Method for detecting fungaltoxin by using photonic crystal microsphere flow type chip

A technology of photonic crystal microspheres and mycotoxins, which is applied in low-cost detection, fast mycotoxins, and high-throughput fields. It can solve the problems of not being able to meet the requirements of on-site, fast, sensitive, stable and low-cost, etc., to expand the linear range of detection, Good detection specificity, saving reagents and samples

Inactive Publication Date: 2013-01-16
NANJING NORMAL UNIVERSITY
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Problems solved by technology

Due to the diversity of agricultural product samples and their pollution sources, the detection of mycotoxins in agricultural products and t

Method used

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  • Method for detecting fungaltoxin by using photonic crystal microsphere flow type chip
  • Method for detecting fungaltoxin by using photonic crystal microsphere flow type chip
  • Method for detecting fungaltoxin by using photonic crystal microsphere flow type chip

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Embodiment 1

[0047] Corn, wheat and peanuts were pulverized by a high-speed pulverizer to a particle size of less than 1mm, and 5 grams of each sample was weighed into a 100ml volumetric flask. Prepare different concentration gradient mycotoxin standard substances with methanol as solvent, and mix the samples thoroughly. These samples were placed in a fume hood overnight to allow the solvent to evaporate. The added standard was extracted by 6ml of chloroform. After shaking for 1 h, filter with whatman filter paper. The filtrate was collected and placed in a dry box at 65 °C to evaporate the solvent. The extracted toxin sample was dissolved in 2 mL, 0.01 M, pH 7.4 PBS buffer. At the same time, extract the control sample without adding standard substance according to the above method.

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Abstract

The invention relates to a method for detecting fungaltoxin by using a photonic crystal microsphere flow type chip. According to the method, a photonic crystal microsphere is used as a fungaltoxin probe carrier; the surface of the microsphere is directly subjected to competitive immune, so that a plurality of fungaltoxins are detected; aflatoxin, Fumonisins and penicillium citrinum toxin are used as detection objects; a fluorescein isothiocyanate marked antibody competes with an antigen fixed on the surface of the microsphere to obtain own antigens; decoding is performed by a confocal fluorescence scanner and a reflection spectrum of a photonic crystal; and the three fungaltoxins can be detected at the same time. According to the method, the detection limit to the aflatoxin, the Fumonisins and the penicillium citrinum toxin is 0.001 ng/mL; the linear detection range is 0.001 to 10 ng/mL; only 10 mu L test sample is needed; and the whole analysis process is shorter than 3 hours.

Description

technical field [0001] The invention relates to a rapid, high-throughput and low-cost detection method for mycotoxins in agricultural products, feed and food. Specifically by typical aflatoxins in cereals (AFB 1 ), fumonisins (FB 1 ) and citrinin (CIT) mycotoxins as detection objects, using the differences in the reflection peak positions of the three photonic crystal microspheres to physically encode the three photonic crystal microspheres, and constructing liquid on the surface of the photonic crystal microspheres by competitive immunity Phase chip detection technology. Background technique [0002] Mycotoxins are secondary metabolites secreted by toxin-producing fungi that can cause various damages to humans and animals. So far, more than 500 kinds of mycotoxins have been discovered, and new mycotoxins are constantly being discovered. Among them, the mycotoxins that are harmful to humans and animals, have strong toxicity and high pollution frequency mainly include afl...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N21/64
Inventor 李建林邓国哲徐坤孙越陈宇
Owner NANJING NORMAL UNIVERSITY
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