Detection method for antibiotic residual in livestock meat

A detection method and antibiotic technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of affecting the detection results, low, less than 70%, and achieve high detection accuracy, high recovery rate, and simple processing steps Effect

Inactive Publication Date: 2017-09-19
SUZHOU POLYTECHNIC INST OF AGRI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the existing detection of antibiotic residues in animals, it is found that when the detection sample contains high protein, it will seriously affect the detection results, such as the Chinese patent No. 201310334436.X "A method for the determination of tetracycline antibiotic residues in protein-rich samples Mentioned in the method ", when adopting high performance liquid chromatography to measure the content of oxytetracycline in different sample matrices, it is found that the recovery rate of oxytetracycline in water samples can be as high as 90%; and when oxytetracycline in pork tissue, The recovery rate is very low, less than 70%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Take 5g of pork and wash it, dry the surface moisture, then cut into thin slices with a thickness of about 0.1cm, and set aside;

[0023] (2) Sprinkle 7g of sodium carbonate powder on the slice, stir well,

[0024] (3) Dry the slices in an oven at 45°C until the moisture content is 30%, take out the slices, wash with distilled water for 3 times, and then continue drying until the moisture content of the slices is not higher than 10%;

[0025] (4) Crush the flakes, extract with extractant, and take the supernatant after centrifugation. The extractant is 24 mL of a mixed solution of tert-butyl methyl ether and acetonitrile. Specifically, the volume ratio of tert-butyl methyl ether to acetonitrile is 1:2 .

[0026] (5) After the supernatant passes through the microporous membrane, the antibiotic residues are determined by ultra-high performance liquid chromatography coupled with mass spectrometry; the pore size of the microporous membrane is 0.3 µm

[0027] Use an ul...

Embodiment 2

[0032] (1) Take 5.0g of beef and wash it, dry the surface moisture, then cut into thin slices with a thickness of about 0.5cm, and set aside;

[0033] (2) Sprinkle 3mL of sodium bicarbonate aqueous solution evenly on the slice, of which the mass of sodium bicarbonate is 10g;

[0034] (3) Dry the slices in an oven at 60°C until the moisture content is 50%, take out the slices, wash them twice with distilled water, and then continue drying until the moisture content of the slices is not higher than 10%;

[0035] (4) Crush the flakes, extract with extractant, and take the supernatant after centrifugation. The extractant is a mixed solution of tert-butyl methyl ether and acetonitrile, and the volume ratio of tert-butyl methyl ether and acetonitrile is 1:5.

[0036] (5) After passing the supernatant through a microporous membrane with a pore size of 0.45 μm, use ultra-high performance liquid chromatography coupled with mass spectrometry to determine antibiotic residues;

[0037] R...

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Abstract

The invention discloses a detection method for antibiotic residual in livestock meat. The detection method comprises the following steps: cleaning the livestock meat, blowing moisture on a surface to be dry, and then, cutting the livestock meat into slices of which the thickness is about 0.1-1.0cm for standby; spraying solid weak base on the slices or spraying weak base aqueous solution on the slices; putting the slices in a drying oven to be dried at the temperature of 30-60 DEG C, taking out the slices when the moisture content is 30-50%, washing in distilled water for 1-3 times, and then, continuously drying until the moisture content of the slices is not higher than 10%; grinding the slices, adding extractant for extraction, carrying out centrifugation, and taking supernate; after the supernate passes through a microfiltration membrane, adopting a ultra-high performance liquid chromatography and mass spectrum combined method to measure the antibiotic residual, wherein the antibiotics can be oxytetracycline, tetracycline, doxycycline and aureomycin. The detection method is characterized in that the influence of high protein muscle tissues on an antibiotic recovery rate can be avoided especially in the early-stage treatment of a sample, and detection accuracy is high.

Description

technical field [0001] The invention relates to a method for detecting veterinary drug residues, in particular to a method for detecting antibiotic residues in poultry meat. Background technique [0002] The abuse of antibiotics in animal feeding is serious. Long-term use of animal foods with excessive antibiotic content will cause acute or chronic poisoning and directly endanger human health. Existing antibiotics, especially tetracycline antibiotics are cheap and widely used. [0003] In the existing detection of antibiotic residues in animals, it is found that when the detection sample contains high protein, it will seriously affect the detection results, such as the Chinese patent No. 201310334436.X "A method for the determination of tetracycline antibiotic residues in protein-rich samples Mentioned in the method ", when adopting high performance liquid chromatography to measure the content of oxytetracycline in different sample matrices, it is found that the recovery ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 司文会方志成高岳阙小峰吴晨奇余雁
Owner SUZHOU POLYTECHNIC INST OF AGRI
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