Immobilization method of penicillium citrinum

Abstract

The invention discloses an immobilization method of penicillium citrinum, which comprises the steps that a pretreated fiber material serves as an immobilization medium; a production strain, namely the penicillium citrinum of nuclease P1 is activated, inoculated into a fermentation container, and cultured for 24-60h, so that the strain is adsorbed on the fiber material in a culturing process. In the method, the fiber material used by an immobilization cell is pretreated correspondingly, and is low in cost, nontoxic, easy to obtain and simple in preparation process when serving as an immobilization material; the method is suitable for large-scale production. The pretreated fiber material has larger porosity and specific surface area, so that cells immobilized in unit volume are more, the advantage of high density fermentation is ensured, the fiber material has good mass transfer property, and a fermentation period is shortened.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to view more

AI Technical Summary

Benefits of technology

This new type of fibers called benfibrillar) have several benefits compared with previous types like cellulose or synthetic polymer materials that were previously commonly found in biological systems such as bacteria. These benefiting properties include being nontoxic for cells without harmful side effects, having strong physical structures, resistant against chemicals, easy handling during manufacturing processes, allowing it to support itself over multiple cycles of use (a long lifespan), providing excellent mechanic stability when exposed to harsh environments, enabling them to grow well even at extreme temperatures, reducing their weight and size making them easier to transport than other alternatives.

Problems solved by technology

This patented technical problem addressed by the patented describes how to efficiently fix certain substances like penicilin (penicillopeptide) from various sources while maintain their original structure without losing them during manufacture processes. Current methods involve expensive chemical synthesis techniques involving multiple steps, resulting in increased environmental impact. Therefore, there needs effective alternative ways to effectively fix these compounds onto fibers instead of traditional methods requiring complicated procedures.

Images