilize at 0.090-0.105Mpa, 120±1°C for 30 minutes.

[0021] After the sterilization is completed, the inoculation method: Digging method about 1cm 2 (1 / 20 of the slant of the test tube), cultured at a constant temperature of 30±1.5° C. for 6 days in a rotary shaker at 220 to 260 rpm. The cultured fermentation liquid is dark brown, thick, pH 5.6-7.6, titer not less than 16000U / ml, and can directly enter the next breeding procedure.

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Abstract

The invention discloses an aureomycin strain breeding culture medium and a strain breeding method. The culture medium comprises, by weight, 6.0%-7.5% of corn starch, 3.5%-4.5% of soybean cake powder, 0.15%-0.25% of yeast powder, 0.55%-0.65% of calcium carbonate, 0.3%-0.4% of ammonium sulfate, 0.2%-0.3% of sodium chloride, 0.02%-0.03% of magnesium sulfate, 0.02%-0.03% of monopotassium phosphate, 2.86%-4.28% of soya-bean oil and the balance water. An aureomycin strain breeding culture medium formula is improved, a strain culturing and strain fermentation cultivation second-level fermentation mode is changed into a primary fermentation mode, strain breeding time is greatly shortened, strain breeding efficiency is improved, the using quantity of shaking flasks and shaking tables is reduced, cost is lowered, the probability of strain pollution is lowered, and accordingly the culture medium and the method are suitable for large-scale strain screening.

Description

technical field [0001] The invention belongs to the field of biological fermentation, and in particular relates to a chlortetracycline strain breeding medium and a strain selection method. Background technique [0002] Aureomycin is a secondary metabolite of Streptomyces aureofaciens, a tetracyclic antibiotic, often used as a feed additive, against Gram-negative bacteria, Gram-positive bacteria, spirochetes, chlamydia, mycoplasma, Creutzia, etc. all have antibacterial effects, and are a broad-spectrum antibiotic, which can not only resist disease, but also increase weight, with less adverse reactions. With the in-depth research on tetracyclic antibiotics in the field of biopharmaceuticals, the current aureomycin products centered on chlortetracycline hydrochloride and feed-grade chlortetracycline are very suitable for domestic and foreign breeding environments. More and more feed factories use chlortetracycline As the drug of choice for antibiotics, mycomycin makes its mark...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/485
CPCC12N1/20
Inventor 谢昌贤李兰枝刘运添王鹏飞
Owner 金河生物科技股份有限公司
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